PubHawk
December 2024 PMID 39117766

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP.

Andrew Leduc, Luke Khoury, Joshua Cantlon, Saad Khan, Nikolai Slavov

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Mentions

37
Peak: 7 in Nov 2023
across 5 sources since Nov 2023

Extended reach

92,285
potential audience

Total likes

133

Video views

6,512

Mentions per month

Nov 2023 – Sep 2025

Where it's discussed

  • X / Twitter18 posts
  • YouTube8 videos
  • Bluesky5 posts
  • Facebook3 posts
  • Blogs3 articles

AI synopsis

What people are saying

The discussions reflect a high level of enthusiasm about the nPOP method, with posts describing it as a 'game changer' and emphasizing its ability to process thousands of cells swiftly. The tone and choice of words like 'massively parallel' and 'preprint' demonstrate excitement and recognition of its potential impact on single-cell proteomics research.

AgreementModerate agreement

Most discussions express support or positive anticipation for the new method, highlighting its potential benefits.

InterestHigh interest

Social discussions show high curiosity, with many posts referencing the method's technical details and implications.

EngagementModerate engagement

Several posts actively analyze the methodology, mention upcoming data, or share links to presentations, indicating moderate deep engagement.

ImpactHigh impact

The consensus suggests that the community sees this development as a significant advancement with broad applications.

Abstract in brief

  • Single-cell proteomics using mass spectrometry enables precise protein quantification, and a new method called nano-proteomic sample preparation (nPOP) allows for the simultaneous preparation of thousands of single cells in tiny droplets on glass slides.
  • The nPOP protocol is flexible for different multiplexed mass spectrometry techniques, showcasing the ability to accurately quantify 3,000-3,700 proteins per human cell using plexDIA, and analyze over 1,800 single cells daily with isobaric mass tags.
  • Implemented with the CellenONE robot and common consumables, nPOP can process >3,000 single cells in 1-2 days, and the QuantQC R package aids in quality control and
Read the full abstract

Top posts

Most liked on X and Bluesky
Slavov Laboratory
@slavovlab.bsky.social · Bluesky

The talk by @andrewleduc.bsky.social at #SCP2025 is on YouTube:

Jun 11, 2025 · 9 likes · 6 shares View post →
Pastel BioScience
@PastelBio · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/FrVTNiCKGb --- #proteomics #prot-paper https://t.co/PXJE5eDnby

Aug 10, 2024 · 6 likes · 5,376 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

@LabWelch Andrew @_AndrewLeduc talked about measuring protein synthesis and degradation rates in single cells at @SCP_meeting: https://t.co/WkNuX8R3ZH We are working on writing up these results soon, and will be happy to share the data with you.

Jul 18, 2025 · 4 likes · 2 shares · 20,844 followers View post →
Pastel BioScience
@PastelBio · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/dRljOR5m3g --- #proteomics #prot-preprint https://t.co/GBhisslTel

Nov 29, 2023 · 4 likes · 1 share · 5,376 followers View post →
Single-Cell Proteomics Conference
@SCP_meeting · X

The talk by Dr. Andrew Leduc (@_AndrewLeduc) at #SCP2025 is on YouTube: Quantification of gene expression control in a mammalian tissue at single cell resolution https://t.co/0QmvOkO2Qi

Jun 11, 2025 · 3 likes · 2 shares · 2,259 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP. https://t.co/u4cMhLv8ga

Dec 16, 2023 · 3 likes · 724 followers View post →

All mentions

Andrew Leduc
@_AndrewLeduc · X

RT @SCP_meeting: The presentation by Dr. Andrew Leduc at #SCP2025: Quantification of gene expression control in a mammalian tissue at sing…

Sep 18, 2025 · 2 shares · 744 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

RT @SCP_meeting: The presentation by Dr. Andrew Leduc at #SCP2025: Quantification of gene expression control in a mammalian tissue at sing…

Sep 18, 2025 · 2 shares · 20,844 followers View post →
Single-Cell Proteomics Conference
@SCP_meeting · X

The presentation by Dr. Andrew Leduc at #SCP2025: Quantification of gene expression control in a mammalian tissue at single cell resolution https://t.co/91Whhcuqng

Sep 18, 2025 · 2 likes · 2 shares · 2,259 followers View post →
Juan Alvarez-@juanalvarezdom.bsky.social
@JuanAlvarezDom · X

RT @slavov_n: @LabWelch Andrew @_AndrewLeduc talked about measuring protein synthesis and degradation rates in single cells at @SCP_meeting…

Jul 23, 2025 · 2 shares · 773 followers View post →
Andrew Leduc
@_AndrewLeduc · X

RT @slavov_n: @LabWelch Andrew @_AndrewLeduc talked about measuring protein synthesis and degradation rates in single cells at @SCP_meeting…

Jul 21, 2025 · 2 shares · 744 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

@LabWelch Andrew @_AndrewLeduc talked about measuring protein synthesis and degradation rates in single cells at @SCP_meeting: https://t.co/WkNuX8R3ZH We are working on writing up these results soon, and will be happy to share the data with you.

