Synopsis of Social media discussions
The discussions reflect enthusiasm and recognition of the method’s potential, referencing specific achievements like assembling a 40 kb genome from 52 parts and noting protocols for large DNA construct assembly. Language such as 'optimized,' 'rapid,' and 'viable phage' convey excitement about the advancement's significance and practical applications.
Agreement
Moderate agreementMost discussions support the findings, highlighting successful assembly of the large genome and emphasizing its significance.
Interest
High level of interestDiscussions show high interest, with numerous posts referencing protocols, optimization, and broad applications of the method.
Engagement
Moderate level of engagementPosts often delve into technical details and implications, indicating moderate engagement and curiosity about the techniques.
Impact
High level of impactThe consensus underscores the high impact of this work on synthetic biology and genetic engineering, positioning it as a breakthrough.
Social Mentions
YouTube
2 Videos
10 Posts
74 Posts
Blogs
1 Articles
News
2 Articles
Metrics
Video Views
129
Total Likes
225
Extended Reach
171,971
Social Features
89
Timeline: Posts about article
Top Social Media Posts
Olivier Borkowski
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full post
ACS Synthetic Bio
Testing the boundaries of golden gate assembly:
Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragments in a single round, as shown with the 40 kb T7 bacteriophage genome.
The article shares protocols and design principles:
https://t.co/iyrTqjwphd https://t.co/Lr12ej7nlQ
view full post
Twist Bioscience
New work from @NEBiolabs researchers- Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design. #synbio @ACSPublications
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design | Synthetic Biology https://t.co/ODBWKskF5L
view full post
New England Biolabs
@NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble and transform the 40kb T7 phage genome, producing viable phage, and can be applied to rapidly engineer large and complex targets.
https://t.co/be3tpRE4JV
view full post
New England Biolabs
@NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recovering infectious phage particles after transformation! These protocols can be applied to rapidly engineer a wide variety of targets.
https://t.co/flRaLsYEby
view full post
Posts referencing the article
Efficient Assembly of Large DNA Constructs Using Golden Gate Methodology
This video discusses using Golden Gate assembly to create large DNA constructs, demonstrating the assembly of a 40 kb bacteriophage genome from 52 DNA parts in a single reaction, highlighting the method's efficiency and potential applications in synthetic biology.
June 29, 2025
44 views
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What is the consensus on Golden Gate Assembly: cycling between 16C and 37C, or only 37C? @NEBiolabs's pub: https://t.co/MCJr1ix0Kh suggests 37C only is superior but in our hands this doesn't seem to be the case. https://t.co/r2PJ6nlz5b
view full postOctober 28, 2022
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sangyoon Lee
@sylee2k (Twitter)RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
view full postAugust 2, 2022
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Biomemory
@BiomemoryLabs (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJuly 25, 2022
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Sumit Das
@iamsumitdas13 (Twitter)RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
view full postJuly 7, 2022
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1st Philekorea Technology
@1stPhilekorea (Twitter)RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
view full postJuly 7, 2022
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Jenny Brown
@JennyBrownQLD (Twitter)RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
view full postJuly 6, 2022
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T. K. Märtìn Tsuï
@XyzphineR (Twitter)I guess we can finally acknowledge the feasibility to assemble large fragments in vitro, even if one chose not to GGA it with 52 fragments all at once. https://t.co/eXt4DSJHnS
view full postJuly 6, 2022
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Wataru Fujii
@ReprodBiotech (Twitter)RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
view full postJuly 6, 2022
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New England Biolabs
@NEBiolabs (Twitter)@NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble and transform the 40kb T7 phage genome, producing viable phage, and can be applied to rapidly engineer large and complex targets. https://t.co/be3tpRE4JV
view full postJuly 6, 2022
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Davin Miller
@hitcintang (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 12, 2022
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Adam Peltan
@adampeltan (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 12, 2022
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farah
@aladwan2003 (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 11, 2022
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Jazz Ghataora, PhD
@JazzSynBio (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 11, 2022
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Phages.fr
@Phages_fr (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 11, 2022
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Richard Allen White III, PhD
@raw9371 (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Devi Prasanna Dash
@dashdp123 (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Gavin H Thomas
@GavinHThomas (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Jemal Ali
@jemal14 (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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The Uyaguari laboratory at UoM
@MiguelUyaguari1 (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Simon Roux
@simroux_virus (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Andrew millard
@milja001 (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Darren N. Nesbeth
@GenePlumber (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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@vic_bioinfo@genomic.social
@Vic_Bioinfo (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Russel Vincent
@RusselVincent25 (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Invictus Samael
@InvictusSamael (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Lingchong You
@lingchongyou (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Sahal Sabilil Muttaqin
@SahalSabilil (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Tom Ellis
@ProfTomEllis (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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Maxuel Andrade
@andrade_maxuel (Twitter)RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
view full postJune 10, 2022
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ACS Synthetic Bio
@ACSSynBio (Twitter)Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragments in a single round, as shown with the 40 kb T7 bacteriophage genome. The article shares protocols and design principles: https://t.co/iyrTqjwphd https://t.co/Lr12ej7nlQ
view full postJune 10, 2022
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Robyn Hall
@Virologica (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postJune 3, 2022
