Synopsis of Social media discussions
The discussions include positive remarks about the publication, such as mentioning 'standardize everything for MoClo' and praising visual animations, which reflect genuine interest. The tone varies from casual humor to technical appreciation, showing moderate engagement and acknowledgment of the method's significance in molecular biology.
Agreement
Moderate agreementMost discussions express approval or interest in the publication, with some enthusiastic comments about its utility.
Interest
Moderate level of interestThe chatter demonstrates moderate curiosity, with users mentioning the technique's potential and practical applications.
Engagement
Moderate level of engagementParticipants reference specific methods like MoClo and genetic assembly standards, indicating they are engaging with the technical content.
Impact
Moderate level of impactUsers seem to recognize the method's potential to influence DNA assembly practices, suggesting a perceived moderate impact.
Social Mentions
YouTube
2 Videos
13 Posts
Metrics
Video Views
386
Total Likes
24
Extended Reach
168,295
Social Features
15
Timeline: Posts about article
Top Social Media Posts
Ah-Ram Kim
Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN
view full post
synbiopapers
Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching: The assembly of DNA parts is a critical aspect of contemporary biological research. Gibson assembly and Golden Gate cloning are two popular options. Here,… https://t.co/5bEl0kpP1t https://t.co/r8xlTAKKzq
view full post
Antonio J Vallecillo, PhD
Simple and efficient modification of Golden gate design standards and parts using oligo stitching. https://t.co/iyfxpNaQ2J
view full post
Yoel Ohayon
Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching
https://t.co/5fkg6pWcqC https://t.co/VAfwzC3Zkw
view full post
Nelke 'Demon Lord' Shilovich
@ATinyGreenCell 1. Become a plasmid e beggar
2. Get a 50 of these bad guys: https://t.co/InydAbGrXu
3. You can now more or less standartize everything for MoClo!
view full post
Posts referencing the article
Efficient DNA Modification Using Oligo Stitching Technique
We created an animation movie to illustrate Jonas De Saeger his work and publication. Oligo stitching combines Gibson assembly with Golden Gate cloning for efficient DNA modification, assembly, and editing, offering a flexible tool for molecular biologists.
September 21, 2022
68 views
-
@ATinyGreenCell 1. Become a plasmid e beggar 2. Get a 50 of these bad guys: https://t.co/InydAbGrXu 3. You can now more or less standartize everything for MoClo!
view full postJuly 12, 2023
1
-
ScienceStop
@B0tSci (Twitter)RT @JambenSloom: Nice animation on the latest @GenomePlant "Oligo stitching" publication: https://t.co/j1oDzkytkv https://t.co/U6hbVWy9aH
view full postJuly 1, 2022
1
-
Jonas Blomme
@JambenSloom (Twitter)Nice animation on the latest @GenomePlant "Oligo stitching" publication: https://t.co/j1oDzkytkv https://t.co/U6hbVWy9aH
view full postJuly 1, 2022
1
-
Nikolaos Delkis
@nickdelkis (Twitter)RT @synbio_papers: Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching: The assembly of DNA p…
view full postJune 9, 2022
1
-
Antonio J Vallecillo, PhD
@Vallecillo_AJ (Twitter)Simple and efficient modification of Golden gate design standards and parts using oligo stitching. https://t.co/iyfxpNaQ2J
view full postJune 8, 2022
3
-
synbiopapers
@synbio_papers (Twitter)Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching: The assembly of DNA parts is a critical aspect of contemporary biological research. Gibson assembly and Golden Gate cloning are two popular options. Here,… https://t.co/5bEl0kpP1t https://t.co/r8xlTAKKzq
view full postJune 8, 2022
4
1
-
Yoel Ohayon
@DNA_Nanotech (Twitter)Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching https://t.co/5fkg6pWcqC https://t.co/VAfwzC3Zkw
view full postJune 8, 2022
1
-
WeiTing Lin(GR15
@weitinglin66 (Twitter)RT @ahramkim1128: Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN
view full postFebruary 14, 2022
3
-
Vivek Mutalik
@vivek_mutalik (Twitter)RT @ahramkim1128: Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN
view full postFebruary 14, 2022
3
-
Olivier Borkowski
@O_Borkowski (Twitter)RT @ahramkim1128: Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN
view full postFebruary 14, 2022
3
-
Ah-Ram Kim
@ahramkim1128 (Twitter)Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN
view full postFebruary 11, 2022
8
3
-
bioRxiv Synthetic Biology
@bioRxiv_synbio (Twitter)Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/mFj9AQcvaA #biorxiv_synbio
view full postFebruary 10, 2022
-
bioRxiv
@biorxivpreprint (Twitter)Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/xO6ywWvqom #bioRxiv
view full postFebruary 10, 2022
Abstract Synopsis
- Oligo stitching combines Gibson assembly with Golden Gate cloning, allowing for efficient modification of DNA parts, such as converting parts between standards or assembling sequences without PCR.
- The method can also remove restriction sites from plasmids and facilitate saturation mutagenesis, making it a flexible tool for various genetic design tasks.
- Its simplicity and high efficiency suggest that oligo stitching could become a valuable technique for molecular biologists working on DNA assembly and editing.]
Nelke 'Demon Lord' Shilovich
@nelke_IGB (Twitter)