An extensible vector toolkit and parts library for advanced engineering of plant genomes.

James C Chamness, Jitesh Kumar, Anna J Cruz, Elissa Rhuby, Mason J Holum, Jon P Cody, Redeat Tibebu, Maria Elena Gamo, Colby G Starker, Feng Zhang, Daniel F Voytas

June 2023 Plant Genome

Synopsis of Social media discussions

The group demonstrates strong interest in the subject, with users highlighting the paper as a useful resource and expressing a desire for broader characterization of plant parts. The tone is generally positive and forward-looking, reflecting a recognition of its potential to advance plant biotechnology research.

A
Agreement
 Moderate agreement

Most discussions recognize the value of the new toolkit, with some noting it as a promising starting point or a useful resource for plant genome engineering.

I
Interest
 High level of interest

Participants show high curiosity and enthusiasm, evidenced by phrases like 'looking for modular vectors' and expressing a desire for more extensive resources.

E
Engagement
 Moderate level of engagement

Comments include references to related topics like CRISPR and genome editing, indicating a moderate level of involvement, although mainly centered around sharing or endorsing the paper.

I
Impact
 Moderate level of impact

The posts suggest the publication could influence ongoing research practices, especially by providing a flexible toolkit for plant scientists, even if some wish for further expansion.

Social Mentions

YouTube

1 Videos

Twitter

13 Posts

Blogs

2 Articles

Metrics

Video Views

17

Total Likes

40

Extended Reach

171,792

Social Features

16

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Advanced Plant Genome Engineering with Extensible Vector Toolkit

Advanced Plant Genome Engineering with Extensible Vector Toolkit

Plant biotechnology is advancing with new transformation and genome engineering techniques, but designing transformation constructs remains complex due to growing demands for delivery and expression in plant cells. A universal Golden Gate cloning toolkit is introduced that aligns with the Phytobrick standard and allows for

Social RTCL TV

October 8, 2023

17 views

  • René Inckemann
    @rene_inckemann (Twitter)

    @ATinyGreenCell I think there are a few papers on small sets of validated parts, but usually just the common (viral) ones. I like this paper as a starting point: https://t.co/3se3sVwfR2 However I wish there would be a way more extensive list of plant parts, which are fully characterized
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    July 11, 2023

  • Plant Editors
    @PlantEditors (Twitter)

    RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…
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    April 17, 2023

    5

  • Dr. Götz Hensel
    @GoeHensel (Twitter)

    RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…
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    April 17, 2023

    5

  • Plant Genome Editing
    @GEd_Plants (Twitter)

    RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…
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    April 17, 2023

    5

  • The Plant Genome
    @plantgenome (Twitter)

    Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced engineering of plant genomes by #Voytas_lab #CRISPR @kumar2501 @friendzhang @UMNews @NSF @ENERGY @USDA_NIFA https://t.co/cJd0B4R43U https://t.co/w0B1ARmqoH
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    April 17, 2023

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  • Carolina Grandellis
    @cgrandellis (Twitter)

    An extensible vector toolkit and parts library for advanced engineering of plant genomes - PubMed https://t.co/Ln2MCmiQQI
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    March 22, 2023

    1

  • Armando Casas-Mollano
    @Armandomics (Twitter)

    RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…
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    October 24, 2022

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  • Kraemer Lab
    @PlantAdaptation (Twitter)

    RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…
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    October 19, 2022

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  • Maria Voloshina
    @voloshima (Twitter)

    RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…
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    October 19, 2022

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  • Dmitry Lapin
    @laplap10 (Twitter)

    RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…
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    October 18, 2022

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  • Biorxiv Reddit
    @BiorxivReddit (Twitter)

    An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/FzaisxWQ3E
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    October 18, 2022

  • bioRxiv Plant Bio
    @biorxiv_plants (Twitter)

    An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #biorxiv_plants
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    October 18, 2022

    19

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  • bioRxiv
    @biorxivpreprint (Twitter)

    An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/5V7wiG6FPl #bioRxiv
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    October 18, 2022

Abstract Synopsis

  • Plant biotechnology is advancing with new transformation and genome engineering techniques, but designing transformation constructs remains complex due to growing demands for delivery and expression in plant cells.
  • A universal Golden Gate cloning toolkit is introduced that aligns with the Phytobrick standard and allows for easier assembly of complex DNA structures, improving on existing kits in capacity, flexibility, and extensibility.
  • The toolkit includes a library of new Phytobricks with important regulatory elements and coding sequences, and features dual-luciferase assays to study the effects of different genetic components on gene expression, facilitating quicker testing and implementation of new plant engineering tools.