ACS Synth Biol June 2022 PMID 35675166

Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching.

Jonas De Saeger, Mattias Vermeersch, Christophe Gaillochet, Thomas B Jacobs

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Peak: 6 in Feb 2022

across 2 sources since Feb 2022

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168,295

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386

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Feb 2022 – Jul 2023

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AI synopsis

What people are saying

The discussions include positive remarks about the publication, such as mentioning 'standardize everything for MoClo' and praising visual animations, which reflect genuine interest. The tone varies from casual humor to technical appreciation, showing moderate engagement and acknowledgment of the method's significance in molecular biology.

AgreementModerate agreement

Most discussions express approval or interest in the publication, with some enthusiastic comments about its utility.

InterestModerate interest

The chatter demonstrates moderate curiosity, with users mentioning the technique's potential and practical applications.

EngagementModerate engagement

Participants reference specific methods like MoClo and genetic assembly standards, indicating they are engaging with the technical content.

ImpactModerate impact

Users seem to recognize the method's potential to influence DNA assembly practices, suggesting a perceived moderate impact.

Abstract in brief

Oligo stitching combines Gibson assembly with Golden Gate cloning, allowing for efficient modification of DNA parts, such as converting parts between standards or assembling sequences without PCR.
The method can also remove restriction sites from plasmids and facilitate saturation mutagenesis, making it a flexible tool for various genetic design tasks.
Its simplicity and high efficiency suggest that oligo stitching could become a valuable technique for molecular biologists working on DNA assembly and editing.]

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All mentions

Oligo Stitching for DNA Assembly and Editing Efficiency

Thomas Jacobs · Jun 17, 2022 318 views

Efficient DNA Modification Using Oligo Stitching Technique

We created an animation movie to illustrate Jonas De Saeger his work and publication. Oligo stitching combines Gibson assembly with Golden Gate cloning for efficient DNA modification, assembly, and editing, offering a…

Art2Learn · Sep 21, 2022 68 views

Nelke 'Demon Lord' Shilovich

@nelke_IGB · X

@ATinyGreenCell 1. Become a plasmid e beggar 2. Get a 50 of these bad guys: https://t.co/InydAbGrXu 3. You can now more or less standartize everything for MoClo!

Jul 12, 2023 · 1 like · 1,117 followers View post →

ScienceStop

@B0tSci · X

RT @JambenSloom: Nice animation on the latest @GenomePlant "Oligo stitching" publication: https://t.co/j1oDzkytkv https://t.co/U6hbVWy9aH

Jul 1, 2022 · 1 share · 4,067 followers View post →

Jonas Blomme

@JambenSloom · X

Nice animation on the latest @GenomePlant "Oligo stitching" publication: https://t.co/j1oDzkytkv https://t.co/U6hbVWy9aH

Jul 1, 2022 · 1 share · 515 followers View post →

Nikolaos Delkis

@nickdelkis · X

RT @synbio_papers: Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching: The assembly of DNA p…

Jun 9, 2022 · 1 share · 191 followers View post →

Antonio J Vallecillo, PhD

@Vallecillo_AJ · X

Simple and efficient modification of Golden gate design standards and parts using oligo stitching. https://t.co/iyfxpNaQ2J

Jun 8, 2022 · 3 likes · 530 followers View post →

synbiopapers

@synbio_papers · X

Jun 8, 2022 · 4 likes · 1 share · 4,047 followers View post →

Yoel Ohayon

@DNA_Nanotech · X

Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching https://t.co/5fkg6pWcqC https://t.co/VAfwzC3Zkw

Jun 8, 2022 · 1 like · 3,449 followers View post →

WeiTing Lin(GR15

@weitinglin66 · X

RT @ahramkim1128: Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN

Feb 14, 2022 · 3 shares · 1,290 followers View post →

Vivek Mutalik

@vivek_mutalik · X

RT @ahramkim1128: Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN

Feb 14, 2022 · 3 shares · 5,169 followers View post →

Olivier Borkowski

@O_Borkowski · X

RT @ahramkim1128: Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN

Feb 14, 2022 · 3 shares · 7,107 followers View post →

Ah-Ram Kim

@ahramkim1128 · X

Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/w0JlSkv1jN

Feb 11, 2022 · 8 likes · 3 shares · 282 followers View post →

bioRxiv Synthetic Biology

@bioRxiv_synbio · X

Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/mFj9AQcvaA #biorxiv_synbio

Feb 10, 2022 · 2,032 followers View post →

bioRxiv

@biorxivpreprint · X

Simple and efficient modification of Golden Gate design standards and parts using oligo stitching https://t.co/xO6ywWvqom #bioRxiv

Feb 10, 2022 · 138,082 followers View post →

Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching.

Mentions per month

Where it's discussed

What people are saying

Abstract in brief

Top posts

All mentions

Oligo Stitching for DNA Assembly and Editing Efficiency

Efficient DNA Modification Using Oligo Stitching Technique