Molecular biomarkers identification and applications in CHO bioprocessing.

Abstract in brief

• Biomarkers play a critical role in predicting disease susceptibility and treatment responses, and they are increasingly valuable in the biomanufacturing of therapeutic proteins by enabling data-driven approaches during cell line and process development.
• Despite the reliance on large screening methods for developing productive cell lines, there is a pressing need to transition to more rational, data-informed strategies as production limits are reached.
• The review discusses various biological molecules as biomarkers, their detection techniques, and challenges faced, while also exploring their applications and limitations in cell line and bioprocess development.