Publications by authors named "Tetsuo Torisu"

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Variation of VP2 stoichiometry and deamidation of VP1 during production and their impacts on the transduction efficiency of AAV vectors.
Takahiro Maruno, Mitsuko Fukuhara, Yasuo Tsunaka, Aoba Matsushita, Kiichi Hirohata, Tetsuo Torisu

Mol Ther Methods Clin Dev· December 2025

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Effect of temperature on the aggregation of an Fc-fusion protein under agitation.
Zekun Wang, Arni Gambe-Gilbuena, Satoru Unzai, Susumu Uchiyama, Tetsuo Torisu

Eur J Pharm Sci· December 2025

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Heat-Induced Changes in the Chemical Structure, Hydrophobicity, and Size Distribution of Free/Bound Lipid Transfer Protein 1 and Their Effects on Beer Foam.
Junya Mizuguchi, Kihiro Asaka, Moe Konishi, Takako Inui, Susumu Uchiyama, Tetsuo Torisu

J Agric Food Chem· September 2025

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Use of cesium chloride density gradient ultracentrifugation for the purification and characterization of recombinant adeno-associated virus.
Kiichi Hirohata, Shinichiro Kino, Takuya Yamane, Karin Bandoh, Takeshi Bamba, Tetsuo Torisu

Eur Biophys J· August 2025

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Unfolding of viral protein 1 N-termini facilitates genome ejection from recombinant adeno-associated virus serotype 8.
Yuki Yamaguchi, Saki Shimojo, Tomohiko Ikeda, Mitsuko Fukuhara, Yasuo Tsunaka, Tetsuo Torisu

Mol Ther Methods Clin Dev· June 2025

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H
Hierarchical clustering of therapeutic proteins based on agitation-induced aggregation propensity and its relation to physicochemical parameters.
Rio Okada, Kosei Shibata, Risa Shibuya, Tetsuo Torisu, Susumu Uchiyama

Eur J Pharm Sci· May 2025

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Direct Identification and Quantification of Recombinant Adeno-Associated Virus in Crude Cell Lysate and Conditioned Medium by Mass Photometry.
Yuki Yamaguchi, Saki Shimojo, Risa Shibuya, Karin Bandoh, Aoba Matsushita, Tetsuo Torisu

Anal Chem· May 2025

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Survey and Establishment of Points to Consider for Application of Analytical Techniques to Evaluate Protein Aggregates and Insoluble Particles in Biopharmaceuticals: Experiences in Japan Biopharmaceutical Consortium.
Hiroko Shibata, Satoshi Saitoh, Masato Kiyoshi, Yu Hayashi, Kazue Inaba, Tetsuo Torisu

AAPS J· April 2025

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Protein aggregation in the frozen state induced by dropping stress.
Tetsuo Torisu, Ayuko Maeda, Shuhei Ito, Susumu Uchiyama

Eur J Pharm Sci· February 2025

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I
Impact of Weak Vibration Generated by a Refrigerator on Protein Aggregation.
Shinji Kizuki, Zekun Wang, Satoru Yamauchi, Tetsuo Torisu, Susumu Uchiyama

AAPS J· January 2025

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Physicochemical and biological impacts of light stress on adeno-associated virus serotype 6.
Rie Takino, Yuki Yamaguchi, Takahiro Maruno, Ekaputra Ramadhani, Misaki Furukawa, Tetsuo Torisu

Mol Ther Methods Clin Dev· December 2024

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Multimass Analysis of Adeno-Associated Virus Vectors by Orbitrap-Based Charge Detection Mass Spectrometry.
Ryoji Nakatsuka, Yuki Yamaguchi, Kiichi Hirohata, Saki Shimojo, Makoto Murakami, Tetsuo Torisu

Anal Chem· October 2024

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Quantification of full and empty particles of adeno-associated virus vectors via a novel dual fluorescence-linked immunosorbent assay.
Sereirath Soth, Mikako Takakura, Masahiro Suekawa, Takayuki Onishi, Kiichi Hirohata, Tetsuo Torisu

