PubHawk
Biochemistry March 2024 PMID 38437583

Discovery of Red-Shifting Mutations in Firefly Luciferase Using High-Throughput Biochemistry.

Clair M Colee, Nicole M Oberlag, Marcell Simon, Owen S Chapman, Lyndsey C Flanagan, Edison S Reid-McLaughlin, Jordan A Gewing-Mullins, Synaida Maiche, Devi F Patel, Andre R O Cavalcanti, Aaron M Leconte

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Mentions

57
Peak: 15 in Nov 2025
across 6 sources since Nov 2023

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Total likes

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Mentions per month

Nov 2023 – Dec 2025

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AI synopsis

What people are saying

The discussions emphasize the importance of the study, noting the discovery of 70 new mutations and congratulating student contributors, with language reflecting excitement and recognition, such as 'new paper,' 'congrats,' and acknowledgment of collaborative efforts. This tone, combined with specific mentions of methodological advances and potential applications, underscores both high interest and moderate perceived impact.

AgreementModerate agreement

Most discussions express positive acknowledgment and support for the significance of the research, especially highlighting the discovery of numerous mutations and the collaborative effort.

InterestHigh interest

The discussions demonstrate high interest, as evidenced by enthusiasm for the novel mutants and appreciation for innovative methods.

EngagementHigh engagement

Participants show deep engagement by highlighting specific mutations, celebrating student contributions, and contextualizing the findings within biochemistry and imaging applications.

ImpactModerate impact

While the shared enthusiasm suggests potential impact, the consensus is that these findings could influence future research and technological applications, but the immediate impact remains moderate.

Abstract in brief

  • This study used high-throughput mutational scanning to identify mutations in firefly luciferase (FLuc) that cause redshifted light emission, which improves deep-tissue imaging.
  • They discovered dozens of new redshifting mutations, notably N229T and T352M, that produce a more consistent emission around 600 nm, supporting the idea of multiple emitters and the role of a water network in the enzyme’s active site.
  • The findings contribute to understanding how protein structure influences emission color, providing valuable data for engineering better bioluminescent probes for imaging purposes.]
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Leconte Research Group
@GroupLeconte · X

New paper from our group! Here, we use a high-throughput approach to identify 70 novel red-shifting mutations of luciferase. Congrats especially to the nine undergraduate student co-authors on the project! https://t.co/uwltVRfI7B

Mar 4, 2024 · 5 likes · 91 followers View post →
ChemRxiv Bio and Med Chem
@chemRxivBiochem · X

Discovery of red-shifting mutations in firefly luciferase using high-throughput biochemistry https://t.co/chui8KmQnR #chemrxiv_biochem

Dec 11, 2023 · 190 followers View post →

All mentions

Leconte Research Group
@GroupLeconte · X

New paper from our group! Here, we use a high-throughput approach to identify 70 novel red-shifting mutations of luciferase. Congrats especially to the nine undergraduate student co-authors on the project! https://t.co/uwltVRfI7B

Mar 4, 2024 · 5 likes · 91 followers View post →
ChemRxiv Bio and Med Chem
@chemRxivBiochem · X

Discovery of red-shifting mutations in firefly luciferase using high-throughput biochemistry https://t.co/chui8KmQnR #chemrxiv_biochem

Dec 11, 2023 · 190 followers View post →
ChemRxiv Bio and Med Chem
@chemRxivBiochem · X

Discovery of red-shifting mutations in firefly luciferase using high-throughput biochemistry https://t.co/viVGRtLT3t #chemrxiv_biochem

Nov 28, 2023 · 190 followers View post →
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