Jul 18, 2025 · 4 likes · 2 shares · 20,844 followers View post →
Andrew Leduc
@_AndrewLeduc · X

RT @SCP_meeting: The talk by Dr. Andrew Leduc (@_AndrewLeduc) at #SCP2025 is on YouTube: Quantification of gene expression control in a…

Jun 23, 2025 · 2 shares · 744 followers View post →
Slavov Laboratory
@slavovlab.bsky.social · Bluesky

The talk by @andrewleduc.bsky.social at #SCP2025 is on YouTube:

Jun 11, 2025 · 9 likes · 6 shares View post →
Prof. Nikolai Slavov
@slavov_n · X

RT @SCP_meeting: The talk by Dr. Andrew Leduc (@_AndrewLeduc) at #SCP2025 is on YouTube: Quantification of gene expression control in a…

Jun 11, 2025 · 2 shares · 20,844 followers View post →
Single-Cell Proteomics Conference
@SCP_meeting · X

The talk by Dr. Andrew Leduc (@_AndrewLeduc) at #SCP2025 is on YouTube: Quantification of gene expression control in a mammalian tissue at single cell resolution https://t.co/0QmvOkO2Qi

Jun 11, 2025 · 3 likes · 2 shares · 2,259 followers View post →
Ronnie Cutler
@cutleraging · X

@Nick_Schaum Using the TMT based multiplexing https://t.co/y5Qexeu1fQ. Churchill has shown that the correlation between protein and RNA decreases during aging. 10X multiome https://t.co/3BYCSQwI8V to decipher tx noise source Or 10X scNRA w/ long reads for stochastic splicing dysreg

Nov 1, 2024 · 463 followers View post →
PastelBio
@pastelbio.bsky.social · Bluesky

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP www.nature.com/artic... --- #proteomics #prot-paper

Aug 10, 2024 View post →
Pastel BioScience
@PastelBio · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/FrVTNiCKGb --- #proteomics #prot-paper https://t.co/PXJE5eDnby

Aug 10, 2024 · 6 likes · 5,376 followers View post →
Kermit Murray
@kermitmurray · X

(BioRxiv All) Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/hlVkSFLjKY (RSS) #BioRxiv #MassSpecRSS https://t.co/UIVbKW7Rzm

Dec 21, 2023 · 1,968 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP. https://t.co/u4cMhLv8ga

Dec 16, 2023 · 3 likes · 724 followers View post →
J of Mass Spec & Advances in the Clinical Lab
@JMSACL · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/hjHMUQpBQc

Dec 11, 2023 · 1 like · 1,009 followers View post →
Mass-spec@RIKEN BDR KOBE
@bdr_massspec · X

RT @PastelBio: Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/dRljOR5m3g --- #proteo…

Nov 29, 2023 · 1 share · 68 followers View post →
PastelBio
@pastelbio.bsky.social · Bluesky

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP www.biorxiv.org/cont... --- #proteomics #prot-preprint

Nov 29, 2023 · 2 likes · 1 share View post →
Pastel BioScience
@PastelBio · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/dRljOR5m3g --- #proteomics #prot-preprint https://t.co/GBhisslTel

Nov 29, 2023 · 4 likes · 1 share · 5,376 followers View post →
MD BABU MIA, PHD
@BabuuDx · X

Great preprint of "Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP" from @slavovLab Look at the figure 5! https://t.co/JYGRzKDR7S

Nov 28, 2023 · 3 likes · 476 followers View post →
bioRxiv Bioengineering
@bioRxiv_bioE · X

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/r0NF8dCQgW #biorxiv_bioE

Nov 28, 2023 · 206 followers View post →
bioRxiv Bioengineering
@biorxiv-bioeng.bsky.social · Bluesky

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://www.biorxiv.org/content/10.1101/2023.11.27.568927v1

Nov 28, 2023 View post →
bioRxivpreprint
@biorxivpreprint.bsky.social · Bluesky

Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://www.biorxiv.org/content/10.1101/2023.11.27.568927v1

Nov 28, 2023 View post →