PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, enzymatic base editing and the new prime editing technique for the precise genome editing with CRISPR. #CRISPR #Cas9 https://t.co/gB7AkC8dZF via @YouTube
A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells.
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Feb 2020All 6 dated mentions so far are from Feb 2020.
Where it's discussed
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X / Twitter4 posts
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YouTube2 videos
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Facebook2 posts
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Blogs2 articles
AI synopsis
What people are saying
The discussions are centered around technological improvements in genome editing, mentioning terms like 'precision editing', 'HDR', and 'improving CRISPR specificity', which imply a focus on understanding and applying these methods rather than fully analyzing the research findings. Users use phrases like 'describe the use of HDR' and 'strategies that can greatly improve', indicating curiosity and engagement with the technical aspects, but there is limited expression of strong opinions or high-impact implications.
The discussions mainly acknowledge the importance of precision gene editing techniques, but do not explicitly praise or criticize the specific publication.
Posts show moderate interest, focusing on general improvements in CRISPR technology rather than the specific Cas9 variant detailed in the article.
The posts demonstrate some engagement by referencing specific methods like HDR and strategies to enhance specificity, indicating an interest in technical details.
While the discussions highlight the technological advances in gene editing, they do not explicitly state the immediate impact of this new Cas9 variant, suggesting moderate perceived significance.
Abstract in brief
- The researchers developed a modified version of the Cas9 enzyme called SpCas9Gem, which is designed to be broken down during the G1 phase of the cell cycle to reduce unwanted mutations from NHEJ, a process that often causes errors in gene editing.
- They used mRNA transfection to deliver this SpCas9Gem, allowing for quick and temporary expression, which helps prevent long-term off-target effects and improves the precision of gene editing in human pluripotent stem cells.
- This new approach significantly decreases undesired mutations at the target site while still enabling efficient creation of gene knock-ins and corrected patient-specific stem cell lines, making gene editing safer and more reliable.]
Top posts
Most liked on X and BlueskyHOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can be used to greatly improve the specificity of the S.pyogenes CRISPR/Cas9 system during genome editing. #CRISPR #Cas9 https://t.co/bEStlEe85w via @YouTube
RT @GenomicsGurus: PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, en…
RT @GenomicsGurus: HOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can…
All mentions
Precision Genome Editing with Prime Editing Techniques
This presentation describes precise genome editing techniques, including Prime Editing, which allows for direct genetic insertion without double-strand breaks. This new method shows higher efficiency and fewer…
Enhancing CRISPR Specificity for Precise Genome Editing
This presentation explains four key strategies to improve CRISPR-Cas9 specificity, including dual nickases, knockout cell lines, and enhanced Cas9 mutants. These advancements expand precise genome editing applications…
RT @GenomicsGurus: PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, en…
PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, enzymatic base editing and the new prime editing technique for the precise genome editing with CRISPR. #CRISPR #Cas9 https://t.co/gB7AkC8dZF via @YouTube
RT @GenomicsGurus: HOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can…
HOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can be used to greatly improve the specificity of the S.pyogenes CRISPR/Cas9 system during genome editing. #CRISPR #Cas9 https://t.co/bEStlEe85w via @YouTube