Mol Ther Methods Clin Dev· September 2024

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C
Combined 100 keV Cryo-Electron Microscopy and Image Analysis Methods to Characterize the Wider Adeno-Associated Viral Products.
Haruka Nishiumi, Kiichi Hirohata, Mitsuko Fukuhara, Aoba Matsushita, Yasuo Tsunaka, Tetsuo Torisu

J Pharm Sci· July 2024

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Prediction models for the flux decay profile and initial flux of microfiltration for therapeutic proteins.
Kota Inoue, Yumiko Masuda, Tetsuo Torisu, Koichi Nonaka, Susumu Uchiyama

Biotechnol Bioeng· June 2024

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Glycosylation of recombinant adeno-associated virus serotype 6.
Yuki Yamaguchi, Kentaro Ishii, Sachiko Koizumi, Hiroaki Sakaue, Takahiro Maruno, Tetsuo Torisu

Mol Ther Methods Clin Dev· June 2024

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T
Three-Dimensional Homodyne Light Detection (3D-HLD) for High-Throughput Submicron Particle Analysis in (Highly Concentrated) Protein Biopharmaceuticals, Viral Vectors, and LNPs.
Dominik Brandstetter, Constanze Helbig, Kentaro Osawa, Hiroyuki Minemura, Yumiko Anzai, Tetsuo Torisu

J Pharm Sci· April 2024

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Structure of Human Serum Albumin at a Foam Surface.
Kanta Enomoto, Tetsuo Torisu, Junya Mizuguchi, Ryosuke Tanoue, Susumu Uchiyama

J Agric Food Chem· April 2024

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Higher-Order Structure of Adeno-Associated Virus Serotype 8 by Hydrogen/Deuterium Exchange Mass Spectrometry.
Tomohiko Ikeda, Yuki Yamaguchi, Hiroaki Oyama, Aoba Matsushita, Yasuo Tsunaka, Tetsuo Torisu

Viruses· April 2024

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A
Applications and Limitations of Equilibrium Density Gradient Analytical Ultracentrifugation for the Quantitative Characterization of Adeno-Associated Virus Vectors.
Kiichi Hirohata, Yuki Yamaguchi, Takahiro Maruno, Risa Shibuya, Tetsuo Torisu

Anal Chem· January 2024

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Optimization of Flow Imaging Microscopy Setting Using Spherical Beads with Optical Properties Similar to Those of Biopharmaceuticals.
Takaaki Kurinomaru, Kimitoshi Takeda, Megumi Onaka, Yuki Kuruma, Keiji Takahata, Tetsuo Torisu

J Pharm Sci· December 2023

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E
Enhancement of recombinant adeno-associated virus activity by improved stoichiometry and homogeneity of capsid protein assembly.
Takayuki Onishi, Michika Nonaka, Takahiro Maruno, Yuki Yamaguchi, Mitsuko Fukuhara, Tetsuo Torisu

Mol Ther Methods Clin Dev· December 2023

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S
Size Distribution Analysis of the Adeno-Associated Virus Vector by the c(s) Analysis of Band Sedimentation Analytical Ultracentrifugation with Multiwavelength Detection.
Takahiro Maruno, Kentaro Ishii, Tetsuo Torisu, Susumu Uchiyama

J Pharm Sci· April 2023

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Relationship between aggregation of therapeutic proteins and agitation parameters: Acceleration and frequency.
Shinji Kizuki, Zekun Wang, Tetsuo Torisu, Satoru Yamauchi, Susumu Uchiyama

J Pharm Sci· February 2023

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Utility of Three Flow Imaging Microscopy Instruments for Image Analysis in Evaluating four Types of Subvisible Particle in Biopharmaceuticals.
Haruka Nishiumi, Natalie Deiringer, Nils Krause, Saki Yoneda, Tetsuo Torisu

J Pharm Sci· November 2022

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