Nat Commun June 2023 PMID 37270532

CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression.

Gemma Noviello, Rutger A F Gjaltema, Edda G Schulz

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Peak: 6 in Jun 2023

across 2 sources since Jun 2023

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Jun 2023 – Sep 2023

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AI synopsis

What people are saying

Discussions highlight the technical advances of CasTuner, with mentions of how the inclusion of RRIDs improves reproducibility, and praise for its ability to finely tune gene expression. The tone reflects both appreciation for the methodology and curiosity about its scientific impact, indicated by phrases like 'improves reproducibility' and 'innovative approach' which underscore the perceived significance.

AgreementModerate agreement

Most discussions recognize the publication's technical significance and potential benefits, showing general approval.

InterestHigh interest

The frequent mentions of terms like 'CasTuner', 'CRISPR', and the emphasis on new toolkit features suggest high curiosity among participants.

EngagementModerate engagement

Comments cite specific aspects such as reproducibility improvements and methods, indicating a moderate level of detailed engagement.

ImpactModerate impact

Participants acknowledge the importance of the toolkit for advancing gene regulation research, though some focus on technical details rather than broad applications.

Abstract in brief

CasTuner is a new toolkit that uses CRISPR technology to precisely control the amount of specific proteins in cells, which is important for processes that depend on exact protein levels like gene dosage.
It works by deploying Cas protein repressors that can be finely tuned using a small molecule ligand, allowing for gradual adjustment of gene expression either at the transcriptional level (via dCas9 fused to histone deacetylase) or post-transcriptionally (via CasRx targeting RNA).
Unlike traditional gene repression methods that turn genes completely off, CasTuner enables smooth, analog control over gene expression, helping researchers study how different protein levels affect cell behavior and gene regulation more accurately.]

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All mentions

CasTuner: Precision Toolkit for Analog Control of Gene Expression

CasTuner uses CRISPR technology to precisely control protein levels in cells, essential for studying gene dosage effects. It allows fine-tuned, gradual adjustment of gene expression at transcriptional and…

STEM RTCL TV · Sep 20, 2023 29 views

RRID Robot

@RobotRrid · X

Jun 23, 2023 · 161 followers View post →

CRISP_SCIENCE

@ScienceCrisp · X

Jun 10, 2023 · 5 likes · 1,917 followers View post →

Europe PMC articles

@EuropePMC · X

New from #ERC_Research https://t.co/ZjOwP7HPNL CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression.

Jun 6, 2023 · 1,552 followers View post →

Victor V. Lobanenkov

@boris_ctcf · X

Jun 5, 2023 · 2,046 followers View post →

CRISPR Articles

@CRISPR_JC · X

CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression. https://t.co/kf0zYKJ46M

Jun 4, 2023 · 38 followers View post →

sexchrpapers

@sexChr_papers · X

CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression https://t.co/v9MvHtRupn

Jun 4, 2023 · 1 like · 400 followers View post →