Vladimir Nekrasov
@vladnekrasov · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
May 12, 2022 · 7 likes · 3 shares · 554 followers
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Genome Biol January 2023 PMID 36639800
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform.
Mentions
16
Peak: 8 in Jan 2023
across 2 sources since May 2022
Extended reach
151,984
potential audience
Total likes
21
Video views
875
Mentions per month
May 2022 – Feb 2025Where it's discussed
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X / Twitter14 posts
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YouTube2 videos
AI synopsis
What people are saying
Discussions highlight the innovative ITER platform's ability to rapidly optimize gene editing in crops, with phrases like 'high-throughput testing' and 'game-changing' emphasizing excitement. The tone reflects enthusiasm for how this advancement could accelerate plant breeding and improve crop traits, illustrating a strong consensus on its high impact and importance.
AgreementStrong agreement
All discussions are highly positive and supportive, emphasizing the significance of the research and its potential applications.
InterestHigh interest
The conversations demonstrate strong curiosity about the innovative high-throughput platform and its implications for plant genome editing.
EngagementHigh engagement
Participants delve into technical details, collaborations, and future prospects, showcasing deep engagement with the subject.
ImpactHigh impact
The consensus recognizes the research as a breakthrough with wide-ranging impact on agricultural biotechnology and crop development.
Abstract in brief
- The study introduces ITER, a high-throughput platform using 96-well arrayed protoplast transfections and high-content imaging, to rapidly optimize CRISPR gene-editing tools in wheat and maize, enabling a full cycle from design to results in just 3 weeks.
- Researchers used ITER to enhance the activity of LbCas12a-based adenine base editors (ABEs) in plants, achieving a significant increase in editing efficiency from nearly undetectable levels to 40%, and successfully generated base-edited plants with stable inheritance of edits.
- The findings demonstrate that ITER is a versatile and efficient tool for improving genome editing reagents, which can be broadly applied to develop advanced editing systems like mutagenic nucleases and cytosine base editors across diverse plant species.
Top posts
Most liked on X and BlueskyVIB Tech Watch
@VIBTechWatch · X
In the need of high-troughput testing of gene-editing reagents? Check out the ITER pipeline developed by the team of Thomas Jacobs in collab with @BASF, @VIBFlowCore and VIB screening core! https://t.co/ZEqcSM4sDu
Jan 20, 2023 · 3 likes · 1 share · 2,090 followers
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@COST_PlantEd
@COST_PlantEd · X
-> Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
Jan 15, 2023 · 3 likes · 1 share · 936 followers
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bioRxiv
@biorxivpreprint · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Szfx9XGzud #bioRxiv
May 11, 2022 · 1 like · 136,927 followers
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CRISPRpapers
@CRISPR_papers · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Igvlwi1fL1
Jan 14, 2023 · 3,894 followers
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Penny Hundleby
@sparrow_penny · X
RT @vladnekrasov: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
May 15, 2022 · 3 shares · 2,094 followers
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All mentions
Maize Mesophyll Protoplast Preparation for Genome Editing Analysis
Learn how to prepare Zea mays B104 protoplasts for genome editing studies. The protocol, adapted from Arabidopsis, details protoplast isolation, filtration, and transfection methods for efficient gene editing…
Thomas Jacobs · Jan 26, 2023
816 views
Maize Protoplast Transfection
Thomas Jacobs · Feb 20, 2025
59 views
VIB Flow Core Ghent
@VIBFlowCoreGT · X
RT @VIBTechWatch: In the need of high-troughput testing of gene-editing reagents? Check out the ITER pipeline developed by the team of Tho…
Jan 20, 2023 · 1 share · 1,002 followers
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VIB Tech Watch
@VIBTechWatch · X
In the need of high-troughput testing of gene-editing reagents? Check out the ITER pipeline developed by the team of Thomas Jacobs in collab with @BASF, @VIBFlowCore and VIB screening core! https://t.co/ZEqcSM4sDu
Jan 20, 2023 · 3 likes · 1 share · 2,090 followers
View post →
UGent Bibliography
@ugentbiblio · X
Systematic optimization of Cas12a base editors in wheat and maize using th... https://t.co/eQ6d1NYeEB #WE09
Jan 19, 2023 · 308 followers
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Apriadi Situmorang
@ApriadiSitumor1 · X
RT @COST_PlantEd: -> Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
Jan 16, 2023 · 1 share · 81 followers
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@COST_PlantEd
@COST_PlantEd · X
-> Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
Jan 15, 2023 · 3 likes · 1 share · 936 followers
View post →
CRISPRpapers
@CRISPR_papers · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Igvlwi1fL1
Jan 14, 2023 · 3,894 followers
View post →
Plant Genomics
@PlantGenomics · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/cYArLF1VYu https://t.co/DVdyRTZNyr
Jan 14, 2023 · 274 followers
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GeetikaBhandari(SugarPlum)
@geetikabhandari · X
RT @vladnekrasov: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
May 16, 2022 · 3 shares · 261 followers
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Penny Hundleby
@sparrow_penny · X
RT @vladnekrasov: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
May 15, 2022 · 3 shares · 2,094 followers
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Florian Hahn
@fl_hahn · X
RT @vladnekrasov: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
May 12, 2022 · 3 shares · 625 followers
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Vladimir Nekrasov
@vladnekrasov · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
May 12, 2022 · 7 likes · 3 shares · 554 followers
View post →
Engineering Papers
@eng_papers · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/r831i9ToUO
May 11, 2022 · 45 followers
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bioRxiv Synthetic Biology
@bioRxiv_synbio · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Uzxdi0XJCM #biorxiv_synbio
May 11, 2022 · 1,987 followers
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bioRxiv
@biorxivpreprint · X
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Szfx9XGzud #bioRxiv
May 11, 2022 · 1 like · 136,927 followers
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