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synbiopapers
@synbio_papers (Twitter)Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/wngym0tXGA https://t.co/IRiNgyO2Te
view full postJune 3, 2022
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Afif Pranaya Jati
@afifpj (Twitter)RT @synbio_papers: [ASAP] Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/MGbq4LXLkG
view full postJune 3, 2022
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Akos Nyerges
@AkosNyerges (Twitter)RT @synbio_papers: [ASAP] Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/MGbq4LXLkG
view full postJune 2, 2022
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Alex Prima
@s4337109 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postJune 1, 2022
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New England Biolabs
@NEBiolabs (Twitter)RT @TwistBioscience: New work from @NEBiolabs researchers- Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly De…
view full postJune 1, 2022
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Twist Bioscience
@TwistBioscience (Twitter)New work from @NEBiolabs researchers- Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design. #synbio @ACSPublications Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design | Synthetic Biology https://t.co/ODBWKskF5L
view full postJune 1, 2022
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Félix de Carpentier
@FlixdeCarpenti1 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 31, 2022
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Tom Dongmin Kim @tomdongmin.bsky.social
@TomDongmin (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 31, 2022
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Geetha Saarunya, PhD
@DrSaarunya (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 31, 2022
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Talal Hossain
@talal_hossain (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Thuan Phu NGUYEN-VO
@thuanpnv (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Daisuke Inoue @VRchat: Benthos
@DInoueMT (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Sebastian Robalino Espinosa
@sebastianRobal4 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Assala Samad
@assalasamad (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Franklin Nobrega
@NobregaFL (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Shanmuga_Sozhamannan@AuSpread
@AuSpread (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Christine Mordstein
@CMordstein (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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José M. González
@jmglezh (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Rob Hurt
@robertchurt (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Atif Khan
@Atif_micro (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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D_•
@_xuwen (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Eduardo Quiroz Guzmán
@EduardoQG77 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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SpeedyMicrobesSYNBIO
@MicrobesSpeedy (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Rafa Pinilla-Redondo
@rafapinilla92 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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zeyad almajed
@zeyad_M_almajed (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Russel Vincent
@RusselVincent25 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Invictus Samael
@InvictusSamael (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Afif Pranaya Jati
@afifpj (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Jazz Ghataora, PhD
@JazzSynBio (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Tominaga K. (tomiken)
@pacyc1841 (Twitter)Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design | ACS Synthetic Biology https://t.co/71M1PTKHmK
view full postMay 30, 2022
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rizkipradipta
@rizkipradipta (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Sahal Sabilil Muttaqin
@SahalSabilil (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Sebastian Leptihn
@LeptihnLab (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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WeiTing Lin(GR15
@weitinglin66 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Andrew millard
@milja001 (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Vasili Hauryliuk
@hauryliuk (Twitter)RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Olivier Borkowski
@O_Borkowski (Twitter)Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
view full postMay 30, 2022
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Scott_GK_MacLeod_WUaS_worlduniversityandschool.org
@scottmacleod (Twitter)RT @NEBiolabs: @NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recove…
view full postMay 27, 2022
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ろばろば@幹細胞培養わからん
@kororoad1 (Twitter)RT @NEBiolabs: @NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recove…
view full postMay 27, 2022
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New England Biolabs
@NEBiolabs (Twitter)@NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recovering infectious phage particles after transformation! These protocols can be applied to rapidly engineer a wide variety of targets. https://t.co/flRaLsYEby
view full postMay 27, 2022
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GeneConstructor
@GeneConstructor (Twitter)RT @synbio_papers: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design: Large DNA constructs (>10 kb) are…
view full postMay 26, 2022
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synbiopapers
@synbio_papers (Twitter)Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design: Large DNA constructs (>10 kb) are invaluable tools for genetic engineering and the development of therapeutics. However, the manufacture of these constructs is… https://t.co/D1CgMUX1HU https://t.co/5t6Jd0EQBp
view full postMay 25, 2022
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Phage Papers
@Phage_Papers (Twitter)Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/7FA72yi9Jl
view full postMay 25, 2022
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view full postDecember 14, 2025
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doi:10.1021/acssynbio.1c00525. Jin, F. J., Wang, B. T., Wang, Z. D., Jin, L., and Han, P. (2022). CRISPR/Cas9-Based genome editing and its application in ...
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https://doi.org/10.1021/acssynbio.1c00525. Pryor, J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020) ...
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Abstract Synopsis
- The study explores using Golden Gate assembly (GGA), a DNA construction method, to efficiently create large DNA constructs, specifically demonstrating the assembly of a 40 kb T7 bacteriophage genome from up to 52 DNA parts in a single reaction.
- Traditionally, constructing large DNA sequences involves multiple complex steps, but this research shows that with optimized protocols, it's possible to assemble many fragments in one go, saving time and effort.
- The successful assembly and recovery of infectious phage particles suggest that these optimized assembly protocols can be applied broadly to quickly engineer large and complex DNA constructs for various scientific and therapeutic purposes.
Calin Plesa
@calinplesa (Twitter)