CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression.

Gemma Noviello, Rutger A F Gjaltema, Edda G Schulz

June 2023 Nat Commun

Synopsis of Social media discussions

Discussions highlight the technical advances of CasTuner, with mentions of how the inclusion of RRIDs improves reproducibility, and praise for its ability to finely tune gene expression. The tone reflects both appreciation for the methodology and curiosity about its scientific impact, indicated by phrases like 'improves reproducibility' and 'innovative approach' which underscore the perceived significance.

A
Agreement
 Moderate agreement

Most discussions recognize the publication's technical significance and potential benefits, showing general approval.

I
Interest
 High level of interest

The frequent mentions of terms like 'CasTuner', 'CRISPR', and the emphasis on new toolkit features suggest high curiosity among participants.

E
Engagement
 Moderate level of engagement

Comments cite specific aspects such as reproducibility improvements and methods, indicating a moderate level of detailed engagement.

I
Impact
 Moderate level of impact

Participants acknowledge the importance of the toolkit for advancing gene regulation research, though some focus on technical details rather than broad applications.

Social Mentions

YouTube

1 Videos

Twitter

6 Posts

Metrics

Video Views

29

Total Likes

7

Extended Reach

6,142

Social Features

7

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

CasTuner: Precision Toolkit for Analog Control of Gene Expression

CasTuner: Precision Toolkit for Analog Control of Gene Expression

CasTuner uses CRISPR technology to precisely control protein levels in cells, essential for studying gene dosage effects. It allows fine-tuned, gradual adjustment of gene expression at transcriptional and post-transcriptional levels, enabling more accurate cellular studies.

STEM RTCL TV

September 20, 2023

29 views

  • RRID Robot
    @RobotRrid (Twitter)

    The authors of "CasTuner is a degron and CRISPR/Cas-based toolkit…" (https://t.co/rBvTkeeSDC) included RRIDs in their paper! RRIDs like this improve reproducibility in scientific research. #RRID #STMpublishing
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    June 23, 2023

  • CRISP_SCIENCE
    @ScienceCrisp (Twitter)

    "CasTuner is a degron and #CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression" Noviello G, Gjaltema RAF, Schulz EG. Nat Commun. 2023-06-03. https://t.co/XUMhcc8lIp https://t.co/4NXNRHHBqT
    view full post

    June 10, 2023

    5

  • Europe PMC articles
    @EuropePMC (Twitter)

    New from #ERC_Research https://t.co/ZjOwP7HPNL CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression.
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    June 6, 2023

  • Victor V. Lobanenkov
    @boris_ctcf (Twitter)

    CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. Nat Commun. 2023; 14: 3225. Published online 2023 Jun 3. doi: 10.1038/s41467-023-38909-4 https://t.co/NKcdgoHYcw https://t.co/Fjswhe3Z1G
    view full post

    June 5, 2023

  • CRISPR Articles
    @CRISPR_JC (Twitter)

    CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. https://t.co/kf0zYKJ46M
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    June 4, 2023

  • sexchrpapers
    @sexChr_papers (Twitter)

    CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression https://t.co/v9MvHtRupn
    view full post

    June 4, 2023

    1

Abstract Synopsis

  • CasTuner is a new toolkit that uses CRISPR technology to precisely control the amount of specific proteins in cells, which is important for processes that depend on exact protein levels like gene dosage.
  • It works by deploying Cas protein repressors that can be finely tuned using a small molecule ligand, allowing for gradual adjustment of gene expression either at the transcriptional level (via dCas9 fused to histone deacetylase) or post-transcriptionally (via CasRx targeting RNA).
  • Unlike traditional gene repression methods that turn genes completely off, CasTuner enables smooth, analog control over gene expression, helping researchers study how different protein levels affect cell behavior and gene regulation more accurately.]