Publications by authors named "John Heap"

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Modular Combinatorial DNA Assembly of Group B Streptococcus Capsular Polysaccharide Biosynthesis Pathways to Expediate the Production of Novel Glycoconjugate Vaccines.
Mark A Harrison, Elizabeth Atkins, Alexandra Faulds-Pain, John T Heap, Brendan W Wren

Vaccines (Basel)· March 2025

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A primer to directed evolution: current methodologies and future directions.
Lara Sellés Vidal, Mark Isalan, John T Heap, Rodrigo Ledesma-Amaro

RSC Chem Biol· April 2023

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A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli.
Ian J Passmore, Alexandra Faulds-Pain, Sherif Abouelhadid, Mark A Harrison, Catherine L Hall, John T Heap

Glycobiology· March 2023

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Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.
João P C Moreira, John T Heap, Joana I Alves, Lucília Domingues

Biotechnol Biofuels Bioprod· February 2023

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Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria.
George M Taylor, Andrew Hitchcock, John T Heap

Nucleic Acids Res· December 2021

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Versatile selective evolutionary pressure using synthetic defect in universal metabolism.
Lara Sellés Vidal, James W Murray, John T Heap

Nat Commun· November 2021

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Design and Implementation of Multi-protein Expression Constructs and Combinatorial Libraries using Start-Stop Assembly.
George M Taylor, John T Heap

Methods Mol Biol· March 2021

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Type IV Pili-Independent Photocurrent Production by the Cyanobacterium sp. PCC 6803.
Miyuki A Thirumurthy, Andrew Hitchcock, Angelo Cereda, Jiawei Liu, Marko S Chavez, John T Heap

Front Microbiol· June 2020

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VAPPER: High-throughput variant antigen profiling in African trypanosomes of livestock.
Sara Silva Pereira, John Heap, Andrew R Jones, Andrew P Jackson

Gigascience· September 2019

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Transcriptional Terminators Allow Leak-Free Chromosomal Integration of Genetic Constructs in Cyanobacteria.
Ciarán L Kelly, George M Taylor, Aistė Šatkutė, Linda Dekker, John T Heap

Microorganisms· August 2019

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Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering.
George M Taylor, Paweł M Mordaka, John T Heap

Nucleic Acids Res· February 2019

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The Butanol Producing Microbe Clostridium beijerinckii NCIMB 14988 Manipulated Using Forward and Reverse Genetic Tools.
Gareth T Little, Benjamin J Willson, John T Heap, Klaus Winzer, Nigel P Minton

Biotechnol J· November 2018

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Synthetic negative feedback circuits using engineered small RNAs.
Ciarán L Kelly, Andreas W K Harris, Harrison Steel, Edward J Hancock, John T Heap

Nucleic Acids Res· October 2018

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A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria.
Ciarán L Kelly, George M Taylor, Andrew Hitchcock, Antonio Torres-Méndez, John T Heap

ACS Synth Biol· April 2018

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Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application.
Lara Sellés Vidal, Ciarán L Kelly, Paweł M Mordaka, John T Heap

Biochim Biophys Acta Proteins Proteom· February 2018

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Stringency of Synthetic Promoter Sequences in Clostridium Revealed and Circumvented by Tuning Promoter Library Mutation Rates.
Paweł M Mordaka, John T Heap

ACS Synth Biol· February 2018

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Inactivation of the dnaK gene in Clostridium difficile 630 Δerm yields a temperature-sensitive phenotype and increases biofilm-forming ability.
Shailesh Jain, Deborah Smyth, Barry M G O'Hagan, John T Heap, Geoff McMullan

Sci Rep· December 2017

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Susceptibility to Closthioamide among Clinical and Reference Strains of Neisseria gonorrhoeae.
Victoria F Miari, Priya Solanki, Yonek Hleba, Richard A Stabler, John T Heap

Antimicrob Agents Chemother· October 2017

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A genetic assay for gene essentiality in Clostridium.
David J F Walker, John T Heap, Klaus Winzer, Nigel P Minton

Anaerobe· December 2016

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Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the rhaBAD Promoter.
Ciarán L Kelly, Zilei Liu, Akihide Yoshihara, Sarah F Jenkinson, Mark R Wormald, John T Heap

ACS Synth Biol· October 2016

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6-Deoxyhexoses from l-Rhamnose in the Search for Inducers of the Rhamnose Operon: Synergy of Chemistry and Biotechnology.
Zilei Liu, Akihide Yoshihara, Ciarán Kelly, John T Heap, Mikkel H S Marqvorsen

Chemistry· August 2016

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Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.
Muhammad Ehsaan, Wouter Kuit, Ying Zhang, Stephen T Cartman, John T Heap

Biotechnol Biofuels· January 2016

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Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo.
John T Heap, Jan Theys, Muhammad Ehsaan, Aleksandra M Kubiak, Ludwig Dubois

Oncotarget· April 2014

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Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.
Katalin Kovács, Benjamin J Willson, Katrin Schwarz, John T Heap, Adam Jackson

Biotechnol Biofuels· August 2013

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Two-component signal transduction system CBO0787/CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502.
Zhen Zhang, Hannu Korkeala, Elias Dahlsten, Elina Sahala, John T Heap

PLoS Pathog· March 2013

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