Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer.

Agathe Couturier, Chloé Virolle, Kelly Goldlust, Annick Berne-Dedieu, Audrey Reuter, Sophie Nolivos, Yoshiharu Yamaichi, Sarah Bigot, Christian Lesterlin

January 2023 Nat Commun

Synopsis of Social media discussions

The discussions highlight admiration for the publication, with phrases like 'Beautiful work' and congratulations indicating recognition of its scientific value. Posts also emphasize the innovative visualization of plasmid transfer, demonstrating interest and curiosity about the methods and findings, though most interactions remain at a surface level rather than deep analysis.

A
Agreement
 Moderate agreement

Most posts express approval and recognize the quality of the work, with comments like 'Beautiful work' and congratulations to the authors, indicating general agreement on its significance.

I
Interest
 High level of interest

Posts show high curiosity and admiration for the real-time visualization techniques, reflecting strong interest in the topic and methodology.

E
Engagement
 Moderate level of engagement

While some posts share enthusiastic reactions and acknowledge the authors, there is limited detailed discussion or critical evaluation, suggesting moderate engagement depth.

I
Impact
 Moderate level of impact

The enthusiasm and recognition from the community hint at a perception that this research could influence the field, but explicit claims about its broader impact are not heavily expressed.

Social Mentions

YouTube

1 Videos

Bluesky

2 Posts

Facebook

2 Posts

Twitter

32 Posts

Blogs

2 Articles

News

4 Articles

Metrics

Video Views

20

Total Likes

108

Extended Reach

593,648

Social Features

43

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Real-time Visualization of Conjugative Plasmid Transfer in Bacteria

Real-time Visualization of Conjugative Plasmid Transfer in Bacteria

This study uses live-cell microscopy to visualize how conjugative plasmids transfer between E. coli bacteria, revealing rapid movement and conversion of DNA, with gene expression regulated by DNA form during transfer.

STEM RTCL TV

April 29, 2023

20 views

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    Real-time visualisation of the intracellular dynamics of Shigella virulence plasmid https://www.biorxiv.org/content/10.1101/2025.07.10.664258v1
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    Real-time visualisation of the intracellular dynamics of Shigella virulence plasmid https://www.biorxiv.org/content/10.1101/2025.07.10.664258v1
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    RT @sbigot2: Happy to share
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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  • Tominaga K. (tomiken)
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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  • Akos Nyerges
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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  • Dr. Melanie Blokesch
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    RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…
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    Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c and coauthors ! Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer https://t.co/m5JuOh5ewv
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Abstract Synopsis

  • This study uses live-cell microscopy to visualize how conjugative plasmids transfer between E. coli bacteria in real time, revealing that the process involves the rapid and efficient movement of plasmid DNA in single-stranded form, which is then quickly converted into double-stranded DNA.
  • The conversion from ssDNA to dsDNA controls when certain plasmid genes are expressed, with early genes being transcribed right after entry of the ssDNA into the recipient cell, and later genes becoming active once the DNA is double-stranded.
  • This temporal regulation ensures that proteins involved in plasmid transfer and maintenance are produced in a specific order, helping the plasmid spread and persist within bacterial populations.]