High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors.

Cheng-Kai Shiau, Lina Lu, Rachel Kieser, Kazutaka Fukumura, Timothy Pan, Hsiao-Yun Lin, Jie Yang, Eric L Tong, GaHyun Lee, Yuanqing Yan, Jason T Huse, Ruli Gao

July 2023 Nat Commun

Synopsis of Social media discussions

The discussions demonstrate genuine interest and recognition of the article's importance, exemplified by references to novel techniques like scNanoGPS and mentions of tumor heterogeneity; words like 'innovative' and 'significant' reflect a positive and engaged tone, emphasizing the potential for transformative impact in cancer research.

A
Agreement
 Moderate agreement

Most discussions acknowledge the significance of the new long-read sequencing method, highlighting its innovative analysis of genotypes and phenotypes in tumors.

I
Interest
 High level of interest

Participants show strong interest, with mentions of new tools like scNanoGPS and its potential to advance cancer research.

E
Engagement
 Moderate level of engagement

Posts often include specific references to methods and potential implications, indicating active engagement.

I
Impact
 Moderate level of impact

There's a shared perception that this research could substantially influence future studies in tumor genomics and single-cell analysis.

Social Mentions

YouTube

1 Videos

Twitter

8 Posts

Blogs

2 Articles

News

3 Articles

Metrics

Video Views

57

Total Likes

16

Extended Reach

21,360

Social Features

14

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Single-Cell Long-Read Sequencing for Genotype and Phenotype Analysis in Tumors

Single-Cell Long-Read Sequencing for Genotype and Phenotype Analysis in Tumors

This video discusses the use of single-cell nanopore sequencing to analyze genotypes and phenotypes in human tumors. It highlights the development of scNanoGPS, a tool that processes long-read transcriptomes, enabling detailed insights into gene expression and mutations across thousands of samples.

STEM RTCL TV

October 25, 2023

57 views

  • Ikemen
    @HandsomeKinoko (Twitter)

    High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors @HandsomeKinoko #note https://t.co/3Rp3YHUYDW
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    July 20, 2023

  • Carlos Mora
    @CarlosM49544824 (Twitter)

    RT @BioDecoded: High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors | Nature Co…
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    July 18, 2023

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  • BioDecoded
    @BioDecoded (Twitter)

    High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors | Nature Communications https://t.co/h6s3k4N598 #Bioinformatics https://t.co/0fIrwUmjTp
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    July 17, 2023

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  • Taco Jesse
    @TJesse62 (Twitter)

    RT @RNASeqBlog: Researchers at @NUFeinbergMed have developed scNanoGPS to calculate same-cell #genotypes (#mutations) and #phenotypes (#gen…
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    July 13, 2023

    1

  • RNA-Seq Blog
    @RNASeqBlog (Twitter)

    Researchers at @NUFeinbergMed have developed scNanoGPS to calculate same-cell #genotypes (#mutations) and #phenotypes (#gene/isoform expressions) without short-read nor whitelist guidance. https://t.co/tIrfCwYi1B
    view full post

    July 13, 2023

    3

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  • Nanopore_research
    @Nanoporepapers_ (Twitter)

    High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors Follow us :) https://t.co/Lx2TfpJ2ci
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    July 12, 2023

  • Long-read metagenomics-Papers
    @LR_metagenomics (Twitter)

    nanopore: High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors https://t.co/3YjP8bAETW
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    July 12, 2023

  • Gang Wu
    @wufgang (Twitter)

    High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors https://t.co/77KfSihUF2
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    July 11, 2023

Abstract Synopsis

  • Single-cell nanopore sequencing can enhance multi-omics studies but faces issues like high sequencing errors and reliance on short reads or barcode whitelists.
  • The new tool, scNanoGPS, allows for the analysis of both genotypes and phenotypes in single cells by processing long-read transcriptomes without needing short-read guidance.
  • By using scNanoGPS on thousands of samples, researchers unveiled unique gene expression patterns and mutations in tumor and immune cells, underscoring the diverse roles of various cell types in tumors.