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The discussions reflect enthusiasm and recognition of the method’s potential, referencing specific achievements like assembling a 40 kb genome from 52 parts and noting protocols for large DNA construct assembly. Language such as 'optimized,' 'rapid,' and 'viable phage' convey excitement about the advancement's significance and practical applications.
Most discussions support the findings, highlighting successful assembly of the large genome and emphasizing its significance.
Discussions show high interest, with numerous posts referencing protocols, optimization, and broad applications of the method.
Posts often delve into technical details and implications, indicating moderate engagement and curiosity about the techniques.
The consensus underscores the high impact of this work on synthetic biology and genetic engineering, positioning it as a breakthrough.
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragments in a single round, as shown with the 40 kb T7 bacteriophage genome. The article shares protocols and design principles: https://t.co/iyrTqjwphd https://t.co/Lr12ej7nlQ
New work from @NEBiolabs researchers- Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design. #synbio @ACSPublications Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design | Synthetic Biology https://t.co/ODBWKskF5L
@NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble and transform the 40kb T7 phage genome, producing viable phage, and can be applied to rapidly engineer large and complex targets. https://t.co/be3tpRE4JV
@NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recovering infectious phage particles after transformation! These protocols can be applied to rapidly engineer a wide variety of targets. https://t.co/flRaLsYEby
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design: Large DNA constructs (>10 kb) are invaluable tools for genetic engineering and the development of therapeutics. However, the manufacture of these constructs is… https://t.co/D1CgMUX1HU https://t.co/5t6Jd0EQBp
This video discusses using Golden Gate assembly to create large DNA constructs, demonstrating the assembly of a 40 kb bacteriophage genome from 52 DNA parts in a single reaction, highlighting the method's efficiency and…
What is the consensus on Golden Gate Assembly: cycling between 16C and 37C, or only 37C? @NEBiolabs's pub: https://t.co/MCJr1ix0Kh suggests 37C only is superior but in our hands this doesn't seem to be the case. https://t.co/r2PJ6nlz5b
RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
I guess we can finally acknowledge the feasibility to assemble large fragments in vitro, even if one chose not to GGA it with 52 fragments all at once. https://t.co/eXt4DSJHnS
RT @NEBiolabs: @NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble…
@NEBiolabs scientists have optimized Golden Gate Assembly to join >50 DNA fragments at once. The method was used to assemble and transform the 40kb T7 phage genome, producing viable phage, and can be applied to rapidly engineer large and complex targets. https://t.co/be3tpRE4JV
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
RT @ACSSynBio: Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragm…
Testing the boundaries of golden gate assembly: Pryor et al @NEBiolabs find optimization permits assembly of >50 DNA fragments in a single round, as shown with the 40 kb T7 bacteriophage genome. The article shares protocols and design principles: https://t.co/iyrTqjwphd https://t.co/Lr12ej7nlQ
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/wngym0tXGA https://t.co/IRiNgyO2Te
RT @synbio_papers: [ASAP] Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/MGbq4LXLkG
RT @synbio_papers: [ASAP] Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/MGbq4LXLkG
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @TwistBioscience: New work from @NEBiolabs researchers- Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly De…
New work from @NEBiolabs researchers- Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design. #synbio @ACSPublications Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design | Synthetic Biology https://t.co/ODBWKskF5L
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design | ACS Synthetic Biology https://t.co/71M1PTKHmK
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @O_Borkowski: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/2cgnU2hPl7
RT @NEBiolabs: @NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recove…
RT @NEBiolabs: @NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recove…
@NEBiolabs scientists successfully assembled 40 kb T7 bacteriophage genome from 52 parts using Golden Gate Assembly – recovering infectious phage particles after transformation! These protocols can be applied to rapidly engineer a wide variety of targets. https://t.co/flRaLsYEby
RT @synbio_papers: Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design: Large DNA constructs (>10 kb) are…
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design: Large DNA constructs (>10 kb) are invaluable tools for genetic engineering and the development of therapeutics. However, the manufacture of these constructs is… https://t.co/D1CgMUX1HU https://t.co/5t6Jd0EQBp
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design https://t.co/7FA72yi9Jl
It is worth noting that, so far, the 2017, 6(2), 211–216, DOI: 10.1021/acssynbio. ... 1c00525. report Ebola and Lassa virus cases in real-time, Nat. 34 ...
doi:10.1021/acssynbio.1c00525. Jin, F. J., Wang, B. T., Wang, Z. D., Jin, L., and Han, P. (2022). CRISPR/Cas9-Based genome editing and its application in ...
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https://doi.org/10.1021/acssynbio.1c00525. Pryor, J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020) ...
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