Nat Commun January 2023 PMID 36653393

Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer.

Agathe Couturier, Chloé Virolle, Kelly Goldlust, Annick Berne-Dedieu, Audrey Reuter, Sophie Nolivos, Yoshiharu Yamaichi, Sarah Bigot, Christian Lesterlin

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Peak: 29 in Jan 2023

across 6 sources since Jan 2023

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What people are saying

The discussions highlight admiration for the publication, with phrases like 'Beautiful work' and congratulations indicating recognition of its scientific value. Posts also emphasize the innovative visualization of plasmid transfer, demonstrating interest and curiosity about the methods and findings, though most interactions remain at a surface level rather than deep analysis.

AgreementModerate agreement

Most posts express approval and recognize the quality of the work, with comments like 'Beautiful work' and congratulations to the authors, indicating general agreement on its significance.

InterestHigh interest

Posts show high curiosity and admiration for the real-time visualization techniques, reflecting strong interest in the topic and methodology.

EngagementModerate engagement

While some posts share enthusiastic reactions and acknowledge the authors, there is limited detailed discussion or critical evaluation, suggesting moderate engagement depth.

ImpactModerate impact

The enthusiasm and recognition from the community hint at a perception that this research could influence the field, but explicit claims about its broader impact are not heavily expressed.

Abstract in brief

This study uses live-cell microscopy to visualize how conjugative plasmids transfer between E. coli bacteria in real time, revealing that the process involves the rapid and efficient movement of plasmid DNA in single-stranded form, which is then quickly converted into double-stranded DNA.
The conversion from ssDNA to dsDNA controls when certain plasmid genes are expressed, with early genes being transcribed right after entry of the ssDNA into the recipient cell, and later genes becoming active once the DNA is double-stranded.
This temporal regulation ensures that proteins involved in plasmid transfer and maintenance are produced in a specific order, helping the plasmid spread and persist within bacterial populations.]

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Real-time Visualization of Conjugative Plasmid Transfer in Bacteria

This study uses live-cell microscopy to visualize how conjugative plasmids transfer between E. coli bacteria, revealing rapid movement and conversion of DNA, with gene expression regulated by DNA form during transfer.

STEM RTCL TV · Apr 29, 2023 20 views

bioRxiv Molecular Biology

@biorxiv-molbio.bsky.social · Bluesky

Real-time visualisation of the intracellular dynamics of Shigella virulence plasmid https://www.biorxiv.org/content/10.1101/2025.07.10.664258v1

Jul 13, 2025 View post →

bioRxivpreprint

@biorxivpreprint.bsky.social · Bluesky

Real-time visualisation of the intracellular dynamics of Shigella virulence plasmid https://www.biorxiv.org/content/10.1101/2025.07.10.664258v1

Jul 13, 2025 View post →

Nature Communications

@NatureComms · X

RT @sbigot2: Happy to share

Mar 24, 2023 · 10 shares · 248,490 followers View post →

SIAM Activity Group on Dynamical Systems

@DynamicsSIAM · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Mar 23, 2023 · 13 shares · 10,903 followers View post →

Nature Communications

@NatureComms · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Mar 23, 2023 · 13 shares · 248,490 followers View post →

Tominaga K. (tomiken)

@pacyc1841 · X

» Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer | Nature Communications https://t.co/KYTJXHpQar

Jan 25, 2023 · 3 likes · 855 followers View post →

Mathilde Guzzo

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RT @sbigot2: Happy to share

Jan 24, 2023 · 10 shares · 1,003 followers View post →

Marie-Eve VAL

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RT @sbigot2: Happy to share

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Canaan Lab

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Rubén de Dios

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RT @sbigot2: Happy to share

Jan 20, 2023 · 10 shares · 275 followers View post →

Koji Yahara

@KojiYahara · X

RT @hlcao: Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer | Nature Communications https://t.co/pofPE…

Jan 20, 2023 · 1 share · 296 followers View post →

Vidya C Darbari

@vidyadarbari · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 20, 2023 · 13 shares · 238 followers View post →

Carlos Blondel

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RT @sbigot2: Happy to share

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Pierre Garcia

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Zeynep Baharoglu

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RT @sbigot2: Happy to share

Jan 20, 2023 · 10 shares · 1,016 followers View post →

sarah bigot

@sbigot2 · X

Happy to share

Jan 20, 2023 · 51 likes · 10 shares · 176 followers View post →

Javier DelaFuente

@DelaFuente_Jav · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 20, 2023 · 13 shares · 678 followers View post →

Corentin Jaboulay

@CJab_Research · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 20, 2023 · 13 shares · 184 followers View post →

Vega Shah

@dr_alphalyrae · X

Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer | Nature Communications https://t.co/MJBcrfrg2y

Jan 20, 2023 · 6 likes · 1 share · 18,713 followers View post →

Toyota_Group_Komaba

@KomaToug · X

RT @DNA_Nanotech: Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer https://t.co/Umlshx2Sxo https://t.c…

Jan 19, 2023 · 2 shares · 669 followers View post →

Franklin Nobrega

@NobregaFL · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 2,418 followers View post →

Cristina Alvarez-Martinez

@Cristin33098502 · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 159 followers View post →

Huiluo Cao 曹慧荦

@hlcao · X

Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer | Nature Communications https://t.co/pofPEdMnEk

Jan 19, 2023 · 1 like · 1 share · 2,827 followers View post →

Zamin Iqbal

@ZaminIqbal · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 9,491 followers View post →

Chloé Virolle

@VirolleChloe · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 137 followers View post →

Goldlust Kelly

@GoldlustKelly · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 270 followers View post →

Akos T. Kovacs

@EvolvedBiofilm · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 21,024 followers View post →

Akos Nyerges

@AkosNyerges · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 2,243 followers View post →

Dr. Melanie Blokesch

@MBlokesch · X

RT @CascalesLab: Beautiful work from the Lesterlin's lab. Congratulations to @CouturierAgath @VirolleChloe @GoldlustKelly @sbigot2, @tacc_c…

Jan 19, 2023 · 13 shares · 6,781 followers View post →

Cascales Lab

@CascalesLab · X

Jan 19, 2023 · 44 likes · 13 shares · 4,562 followers View post →

Yoel Ohayon

@DNA_Nanotech · X

Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer https://t.co/Umlshx2Sxo https://t.co/T57xWCxDcf

Jan 19, 2023 · 3 likes · 2 shares · 3,447 followers View post →

micropapers

@micro_papers · X

Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer https://t.co/rcIN1xiwkz

Jan 19, 2023 · 332 followers View post →

Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer.

Mentions per month

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Abstract in brief

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All mentions

Real-time Visualization of Conjugative Plasmid Transfer in Bacteria