Chem Rev January 2025 PMID 39700225

Cell-Free Gene Expression: Methods and Applications.

Andrew C Hunt, Blake J Rasor, Kosuke Seki, Holly M Ekas, Katherine F Warfel, Ashty S Karim, Michael C Jewett

Mentions

25
Peak: 23 in Dec 2024
across 3 sources since Dec 2024

Extended reach

43,846
potential audience

Total likes

91

Video views

147

Mentions per month

Dec 2024 – May 2025

Where it's discussed

  • X / Twitter22 posts
  • YouTube2 videos
  • Bluesky1 posts

AI synopsis

What people are saying

Overall, the discussions convey strong enthusiasm for the article, with examples like calling it a 'guide I wish I had' and noting its potential to revolutionize applications. Words such as 'future' and 'opportunities' demonstrate excitement and recognition of the article’s significance, leading to high interest and engagement among participants.

AgreementModerate agreement

Most discussions express enthusiasm and support for the relevance and potential of cell-free gene expression, highlighting its importance for future applications and scientific progress.

InterestHigh interest

Posts frequently mention specific benefits and opportunities in manufacturing, diagnostics, and synthetic biology, indicating high curiosity and engagement with the topic.

EngagementHigh engagement

Several comments reference personal experiences, acknowledgments of the review’s usefulness, and calls to explore the subject further, showing deep involvement.

ImpactModerate impact

The discussions suggest that the publication could influence future research directions, with mentions of innovations and practical applications in various fields.

Abstract in brief

  • Cell-free gene expression (CFE) systems allow scientists to create biological molecules and processes outside of living cells by using purified enzymes or cell lysates, enabling more precise and complex biomolecular transformations.
  • These systems bypass many limitations of traditional cell-based methods, such as molecular transport constraints and cellular survival mechanisms, and can be easily distributed and stored through freeze-drying, making them practical for various applications.
  • Owing to their nonliving nature, CFE systems also offer built-in safety features for biocontainment and are revolutionizing efforts in synthetic biology, including building genetic networks and manufacturing biobased products.

Top posts

Most liked on X and Bluesky
Michael C. Jewett
@MichaelCJewett · X

Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diagnostics, and education? Check out our open access comprehensive review, with @akarimlab @Andrew_C_Hunt Blake Rasor @ktwarfel @NUSynBio https://t.co/OAbU3iWZVT

Dec 19, 2024 · 48 likes · 12 shares · 8,039 followers View post →
Andrew Hunt
@Andrew_C_Hunt · X

Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I started my PhD. Grateful to my coauthors and reviewers for helping tackle this giant project. We hope you find it helpful! https://t.co/OdzefKw2LO https://t.co/6A0pJpZnl2

Dec 19, 2024 · 22 likes · 4 shares · 346 followers View post →
Katsumi Hagino (萩野 勝己)
@Hagi_k_ron · X

教科書 Cell-Free Gene Expression: Methods and Applications | Chemical Reviews https://t.co/92CFsRBC5N

Dec 19, 2024 · 6 likes · 368 followers View post →
Olivier Borkowski
@oborkowski.bsky.social · Bluesky

Cell-Free Gene Expression: Methods and Applications doi.org/10.1021/acs....

Dec 19, 2024 · 5 likes · 3 shares View post →
Xuan (Stephenson) Lai
@XuanLaiTW · X

#PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we need in the future, especially for expressing those toxic effectors. https://t.co/2YAWpdihBd

Dec 21, 2024 · 2 likes · 2 shares · 129 followers View post →
ACS Macro Journals
@MacroJrnls_ACS · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 20, 2024 · 12 shares · 9,248 followers View post →

All mentions

Grace Pruksarojanakul
@gracedpst · X

RT @XuanLaiTW: #PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we ne…

Dec 23, 2024 · 2 shares · 244 followers View post →
Erh-Min Lai
@erhminlai · X

RT @XuanLaiTW: #PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we ne…

Dec 22, 2024 · 2 shares · 689 followers View post →
Xuan (Stephenson) Lai
@XuanLaiTW · X

#PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we need in the future, especially for expressing those toxic effectors. https://t.co/2YAWpdihBd

Dec 21, 2024 · 2 likes · 2 shares · 129 followers View post →
Ari Satanowski
@AriSatanowski · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 21, 2024 · 12 shares · 214 followers View post →
Nitin Bohra
@nitinbohra3 · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 20, 2024 · 12 shares · 182 followers View post →
ACS Macro Journals
@MacroJrnls_ACS · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 20, 2024 · 12 shares · 9,248 followers View post →
Athale Lab: CyCelS
@AthaleLab · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 20, 2024 · 12 shares · 1,509 followers View post →
Javin Oza
@ozalabCP · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 20, 2024 · 12 shares · 828 followers View post →
Yotam Feldman
@yotamfe · X

RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…

Dec 20, 2024 · 4 shares · 403 followers View post →
Filippo Caschera
@Stjle · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 20, 2024 · 12 shares · 476 followers View post →
Katsumi Hagino (萩野 勝己)
@Hagi_k_ron · X

教科書 Cell-Free Gene Expression: Methods and Applications | Chemical Reviews https://t.co/92CFsRBC5N

Dec 19, 2024 · 6 likes · 368 followers View post →
Kosuke Seki
@kosuke_seki · X

RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…

Dec 19, 2024 · 4 shares · 85 followers View post →
WeiTing Lin(GR15
@weitinglin66 · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 19, 2024 · 12 shares · 1,287 followers View post →
Michael C. Jewett
@MichaelCJewett · X

RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…

Dec 19, 2024 · 4 shares · 8,039 followers View post →
Shingo Honda (本田 信吾)
@akg_entrance · X

RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…

Dec 19, 2024 · 4 shares · 1,541 followers View post →
Andrew Hunt
@Andrew_C_Hunt · X

Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I started my PhD. Grateful to my coauthors and reviewers for helping tackle this giant project. We hope you find it helpful! https://t.co/OdzefKw2LO https://t.co/6A0pJpZnl2

Dec 19, 2024 · 22 likes · 4 shares · 346 followers View post →
Khalid K. Alam ‎خالد كمال عالم
@BiochemPhD · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 19, 2024 · 12 shares · 1,555 followers View post →
Megan McSweeney
@MeganAMcSweeney · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 19, 2024 · 12 shares · 32 followers View post →
Olivier Borkowski
@oborkowski.bsky.social · Bluesky

Cell-Free Gene Expression: Methods and Applications doi.org/10.1021/acs....

Dec 19, 2024 · 5 likes · 3 shares View post →
Olivier Borkowski
@O_Borkowski · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 19, 2024 · 12 shares · 7,013 followers View post →
Beau Dronsella
@BDronsella · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 19, 2024 · 12 shares · 313 followers View post →
Gentle Mutt
@GentleMutt · X

RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…

Dec 19, 2024 · 12 shares · 790 followers View post →
Michael C. Jewett
@MichaelCJewett · X

Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diagnostics, and education? Check out our open access comprehensive review, with @akarimlab @Andrew_C_Hunt Blake Rasor @ktwarfel @NUSynBio https://t.co/OAbU3iWZVT

Dec 19, 2024 · 48 likes · 12 shares · 8,039 followers View post →