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Plant Genome June 2023 PMID 36896468

An extensible vector toolkit and parts library for advanced engineering of plant genomes.

James C Chamness, Jitesh Kumar, Anna J Cruz, Elissa Rhuby, Mason J Holum, Jon P Cody, Redeat Tibebu, Maria Elena Gamo, Colby G Starker, Feng Zhang, Daniel F Voytas

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Mentions

16
Peak: 7 in Oct 2022
across 3 sources since Oct 2022

Extended reach

171,792
potential audience

Total likes

40

Video views

17

Mentions per month

Oct 2022 – Oct 2023

Where it's discussed

  • X / Twitter13 posts
  • Blogs2 articles
  • YouTube1 videos

AI synopsis

What people are saying

The group demonstrates strong interest in the subject, with users highlighting the paper as a useful resource and expressing a desire for broader characterization of plant parts. The tone is generally positive and forward-looking, reflecting a recognition of its potential to advance plant biotechnology research.

AgreementModerate agreement

Most discussions recognize the value of the new toolkit, with some noting it as a promising starting point or a useful resource for plant genome engineering.

InterestHigh interest

Participants show high curiosity and enthusiasm, evidenced by phrases like 'looking for modular vectors' and expressing a desire for more extensive resources.

EngagementModerate engagement

Comments include references to related topics like CRISPR and genome editing, indicating a moderate level of involvement, although mainly centered around sharing or endorsing the paper.

ImpactModerate impact

The posts suggest the publication could influence ongoing research practices, especially by providing a flexible toolkit for plant scientists, even if some wish for further expansion.

Abstract in brief

  • Plant biotechnology is advancing with new transformation and genome engineering techniques, but designing transformation constructs remains complex due to growing demands for delivery and expression in plant cells.
  • A universal Golden Gate cloning toolkit is introduced that aligns with the Phytobrick standard and allows for easier assembly of complex DNA structures, improving on existing kits in capacity, flexibility, and extensibility.
  • The toolkit includes a library of new Phytobricks with important regulatory elements and coding sequences, and features dual-luciferase assays to study the effects of different genetic components on gene expression, facilitating quicker testing and implementation of new plant engineering tools.
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Top posts

Most liked on X and Bluesky
The Plant Genome
@plantgenome · X

Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced engineering of plant genomes by #Voytas_lab #CRISPR @kumar2501 @friendzhang @UMNews @NSF @ENERGY @USDA_NIFA https://t.co/cJd0B4R43U https://t.co/w0B1ARmqoH

Apr 17, 2023 · 20 likes · 5 shares · 3,498 followers View post →
bioRxiv Plant Bio
@biorxiv_plants · X

An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #biorxiv_plants

Oct 18, 2022 · 19 likes · 4 shares · 15,846 followers View post →
Carolina Grandellis
@cgrandellis · X

An extensible vector toolkit and parts library for advanced engineering of plant genomes - PubMed https://t.co/Ln2MCmiQQI

Mar 22, 2023 · 1 like · 1,055 followers View post →
bioRxiv
@biorxivpreprint · X

An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/5V7wiG6FPl #bioRxiv

Oct 18, 2022 · 136,932 followers View post →
Plant Editors
@PlantEditors · X

RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…

Apr 17, 2023 · 5 shares · 4,519 followers View post →
Dr. Götz Hensel
@GoeHensel · X

RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…

Apr 17, 2023 · 5 shares · 2,975 followers View post →

All mentions

René Inckemann
@rene_inckemann · X

@ATinyGreenCell I think there are a few papers on small sets of validated parts, but usually just the common (viral) ones. I like this paper as a starting point: https://t.co/3se3sVwfR2 However I wish there would be a way more extensive list of plant parts, which are fully characterized

Jul 11, 2023 · 1,713 followers View post →
Plant Editors
@PlantEditors · X

RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…

Apr 17, 2023 · 5 shares · 4,519 followers View post →
Dr. Götz Hensel
@GoeHensel · X

RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…

Apr 17, 2023 · 5 shares · 2,975 followers View post →
Plant Genome Editing
@GEd_Plants · X

RT @plantgenome: Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced enginee…

Apr 17, 2023 · 5 shares · 1,025 followers View post →
The Plant Genome
@plantgenome · X

Looking for modular plant genome editing vectors? Here is extensible vector toolkit and parts library for advanced engineering of plant genomes by #Voytas_lab #CRISPR @kumar2501 @friendzhang @UMNews @NSF @ENERGY @USDA_NIFA https://t.co/cJd0B4R43U https://t.co/w0B1ARmqoH

Apr 17, 2023 · 20 likes · 5 shares · 3,498 followers View post →
Carolina Grandellis
@cgrandellis · X

An extensible vector toolkit and parts library for advanced engineering of plant genomes - PubMed https://t.co/Ln2MCmiQQI

Mar 22, 2023 · 1 like · 1,055 followers View post →
Armando Casas-Mollano
@Armandomics · X

RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…

Oct 24, 2022 · 4 shares · 229 followers View post →
Kraemer Lab
@PlantAdaptation · X

RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…

Oct 19, 2022 · 4 shares · 1,516 followers View post →
Maria Voloshina
@voloshima · X

RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…

Oct 19, 2022 · 4 shares · 87 followers View post →
Dmitry Lapin
@laplap10 · X

RT @biorxiv_plants: An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #bior…

Oct 18, 2022 · 4 shares · 2,262 followers View post →
Biorxiv Reddit
@BiorxivReddit · X

An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/FzaisxWQ3E

Oct 18, 2022 · 39 followers View post →
bioRxiv Plant Bio
@biorxiv_plants · X

An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/V9Wor0eKRW #biorxiv_plants

Oct 18, 2022 · 19 likes · 4 shares · 15,846 followers View post →
bioRxiv
@biorxivpreprint · X

An Extensible Vector Toolkit and Parts Library for Advanced Engineering of Plant Genomes https://t.co/5V7wiG6FPl #bioRxiv

Oct 18, 2022 · 136,932 followers View post →