Nat Commun May 2023 PMID 37149708

High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing.

Zhuo-Xing Shi, Zhi-Chao Chen, Jia-Yong Zhong, Kun-Hua Hu, Ying-Feng Zheng, Ying Chen, Shang-Qian Xie, Xiao-Chen Bo, Feng Luo, Chong Tang, Chuan-Le Xiao, Yi-Zhi Liu

Mentions

16
Peak: 12 in May 2023
across 3 sources since May 2023

Extended reach

27,611
potential audience

Total likes

14

Video views

41

Mentions per month

May 2023 – Aug 2023

Where it's discussed

  • X / Twitter13 posts
  • Blogs2 articles
  • YouTube1 videos

AI synopsis

What people are saying

The collective discussions express high enthusiasm for the method's capabilities, citing phrases like 'high-throughput and high-accuracy' and 'removes artifacts,' which underscore its technical improvements and potential impact. Words like 'reproducibility' and 'practical tool' demonstrate a recognition of its broader scientific significance, contributing to an overall positive and impactful perception.

AgreementModerate agreement

Most discussions highlight the benefits of the new method, emphasizing its high throughput and accuracy, indicating general agreement on its scientific value.

InterestHigh interest

The discussions show strong interest, with many commenters praising the technique's potential and mentioning its relevance to current research challenges.

EngagementModerate engagement

Participants are engaging deeply by discussing technical aspects like artifact removal, concatenation, reproducibility, and applications to transcriptomics, reflecting thoughtful analysis.

ImpactHigh impact

The comments suggest that this advancement could significantly influence future research methodologies and reproducibility standards in single-cell sequencing.

Abstract in brief

  • HIT-scISOseq is a new method that enhances single-cell RNA isoform sequencing by removing artifacts and combining multiple cDNA sequences, leading to higher throughput and accuracy.
  • This technique allows researchers to generate over 10 million accurate long-reads from a single sequencing run, making it more efficient than traditional methods.
  • The method was successfully applied to study 3375 corneal limbus cells, revealing specific gene expression patterns, and offers a practical tool for advancing long-read transcriptomics in research.

Top posts

Most liked on X and Bluesky
RNA-Seq Blog
@RNASeqBlog · X

HIT-scISOseq is a method that removes most artifact #cDNAs and concatenates multiple cDNAs for @PacBio circular consensus sequencing (CCS) to achieve high-throughput and high-accuracy #singlecell #RNA isoform sequencing. https://t.co/maPjkVJLLZ

May 11, 2023 · 7 likes · 2 shares · 8,593 followers View post →
Erik Hom
@erikfyhom · X

High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/QQZGkNe1YO

May 7, 2023 · 4 likes · 2 shares · 684 followers View post →
Long-read metagenomics-Papers
@LR_metagenomics · X

PacBio: High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/hxMEYQihHc

May 7, 2023 · 1 like · 2 shares · 368 followers View post →
Bioinformatics Trends 
@BinfoTrends · X

RT @RNASeqBlog: HIT-scISOseq is a method that removes most artifact #cDNAs and concatenates multiple cDNAs for @PacBio circular consensus s…

May 11, 2023 · 2 shares · 8,159 followers View post →
OpenSci Talk
@OpenSciTalk · X

RT @RobotRrid: RRIDs were included in the Nature Communications paper "High-throughput and high-accuracy single-cell RNA…" (https://t.co/YM…

May 10, 2023 · 1 share · 4,095 followers View post →
Manuel Irimia
@mirimiam · X

RT @erikfyhom: High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/Q…

May 7, 2023 · 2 shares · 2,104 followers View post →

All mentions

Xuan Ye
@xuan_ye · X

RT @LR_metagenomics: PacBio: High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing…

Jun 13, 2023 · 2 shares · 279 followers View post →
Latest on Alternative Splicing
@SplicingNews · X

RT @RNASeqBlog: HIT-scISOseq is a method that removes most artifact #cDNAs and concatenates multiple cDNAs for @PacBio circular consensus s…

May 11, 2023 · 2 shares · 325 followers View post →
Bioinformatics Trends 
@BinfoTrends · X

RT @RNASeqBlog: HIT-scISOseq is a method that removes most artifact #cDNAs and concatenates multiple cDNAs for @PacBio circular consensus s…

May 11, 2023 · 2 shares · 8,159 followers View post →
RNA-Seq Blog
@RNASeqBlog · X

HIT-scISOseq is a method that removes most artifact #cDNAs and concatenates multiple cDNAs for @PacBio circular consensus sequencing (CCS) to achieve high-throughput and high-accuracy #singlecell #RNA isoform sequencing. https://t.co/maPjkVJLLZ

May 11, 2023 · 7 likes · 2 shares · 8,593 followers View post →
OpenSci Talk
@OpenSciTalk · X

RT @RobotRrid: RRIDs were included in the Nature Communications paper "High-throughput and high-accuracy single-cell RNA…" (https://t.co/YM…

May 10, 2023 · 1 share · 4,095 followers View post →
RRID Robot
@RobotRrid · X

RRIDs were included in the Nature Communications paper "High-throughput and high-accuracy single-cell RNA…" (https://t.co/YMjeMkNTaf). RRIDs like this improve reproducibility in scientific research. #methodsmatter #OpenScience

May 10, 2023 · 1 share · 161 followers View post →
Manuel Irimia
@mirimiam · X

RT @erikfyhom: High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/Q…

May 7, 2023 · 2 shares · 2,104 followers View post →
Latest on Alternative Splicing
@SplicingNews · X

RT @erikfyhom: High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/Q…

May 7, 2023 · 2 shares · 325 followers View post →
Erik Hom
@erikfyhom · X

High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/QQZGkNe1YO

May 7, 2023 · 4 likes · 2 shares · 684 followers View post →
Transcriptomes
@transcriptomes · X

High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular cons... https://t.co/v24FAQptZ0

May 7, 2023 · 1,808 followers View post →
Latest on Alternative Splicing
@SplicingNews · X

RT @LR_metagenomics: PacBio: High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing…

May 7, 2023 · 2 shares · 325 followers View post →
single cell papers
@sc_papers · X

High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/fIIDFJ24np https://t.co/knaxVZ54kD

May 7, 2023 · 305 followers View post →
Long-read metagenomics-Papers
@LR_metagenomics · X

PacBio: High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing https://t.co/hxMEYQihHc

May 7, 2023 · 1 like · 2 shares · 368 followers View post →