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These discussions clearly demonstrate strong agreement that fixation influences the observation of phase separation, with posts highlighting effects such as enhancement and diminishment, using words like 'important' and 'caveat' to convey concern. The tone combines scientific interest with caution, emphasizing methodological impacts and the need for careful interpretation, reflecting high relevance and engagement among the participants.
The discussions uniformly acknowledge that fixation methods, especially PFA, can alter the appearance of phase separation, indicating strong consensus.
Participants show high curiosity about the implications of fixation artifacts, referencing terms like 'important', 'interesting', and discussing mechanisms and cautions.
Most posts delve into specific effects and mechanisms, with some sharing summaries and commentary, reflecting a moderate but meaningful level of engagement.
The conversations underline that the findings significantly influence how scientists interpret cellular behaviors, emphasizing the potential for correcting misinterpretations caused by fixation artifacts.
(1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks to @TjianDarzacq lab for insightful comments and editors and reviewers @eLife for important constructive suggestions. https://t.co/nQVvaTk5xC
Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
Thanks so much to the @eLife team for the nicely written eLife digest summarizing our recent findings that PFA fixation can both enhance and diminish the appearance of LLPS droplets in cells. https://t.co/UNENLywZj3 https://t.co/2k9ZOWeFZX
Important: "Fixation can change the appearance of phase separation in living cells" @eLife https://t.co/WuPinFVg07
"Our work reveals that PFA fixation changes the appearance of LLPS from living cells, presents a caveat in studying LLPS using fixation-based methods, and suggests a mechanism underlying the fixation artifact" https://t.co/UXNc4bT6jv
This video explores how fixation alters the visual interpretation of phase separation in living cells, based on research by IrgenGioro et al. The findings highlight crucial insights into liquid-liquid phase separation…
This video discusses how fixation can change the appearance of phase separation in living cells. It provides insights into the effects of fixation methods on liquid-liquid phase separation, particularly involving…
RT @yoshida_shawn: Specifically, we review the effects of various fixatives on proteins that participate in LLPS (https://t.co/yNVMcw9bl6),…
Specifically, we review the effects of various fixatives on proteins that participate in LLPS (https://t.co/yNVMcw9bl6), bind to chromatin, associate with organelles, etc., and identified different mechanisms that can cause artifacts, depending on the protein. 2/
RT @aemonten: "Our work reveals that PFA fixation changes the appearance of LLPS from living cells, presents a caveat in studying LLPS usin…
RT @aemonten: "Our work reveals that PFA fixation changes the appearance of LLPS from living cells, presents a caveat in studying LLPS usin…
RT @aemonten: "Our work reveals that PFA fixation changes the appearance of LLPS from living cells, presents a caveat in studying LLPS usin…
"Our work reveals that PFA fixation changes the appearance of LLPS from living cells, presents a caveat in studying LLPS using fixation-based methods, and suggests a mechanism underlying the fixation artifact" https://t.co/UXNc4bT6jv
RT @grnfluoresceblg: Important: "Fixation can change the appearance of phase separation in living cells" @eLife https://t.co/WuPinFVg07
RT @grnfluoresceblg: Important: "Fixation can change the appearance of phase separation in living cells" @eLife https://t.co/WuPinFVg07
Important: "Fixation can change the appearance of phase separation in living cells" @eLife https://t.co/WuPinFVg07
RT @schong_ustc: Thanks so much to the @eLife team for the nicely written eLife digest summarizing our recent findings that PFA fixation ca…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: Thanks so much to the @eLife team for the nicely written eLife digest summarizing our recent findings that PFA fixation ca…
Nice, neat work to show PFA effect on the LLPSs. https://t.co/Adr6xbQgtR
Thanks so much to the @eLife team for the nicely written eLife digest summarizing our recent findings that PFA fixation can both enhance and diminish the appearance of LLPS droplets in cells. https://t.co/UNENLywZj3 https://t.co/2k9ZOWeFZX
https://t.co/XZWZsonXgr
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
RT @schong_ustc: (1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks…
(1/3) Our preprint on PFA fixation enhancing and diminishing LLPS appearance in living cells is now published! Many thanks to @TjianDarzacq lab for insightful comments and editors and reviewers @eLife for important constructive suggestions. https://t.co/nQVvaTk5xC
RT @Bunshu23: Cell fixation with paraformaldehyde enhanced and diminished putative liquid-liquid phase separation (LLPS) behaviors, and fix…
Cell fixation with paraformaldehyde enhanced and diminished putative liquid-liquid phase separation (LLPS) behaviors, and fixation-modulator glycine reversed the enhancement effects of fixation, urging caution in the interpretation of LLPS with fixation. https://t.co/UxX4J7eX0v
S. Irgen-Gioro, S. R. Yoshida, V. Walling, S. Chong, Fixation can change the appearance of phase separation in living cells. Elife 11 (2022). https://t.co/0m4Uk1iNCU
RT @biorxiv_cellbio: #PeerReview from @eLife of
RT @biorxiv_cellbio: #PeerReview from @eLife of
#PeerReview from @eLife of
#PeerReview from @eLife of
RT @kerstinbys: Interesting findings - Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/tJfRoYclN9
細胞のPFA固定で液液相分離が促進されたり、逆に解消されたりすることがあるという報告。固定サンプルのみの解析だけだとアーティファクトを拾ってしまう可能性があり危険なのだ。 Fixation Can Change the Appearance of Phase Separation in Living Cells | bioRxiv https://t.co/c04JhhZHH6
RT @biorxivpreprint: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
Interesting findings - Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/tJfRoYclN9
RT @biorxivpreprint: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
RT @biorxivpreprint: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
RT @biorxivpreprint: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
RT @biorxivpreprint: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
RT @biorxivpreprint: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
RT @biorxivpreprint: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv
RT @Pha_Tran_Papers: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
RT @Pha_Tran_Papers: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
RT @Pha_Tran_Papers: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
RT @Pha_Tran_Papers: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
RT @Pha_Tran_Papers: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
RT @Pha_Tran_Papers: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
RT @Pha_Tran_Papers: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/71NYz4WdHl
RT @biorxiv_cellbio: Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/3Jk0bcmcdh #biorxiv_cellbio
Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/3Jk0bcmcdh #biorxiv_cellbio
Fixation Can Change the Appearance of Phase Separation in Living Cells https://t.co/CysMjRqQnk #bioRxiv