Titer and charge-based heterogeneity multiattribute monitoring of mAbs in cell culture harvest using 2D ProA CEX MS.

Deepika Sarin, Sunil Kumar, Anurag S Rathore

August 2024 Talanta

Synopsis of Social media discussions

Discussions frequently mention how the new 2DLC-MS method offers faster, more efficient analysis with examples like reduced processing time and improved charge variant profiling, reflecting excitement for its potential. The language, including terms like 'game changer' and 'advanced technique,' underscores perceived high impact and interest, while referencing specific research benefits suggests a thoughtful and engaged audience.

A
Agreement
 Moderate agreement

Most discussions acknowledge the publication's promising approach, with comments like 'innovative method' and 'significant improvements,' indicating general agreement on its value.

I
Interest
 High level of interest

Posts show high enthusiasm and curiosity about the method, with many expressing eagerness to learn more or apply it in their work.

E
Engagement
 Moderate level of engagement

Participants reference specific aspects such as the workflow speed and analytical precision, demonstrating active engagement and deeper understanding of the research.

I
Impact
 High level of impact

Several posts highlight the potential impact on the biopharmaceutical industry, predicting it could streamline quality control and biosimilar development significantly.

Social Mentions

YouTube

2 Videos

Twitter

1 Posts

Metrics

Video Views

170

Total Likes

1

Extended Reach

6,138

Social Features

3

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Efficient 2DLC-MS Method for Analyzing Monoclonal Antibodies in Cell Culture

Efficient 2DLC-MS Method for Analyzing Monoclonal Antibodies in Cell Culture

This study introduces a rapid 2D liquid chromatography and mass spectrometry method combining Protein A and cation exchange chromatography, significantly reducing analysis time while providing detailed charge variant profiles for mAbs and biosimilars.

Share CHO Cell research papers JP

December 7, 2025

165 views

Efficient 2DLC-MS Method for Rapid Monoclonal Antibody Analysis

Efficient 2DLC-MS Method for Rapid Monoclonal Antibody Analysis

This video discusses a new 2D liquid chromatography and mass spectrometry method that combines Protein A chromatography for titer measurement with charge variant analysis, significantly reducing analysis time and improving efficiency in biopharmaceutical studies.

Share CHO Cell research papers JP

December 10, 2025

5 views

  • BioMassSpec
    @realBioMassSpec (Twitter)

    Titer and charge-based heterogeneity multiattribute monitoring of mAbs in cell culture harvest using 2D ProA CEX MS #Talanta https://t.co/piZiwIw06j
    view full post

    May 10, 2024

Abstract Synopsis

  • The study introduces a new 2D liquid chromatography and mass spectrometry (2DLC-MS) method that combines Protein A chromatography for titer measurement with cation exchange chromatography for charge variant analysis, all in a single workflow, making the process quicker and more efficient than traditional methods.
  • The new technique uses volatile salts compatible with MS, reducing analysis time from 60–90 minutes to about 15 minutes per sample, while providing detailed charge variant profiles similar to conventional methods.
  • This method successfully quantified charge variants across a broad range of mAb concentrations and was effective in analyzing both the original mAb and its biosimilars, demonstrating its potential for rapid biosimilarity assessments in biopharmaceutical development.