Publications by authors named "Birgit Wiltschi"

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A Robust Bioprocess for the Global Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts Produces Labeled Proteins at the Gram Scale.
Patrik Fladischer, Niklaus Anderhuber, Octavian Murgu, Altijana Hromić-Jahjefendić, Tea Pavkov-Keller, Birgit Wiltschi

Chembiochem· December 2025

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Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:
Žana Marin, Claudia Lacombe, Simindokht Rostami, Arshia Arasteh Kani, Andrea Borgonovo, Birgit Wiltschi

Chem Rev· May 2025

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Protocol for protein modification using oxalyl thioester-mediated chemoselective ligation.
Francesco Terzani, Chen Wang, Simindokht Rostami, Rémi Desmet, Benoît Snella, Birgit Wiltschi

STAR Protoc· December 2024

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Site-Specific Immobilization Boosts the Performance of a Galectin-1 Biosensor.
Dajana Kolanovic, Rajeev Pasupuleti, Jakob Wallner, Georg Mlynek, Birgit Wiltschi

Bioconjug Chem· December 2024

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Aligning fermentation conditions with non-canonical amino acid addition strategy is essential for Nε-((2-azidoethoxy)carbonyl)-L-lysine uptake and incorporation into the target protein.
Hana Hanaee-Ahvaz, Marina Alexandra Baumann, Christopher Tauer, Bernd Albrecht, Birgit Wiltschi

Sci Rep· October 2024

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Lectin-anticancer peptide fusion demonstrates a significant cancer-cell-selective cytotoxic effect and inspires the production of "clickable" anticancer peptide in Escherichia coli.
Rajeev Pasupuleti, Sabrina Riedl, Laia Saltor Núñez, Marianna Karava, Vajinder Kumar, Birgit Wiltschi

Protein Sci· December 2023

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Antibody fragments functionalized with non-canonical amino acids preserving structure and functionality - A door opener for new biological and therapeutic applications.
Hana Hanaee-Ahvaz, Monika Cserjan-Puschmann, Florian Mayer, Christopher Tauer, Bernd Albrecht, Birgit Wiltschi

Heliyon· December 2023

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Genetic code expansion in E. coli enables production of a functional 'ready-to-click' T cell receptor-specific scFv.
Rajeev Pasupuleti, Francesca Rosato, Dajana Kolanovic, Olga N Makshakova, Winfried Römer, Birgit Wiltschi

N Biotechnol· September 2023

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Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy.
Natalia Danielewicz, Francesca Rosato, Jana Tomisch, Jonas Gräber, Birgit Wiltschi

ACS Omega· May 2023

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Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coli.
Julia Jodlbauer, Lukas Rieder, Anton Glieder, Birgit Wiltschi

Methods Mol Biol· January 2023

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A bispecific, crosslinking lectibody activates cytotoxic T cells and induces cancer cell death.
Francesca Rosato, Rajeev Pasupuleti, Jana Tomisch, Ana Valeria Meléndez, Dajana Kolanovic, Birgit Wiltschi

J Transl Med· December 2022

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One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins.
Felix Tobola, Birgit Wiltschi

Biotechnol Adv· November 2022

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Fast Protein Modification in the Nanomolar Concentration Range Using an Oxalyl Amide as Latent Thioester.
Benoît Snella, Benjamin Grain, Jérôme Vicogne, Frédéric Capet, Birgit Wiltschi

Angew Chem Int Ed Engl· July 2022

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Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production.
Katharina N Schwaiger, Alena Voit, Birgit Wiltschi, Bernd Nidetzky

Microb Cell Fact· April 2022

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Engineering the Ligand Specificity of the Human Galectin-1 by Incorporation of Tryptophan Analogues.
Felix Tobola, Martin Lepšík, Syeda Rehana Zia, Hakon Leffler, Ulf J Nilsson, Birgit Wiltschi

Chembiochem· March 2022

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Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters.
Karolina Haernvall, Patrik Fladischer, Heidemarie Schoeffmann, Sabine Zitzenbacher, Tea Pavkov-Keller, Birgit Wiltschi

Front Bioeng Biotechnol· January 2022

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Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose.
Katharina N Schwaiger, Alena Voit, Hana Dobiašová, Christiane Luley, Birgit Wiltschi

Biotechnol J· November 2020

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Decoupling Protein Production from Cell Growth Enhances the Site-Specific Incorporation of Noncanonical Amino Acids in .
Meritxell Galindo Casas, Patrick Stargardt, Juergen Mairhofer, Birgit Wiltschi

ACS Synth Biol· November 2020

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Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications.
Birgit Wiltschi, Tomislav Cernava, Alexander Dennig, Meritxell Galindo Casas, Martina Geier

Biotechnol Adv· May 2020

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'Clickable lectins': bioorthogonal reactive handles facilitate the directed conjugation of lectins in a modular fashion.
Felix Tobola, Elise Sylvander, Claudia Gafko, Birgit Wiltschi

Interface Focus· April 2019

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A Semi-Rationally Engineered Bacterial Pyrrolysyl-tRNA Synthetase Genetically Encodes Phenyl Azide Chemistry.
Patrik Fladischer, Alexandra Weingartner, Johannes Blamauer, Barbara Darnhofer, Ruth Birner-Gruenberger, Birgit Wiltschi

Biotechnol J· March 2019

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Substituting the catalytic proline of 4-oxalocrotonate tautomerase with non-canonical analogues reveals a finely tuned catalytic system.
Michael S Lukesch, Tea Pavkov-Keller, Karl Gruber, Klaus Zangger, Birgit Wiltschi

Sci Rep· February 2019

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Effect of Noncanonical Amino Acids on Protein-Carbohydrate Interactions: Structure, Dynamics, and Carbohydrate Affinity of a Lectin Engineered with Fluorinated Tryptophan Analogs.
Felix Tobola, Mickael Lelimousin, Annabelle Varrot, Emilie Gillon, Barbara Darnhofer, Birgit Wiltschi

ACS Chem Biol· August 2018

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Molecular cloning, expression, and characterization of acyltransferase from Pseudomonas protegens.
Nina G Schmidt, Anna Żądło-Dobrowolska, Valerie Ruppert, Christian Höflehner, Birgit Wiltschi

Appl Microbiol Biotechnol· July 2018

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Biocatalytic Friedel-Crafts Acylation and Fries Reaction.
Nina G Schmidt, Tea Pavkov-Keller, Nina Richter, Birgit Wiltschi, Karl Gruber

Angew Chem Int Ed Engl· June 2017

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