Nat Biotechnol September 2023 PMID 36690762

Precise transcript targeting by CRISPR-Csm complexes.

David Colognori, Marena Trinidad, Jennifer A Doudna

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What people are saying

Many discussions emphasize the high efficiency of RNA knockdown achieved by the CRISPR-Csm complex, with users noting its superiority over existing methods. Phrases like 'really cool work' and 'looks very promising' reflect excitement and positivity, contributing to a tone of enthusiasm around the article. Additionally, references to developers of CRISPR technology highlight respect and recognition for past advancements in the field.

AgreementModerate agreement

Most discussions indicate a supportive view of the publication's findings, highlighting the innovative aspects of the CRISPR-Csm complex.

InterestHigh interest

The high level of curiosity about RNA targeting technologies is evident, signaling a strong interest in the advancements presented.

EngagementModerate engagement

While discussions reference specific elements of the research, the comments tend to remain on the surface without deep analysis or critique.

ImpactHigh impact

The consensus is that this research represents a significant advancement with real potential applications in gene editing and live-cell imaging.

Abstract in brief

Researchers demonstrate that the CRISPR-Csm complex effectively targets and degrades RNA in mammalian cells, overcoming inefficiencies of current methods.
This system allows for high-efficiency RNA knockdown (90-99%) while minimizing off-target effects, outperforming existing techniques like Cas13.
The study also reveals that a catalytically inactive version of Csm can bind RNA specifically, enabling new possibilities for live-cell RNA imaging.

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CRISPR-Csm Complex: Innovations in RNA Targeting Techniques

This video discusses the CRISPR-Csm complex and its ability to target and degrade RNA in mammalian cells, achieving high-efficiency RNA knockdown while minimizing off-target effects. This innovative approach outperforms…

Kelly Pum · Feb 20, 2024 59 views

CRISPR-Csm Complexes: Revolutionizing RNA Targeting Techniques

This video discusses the CRISPR-Csm complex's ability to effectively target and degrade RNA in mammalian cells. Achieving a 90-99% RNA knockdown rate, it minimizes off-target effects and outperforms existing methods…

Samuel Jansen · Feb 24, 2023 29 views

biotechrx

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RT @TXTL_com: Precise transcript targeting by CRISPR-Csm complexes | Nature Biotechnology #TranscriptionAndTranslation #CRISPR https://t.c…

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MEDNTD

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Precise transcript targeting by CRISPR-Csm complexes https://t.co/EckhweRy0R This article find that catalytically inactivated Csm achieves specific and durable RNA binding, a property we harness for live-cell RNA imaging. #KD #RNA #MedEd via @NatureBiotech https://t.co/oYhIWJTerx

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Nature Biotechnology

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Craig D. Mansfield, PhD, EI

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Thamarasee Jeewandara

@Jeew333T · X

I wrote about gene editing advances with a quick shout out to Doudna and Charpentier, joint Chemistry Nobel Laureates & pioneers of #CRISPR - via Precise transcript targeting by CRISPR-Csm complexes for efficient RNA knockdown in human cells

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Hugo Indalecio

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Elisheva

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crazyarchitect.13

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GenBio Inc

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Phys.org Biology

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Phys.org

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MCLovin

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RT @ScienceCrisp: "Precise transcript targeting by #CRISPR-Csm complexes" Colognori D, Trinidad M, Doudna JA. （bioRxiv 2022-06-20) Nat Bio…

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CRISP_SCIENCE

@ScienceCrisp · X

"Precise transcript targeting by #CRISPR-Csm complexes" Colognori D, Trinidad M, Doudna JA. （bioRxiv 2022-06-20) Nat Biotechnol. 2023-01-23. https://t.co/cAYSk42PLn https://t.co/SyD7ugOTcK

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RT @vladnekrasov: This still needs to be tested in plants but looks very promising. Precise transcript targeting by CRISPR-Csm complexes…

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Youdiil Ophinni

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RT @DrLeoRios: Really cool work from @doudna_lab @NatureBiotech #synbio New #CRISPR tool to find and degrade target RNA molecules without…

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CRISPR Articles

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Chenxin Li, PhD (@chenxinli2.bsky.social)

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john morley

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Colognori, D., Trinidad, M. & Doudna, J.A. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol (2023). https://t.co/fLrVFYwF1R

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This still needs to be tested in plants but looks very promising. Precise transcript targeting by CRISPR-Csm complexes | Nature Biotechnology https://t.co/2lTl1khsAf

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Daniël Warmerdam

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Precise transcript targeting by CRISPR-Csm complexes #CRISPR-Csm complex providing high-efficiency RNA knockdown (>90%) and minimal off-target effects in human cells, thereby outperforming short hairpin RNA- and Cas13-mediated knockdown. https://t.co/ApOy02h45G

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CRISPR-Csm Complex: Innovations in RNA Targeting Techniques

CRISPR-Csm Complexes: Revolutionizing RNA Targeting Techniques