Removal of AMR plasmids using a mobile, broad host-range CRISPR-Cas9 delivery tool.

David Sünderhauf, Uli Klümper, Elizabeth Pursey, Edze R Westra, William H Gaze, Stineke van Houte

May 2023 Microbiology (Reading)

Synopsis of Social media discussions

Several comments highlight the promising nature of the research, describing it as an 'exciting breakthrough' and 'very cool,' which illustrates enthusiasm for the potential impact of the CRISPR-based tool. The tone varies from curiosity—questioning whether CRISPR will be 'revolutionary'—to optimism about its applications, emphasizing its transformative potential and high relevance in the fight against antimicrobial resistance.

A
Agreement
 Moderate agreement

Most discussions express support or enthusiasm for the research, with comments calling it an 'exciting breakthrough' and 'promising,' indicating general agreement on its significance.

I
Interest
 High level of interest

The posts frequently highlight the novelty and potential of the approach, such as asking if CRISPR will be 'revolutionary' and emphasizing the development of new tools, demonstrating high curiosity and relevance.

E
Engagement
 Moderate level of engagement

Some posts delve into specific details like the broad host-range capability and its implications, reflecting a moderate level of deeper engagement beyond mere acknowledgment.

I
Impact
 High level of impact

Many posts emphasize the potential impact on combating antimicrobial resistance globally, describing the technology as 'very cool' and 'a step towards reducing' resistance, indicating perceived high importance.

Social Mentions

YouTube

2 Videos

Twitter

37 Posts

Blogs

4 Articles

News

24 Articles

Metrics

Video Views

20,946

Total Likes

159

Extended Reach

174,896

Social Features

67

Timeline: Posts about article

Top Social Media Posts

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  • Gilles van Wezel
    @GillesvanWezel (Twitter)

    RT @PaulHoskisson: This is very cool from @davvi36 @UliKluemper @ElliePursey @edzewestra @WillHGaze & @StinekevanHoute Removal of #AMR #p…
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    "Exciting breakthrough in the fight against AMR! Researchers at @UniofExeter have developed a C-Cas9-based plasmid that targets antibiotic-resistant genes, protecting host cells and reversing resistance. A promising step towards reducing global AMR. 10.1099/mic.0.001334 #AMR
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    RT @PaulHoskisson: This is very cool from @davvi36 @UliKluemper @ElliePursey @edzewestra @WillHGaze & @StinekevanHoute Removal of #AMR #p…
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  • José R Penadés
    @jrpenades (Twitter)

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  • Gavin H Thomas
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    This is very cool from @davvi36 @UliKluemper @ElliePursey @edzewestra @WillHGaze & @StinekevanHoute Removal of #AMR #plasmids using a mobile, broad host-range #CRISPR-Cas9 delivery tool https://t.co/JddtT7kNxY #PublishingForTheCommunity @MicrobioSoc
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  • Phys.org Biology
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  • Tue Kjærgaard Nielsen
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Abstract Synopsis

  • The study developed a broad host range CRISPR-Cas9 delivery plasmid, called pKJK5csg, designed to target and remove antimicrobial resistance (AMR) genes carried on plasmids across various bacteria.
  • pKJK5csg was shown to effectively prevent the uptake of AMR plasmids and remove existing resistance plasmids from bacteria, including those from complex microbial communities like pig and human-associated bacteria.
  • This research highlights pKJK5csg as a promising tool for tackling the spread of AMR genes in diverse bacterial populations, potentially helping to reduce antimicrobial resistance in the environment and clinical settings.]