Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.

Mark Bates, Jan Keller-Findeisen, Adrian Przybylski, Andreas Hüper, Till Stephan, Peter Ilgen, Angel R Cereceda Delgado, Elisa D'Este, Alexander Egner, Stefan Jakobs, Steffen J Sahl, Stefan W Hell

May 2022 Nat Methods

Synopsis of Social media discussions

The discussions express strong enthusiasm for the article's technical advancements, with posts describing the impressive imaging of mitochondria and modeling techniques, such as 'incredible complexity embedded inside elegance and simplicity.' The tone underscores admiration for the work's scientific beauty and potential, using words like 'beautiful,' 'amazing,' and 'incredible,' which convey both appreciation and recognition of its significance in expanding microscopy capabilities.

A
Agreement
 Strong agreement

Many discussions praise the publication as innovative and impactful, with phrases like 'incredible complexity inside elegance and simplicity' and 'latest work in 3D super-resolution microscopy,' indicating strong alignment with the research's significance

I
Interest
 High level of interest

Posts demonstrate high curiosity, sharing links, videos, and emphasizing technological advances, reflected in expressions like 'such a beautiful work' and referencing the detailed imaging techniques used.

E
Engagement
 High engagement

Participants actively engage with the methodology and implications, mentioning specific imaging results, techniques like spline PSF models, and applications in cellular structures, showing deep interest and understanding.

I
Impact
 Moderate level of impact

While some posts highlight the potential for advancing biological imaging, the overall perception leans toward a recognition of its importance, though not explicitly claiming it will revolutionize the field.

Social Mentions

YouTube

3 Videos

Facebook

2 Posts

Twitter

32 Posts

Metrics

Video Views

563

Total Likes

144

Extended Reach

186,323

Social Features

37

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

High-Precision 3D Imaging of Mitochondrial Protein Using Advanced Super-Resolution Microscopy

High-Precision 3D Imaging of Mitochondrial Protein Using Advanced Super-Resolution Microscopy

3D animation of the distribution of the mitochondrial protein Mic60 in a COS7 cell. Mic60 is part of the MICOS complex responsible for inner mitochondrial structure. This technique achieves high accuracy in 3D imaging by dynamically modeling the point spread function, enabling detailed cellular structural studies.

JakobsLab

June 3, 2022

282 views

High-Precision 3D Microscopy of Mitochondrial Protein Distribution in Cells

High-Precision 3D Microscopy of Mitochondrial Protein Distribution in Cells

3D animation of the distribution of the mitochondrial protein Mic60 in a U2 OS cell. Mic60 is a component of the MICOS complex responsible for inner mitochondrial architecture. The data is from our publication on 4PiSTORM imaging accuracy and precision.

JakobsLab

June 3, 2022

149 views

Enhanced 3D Neuronal Imaging with Dynamic 4PiSTORM Technology

Enhanced 3D Neuronal Imaging with Dynamic 4PiSTORM Technology

3D animation of betaII-spectrin in a primary neuron imaged with advanced 4PiSTORM microscopy, demonstrating high precision and accuracy in biological imaging. This technique captures detailed structures in neurons and mitochondria, enabling improved biological investigations.

JakobsLab

May 23, 2022

132 views

  • Till Stephan
    @stephan_till (Twitter)

    The 4Pi-STORM by @wmarkbates flies
    view full post

    December 14, 2023

    7

  • Till Stephan
    @stephan_till (Twitter)

    One of my favorite 3D SMLM recordings of mitochondria is now on Youtube. https://t.co/qZyLdddqyi https://t.co/WZ0PFeLuMO https://t.co/JiudEB3Yr5
    view full post

    June 5, 2022

    12

  • JakobsLab
    @JakobsLab (Twitter)

    ... and in a U-2 OS cell. It is always amazing to see how much the distribution of the same protein can differ between different cell lines. Another example of how versatile mitochondria really are!
    view full post

    June 3, 2022

    5

  • JakobsLab
    @JakobsLab (Twitter)

    We just uploaded new videos to our YouTube Channel: Data from 4Pi STORM imaging of the MICOS subunit Mic60 in a COS-7 cell ... (1/3) https://t.co/Fzs0absE6Z
    view full post

    June 3, 2022

    13

  • Peter I.
    @pitvandyck (Twitter)

    RT @JakobsLab: We have shown you our #SpectrinWaltz with the data from our latest publication (Bates et al., 2022) already here on Twitter.…
    view full post

    May 23, 2022

    1

  • JakobsLab
    @JakobsLab (Twitter)

    We have shown you our #SpectrinWaltz with the data from our latest publication (Bates et al., 2022) already here on Twitter...but NOW you can watch it in all its glory (up to 4k) on our YouTube channel
    view full post

    May 23, 2022

    7

    1

  • DZ Gong | 龚 | ゴン | 공
    @dz_gong (Twitter)

    Paper read today #83 from @StefanHellLabs: Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models https://t.co/ELV3Ex4acZ Such an beautiful work. Incredible complexity embedded inside elegance and simplicity. https://t.co/8dqOpx3Yw3
    view full post

    May 18, 2022

  • Nano IJ
    @nanomicroscopy (Twitter)

    Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models https://t.co/S5gFdq5kpL https://t.co/ErDbIrERDv
    view full post

    May 17, 2022

    2

  • Super Res Microscopy
    @SRMicro_papers (Twitter)

    Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models by Mark Bates https://t.co/s0lThcpNQX
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    May 17, 2022

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    @Educators_Link (Twitter)

    Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models https://t.co/avrs9Hh0F0
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    May 16, 2022

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    @katonalab (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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    @realAbbasRizvi (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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    October 25, 2021

    19

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    @sciencethecat (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • PromisingPreprints
    @PromPreprint (Twitter)

    Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models https://t.co/iCe1htlCq1
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    @Honigmann_Lab (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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    October 24, 2021

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    @ChienHuaTu1 (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • Vicidomini Lab
    @VicidominiLab (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • Roman Tsukanov
    @RomanTsukanov (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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    @IlariaTesta4 (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • JakobsLab
    @JakobsLab (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • Peter I.
    @pitvandyck (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • biophotonicat
    @biophotonicat (Twitter)

    Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models (relevance: 75%) https://t.co/5mpXSWWEOc #biophotonics #biomedicaloptics https://t.co/VotsvVXpsS
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  • Sam Peng
    @CTSPeng (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • Tino Pleiner
    @pleinerlab (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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  • Amit Koikkarah Aji
    @MalluKidAtYale (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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    October 19, 2021

    19

  • Francisco Balzarotti
    @BalzarottiFran (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
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    October 19, 2021

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  • Benjamin Compans
    @bcompans (Twitter)

    RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection
    view full post

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  • Nano IJ
    @nanomicroscopy (Twitter)

    Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models https://t.co/b7jWQzC1A2
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  • Mark Bates
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  • bioRxiv Biophysics
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Abstract Synopsis

  • The study introduces a new 4PiSTORM microscopy technique that achieves high precision 3D imaging by dynamically modeling the point spread function (PSF) with spline-based methods, simplifying the optical setup.
  • This approach accurately captures the temporal changes in the PSF, allowing the system to reach theoretical limits of localization precision (~2.3nm) and reduce artifacts like phase wrapping, thereby enhancing image quality.
  • The improved 4PiSTORM technique was demonstrated by successfully imaging protein and nucleic acid structures in primary neurons and mitochondria, showcasing its potential for detailed biological investigations.]