Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform.

Christophe Gaillochet, Alexandra Peña Fernández, Vera Goossens, Katelijn D'Halluin, Andrzej Drozdzecki, Myriam Shafie, Julie Van Duyse, Gert Van Isterdael, Camila Gonzalez, Mattias Vermeersch, Jonas De Saeger, Ward Develtere, Dominique Audenaert, David De Vleesschauwer, Frank Meulewaeter

January 2023 Genome Biol

Synopsis of Social media discussions

Discussions highlight the innovative ITER platform's ability to rapidly optimize gene editing in crops, with phrases like 'high-throughput testing' and 'game-changing' emphasizing excitement. The tone reflects enthusiasm for how this advancement could accelerate plant breeding and improve crop traits, illustrating a strong consensus on its high impact and importance.

A
Agreement
 Strong agreement

All discussions are highly positive and supportive, emphasizing the significance of the research and its potential applications.

I
Interest
 High level of interest

The conversations demonstrate strong curiosity about the innovative high-throughput platform and its implications for plant genome editing.

E
Engagement
 High engagement

Participants delve into technical details, collaborations, and future prospects, showcasing deep engagement with the subject.

I
Impact
 High level of impact

The consensus recognizes the research as a breakthrough with wide-ranging impact on agricultural biotechnology and crop development.

Social Mentions

YouTube

2 Videos

Twitter

14 Posts

Metrics

Video Views

875

Total Likes

21

Extended Reach

151,984

Social Features

16

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Maize Mesophyll Protoplast Preparation for Genome Editing Analysis

Maize Mesophyll Protoplast Preparation for Genome Editing Analysis

Learn how to prepare Zea mays B104 protoplasts for genome editing studies. The protocol, adapted from Arabidopsis, details protoplast isolation, filtration, and transfection methods for efficient gene editing applications.

Thomas Jacobs

January 26, 2023

816 views

Thomas Jacobs

February 20, 2025

59 views

  • VIB Flow Core Ghent
    @VIBFlowCoreGT (Twitter)

    RT @VIBTechWatch: In the need of high-troughput testing of gene-editing reagents? Check out the ITER pipeline developed by the team of Tho…
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    January 20, 2023

    1

  • VIB Tech Watch
    @VIBTechWatch (Twitter)

    In the need of high-troughput testing of gene-editing reagents? Check out the ITER pipeline developed by the team of Thomas Jacobs in collab with @BASF, @VIBFlowCore and VIB screening core! https://t.co/ZEqcSM4sDu
    view full post

    January 20, 2023

    3

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  • UGent Bibliography
    @ugentbiblio (Twitter)

    Systematic optimization of Cas12a base editors in wheat and maize using th... https://t.co/eQ6d1NYeEB #WE09
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    January 19, 2023

  • Apriadi Situmorang
    @ApriadiSitumor1 (Twitter)

    RT @COST_PlantEd: -> Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
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    January 16, 2023

    1

  • @COST_PlantEd
    @COST_PlantEd (Twitter)

    -> Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
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    January 15, 2023

    3

    1

  • CRISPRpapers
    @CRISPR_papers (Twitter)

    Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Igvlwi1fL1
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    January 14, 2023

  • Plant Genomics
    @PlantGenomics (Twitter)

    Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/cYArLF1VYu https://t.co/DVdyRTZNyr
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    January 14, 2023

  • GeetikaBhandari(SugarPlum)
    @geetikabhandari (Twitter)

    RT @vladnekrasov: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
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    May 16, 2022

    3

  • Penny Hundleby
    @sparrow_penny (Twitter)

    RT @vladnekrasov: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
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    May 15, 2022

    3

  • Florian Hahn
    @fl_hahn (Twitter)

    RT @vladnekrasov: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
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    May 12, 2022

    3

  • Vladimir Nekrasov
    @vladnekrasov (Twitter)

    Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/P6WTCUG3f1
    view full post

    May 12, 2022

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  • Engineering Papers
    @eng_papers (Twitter)

    Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/r831i9ToUO
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    May 11, 2022

  • bioRxiv Synthetic Biology
    @bioRxiv_synbio (Twitter)

    Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Uzxdi0XJCM #biorxiv_synbio
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    May 11, 2022

  • bioRxiv
    @biorxivpreprint (Twitter)

    Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform https://t.co/Szfx9XGzud #bioRxiv
    view full post

    May 11, 2022

    1

Abstract Synopsis

  • The study introduces ITER, a high-throughput platform using 96-well arrayed protoplast transfections and high-content imaging, to rapidly optimize CRISPR gene-editing tools in wheat and maize, enabling a full cycle from design to results in just 3 weeks.
  • Researchers used ITER to enhance the activity of LbCas12a-based adenine base editors (ABEs) in plants, achieving a significant increase in editing efficiency from nearly undetectable levels to 40%, and successfully generated base-edited plants with stable inheritance of edits.
  • The findings demonstrate that ITER is a versatile and efficient tool for improving genome editing reagents, which can be broadly applied to develop advanced editing systems like mutagenic nucleases and cytosine base editors across diverse plant species.