Cell-Free Gene Expression: Methods and Applications.

Andrew C Hunt, Blake J Rasor, Kosuke Seki, Holly M Ekas, Katherine F Warfel, Ashty S Karim, Michael C Jewett

January 2025 Chem Rev

Synopsis of Social media discussions

Overall, the discussions convey strong enthusiasm for the article, with examples like calling it a 'guide I wish I had' and noting its potential to revolutionize applications. Words such as 'future' and 'opportunities' demonstrate excitement and recognition of the article’s significance, leading to high interest and engagement among participants.

A
Agreement
 Moderate agreement

Most discussions express enthusiasm and support for the relevance and potential of cell-free gene expression, highlighting its importance for future applications and scientific progress.

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Interest
 High level of interest

Posts frequently mention specific benefits and opportunities in manufacturing, diagnostics, and synthetic biology, indicating high curiosity and engagement with the topic.

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Engagement
 High engagement

Several comments reference personal experiences, acknowledgments of the review’s usefulness, and calls to explore the subject further, showing deep involvement.

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Impact
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The discussions suggest that the publication could influence future research directions, with mentions of innovations and practical applications in various fields.

Social Mentions

YouTube

2 Videos

Bluesky

1 Posts

Twitter

22 Posts

Metrics

Video Views

147

Total Likes

91

Extended Reach

43,846

Social Features

25

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

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May 30, 2025

110 views

Advances in Cost-Effective Cell-Free Gene Expression Systems

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Cell-free gene expression systems enable biomolecular production outside living cells, overcoming traditional limitations and offering safety and ease of distribution for synthetic biology applications.

theicechol

May 30, 2025

38 views

  • Grace Pruksarojanakul
    @gracedpst (Twitter)

    RT @XuanLaiTW: #PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we ne…
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    December 23, 2024

    2

  • Erh-Min Lai
    @erhminlai (Twitter)

    RT @XuanLaiTW: #PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we ne…
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    December 22, 2024

    2

  • Xuan (Stephenson) Lai
    @XuanLaiTW (Twitter)

    #PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we need in the future, especially for expressing those toxic effectors. https://t.co/2YAWpdihBd
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    December 21, 2024

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  • Ari Satanowski
    @AriSatanowski (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 21, 2024

    12

  • Nitin Bohra
    @nitinbohra3 (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 20, 2024

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  • ACS Macro Journals
    @MacroJrnls_ACS (Twitter)

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    December 20, 2024

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  • Athale Lab: CyCelS
    @AthaleLab (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 20, 2024

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  • Javin Oza
    @ozalabCP (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 20, 2024

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  • Yotam Feldman
    @yotamfe (Twitter)

    RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…
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    December 20, 2024

    4

  • Filippo Caschera
    @Stjle (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 20, 2024

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  • Katsumi Hagino (萩野 勝己)
    @Hagi_k_ron (Twitter)

    教科書 Cell-Free Gene Expression: Methods and Applications | Chemical Reviews https://t.co/92CFsRBC5N
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    December 19, 2024

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  • Kosuke Seki
    @kosuke_seki (Twitter)

    RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…
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    December 19, 2024

    4

  • WeiTing Lin(GR15
    @weitinglin66 (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 19, 2024

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  • Michael C. Jewett
    @MichaelCJewett (Twitter)

    RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…
    view full post

    December 19, 2024

    4

  • Shingo Honda (本田 信吾)
    @akg_entrance (Twitter)

    RT @Andrew_C_Hunt: Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I starte…
    view full post

    December 19, 2024

    4

  • Andrew Hunt
    @Andrew_C_Hunt (Twitter)

    Curious about cell-free systems? Check out our new review in @ACSChemRev! This is the guide I wish I’d had when I started my PhD. Grateful to my coauthors and reviewers for helping tackle this giant project. We hope you find it helpful! https://t.co/OdzefKw2LO https://t.co/6A0pJpZnl2
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    December 19, 2024

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  • Khalid K. Alam ‎خالد كمال عالم
    @BiochemPhD (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 19, 2024

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  • Megan McSweeney
    @MeganAMcSweeney (Twitter)

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    December 19, 2024

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  • Olivier Borkowski
    @oborkowski.bsky.social (Bluesky)

    Cell-Free Gene Expression: Methods and Applications doi.org/10.1021/acs....
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    December 19, 2024

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    @O_Borkowski (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 19, 2024

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  • Beau Dronsella
    @BDronsella (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 19, 2024

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  • Gentle Mutt
    @GentleMutt (Twitter)

    RT @MichaelCJewett: Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diag…
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    December 19, 2024

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  • Michael C. Jewett
    @MichaelCJewett (Twitter)

    Looking for some holiday reading? Wondering how #cellfree systems are creating new opportunities in manufacturing, diagnostics, and education? Check out our open access comprehensive review, with @akarimlab @Andrew_C_Hunt Blake Rasor @ktwarfel @NUSynBio https://t.co/OAbU3iWZVT
    view full post

    December 19, 2024

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Abstract Synopsis

  • Cell-free gene expression (CFE) systems allow scientists to create biological molecules and processes outside of living cells by using purified enzymes or cell lysates, enabling more precise and complex biomolecular transformations.
  • These systems bypass many limitations of traditional cell-based methods, such as molecular transport constraints and cellular survival mechanisms, and can be easily distributed and stored through freeze-drying, making them practical for various applications.
  • Owing to their nonliving nature, CFE systems also offer built-in safety features for biocontainment and are revolutionizing efforts in synthetic biology, including building genetic networks and manufacturing biobased products.