Stem Cell Reports September 2016 PMID 27499201

A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells.

Sara E Howden, Bradley McColl, Astrid Glaser, Jim Vadolas, Steven Petrou, Melissa H Little, Andrew G Elefanty, Edouard G Stanley

Mentions

10
Peak: 6 in Feb 2020
across 4 sources since Feb 2020

Extended reach

26,093
potential audience

Total likes

365

Video views

25,344

Mentions per month

Feb 2020

All 6 dated mentions so far are from Feb 2020.

Where it's discussed

  • X / Twitter4 posts
  • YouTube2 videos
  • Facebook2 posts
  • Blogs2 articles

AI synopsis

What people are saying

The discussions are centered around technological improvements in genome editing, mentioning terms like 'precision editing', 'HDR', and 'improving CRISPR specificity', which imply a focus on understanding and applying these methods rather than fully analyzing the research findings. Users use phrases like 'describe the use of HDR' and 'strategies that can greatly improve', indicating curiosity and engagement with the technical aspects, but there is limited expression of strong opinions or high-impact implications.

AgreementNeither agree nor disagree

The discussions mainly acknowledge the importance of precision gene editing techniques, but do not explicitly praise or criticize the specific publication.

InterestModerate interest

Posts show moderate interest, focusing on general improvements in CRISPR technology rather than the specific Cas9 variant detailed in the article.

EngagementModerate engagement

The posts demonstrate some engagement by referencing specific methods like HDR and strategies to enhance specificity, indicating an interest in technical details.

ImpactModerate impact

While the discussions highlight the technological advances in gene editing, they do not explicitly state the immediate impact of this new Cas9 variant, suggesting moderate perceived significance.

Abstract in brief

  • The researchers developed a modified version of the Cas9 enzyme called SpCas9Gem, which is designed to be broken down during the G1 phase of the cell cycle to reduce unwanted mutations from NHEJ, a process that often causes errors in gene editing.
  • They used mRNA transfection to deliver this SpCas9Gem, allowing for quick and temporary expression, which helps prevent long-term off-target effects and improves the precision of gene editing in human pluripotent stem cells.
  • This new approach significantly decreases undesired mutations at the target site while still enabling efficient creation of gene knock-ins and corrected patient-specific stem cell lines, making gene editing safer and more reliable.]

Top posts

Most liked on X and Bluesky
Genomics Gurus
@GenomicsGurus · X

PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, enzymatic base editing and the new prime editing technique for the precise genome editing with CRISPR. #CRISPR #Cas9 https://t.co/gB7AkC8dZF via @YouTube

Feb 18, 2020 · 4 likes · 2 shares · 130 followers View post →
Genomics Gurus
@GenomicsGurus · X

HOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can be used to greatly improve the specificity of the S.pyogenes CRISPR/Cas9 system during genome editing. #CRISPR #Cas9 https://t.co/bEStlEe85w via @YouTube

Feb 18, 2020 · 2 likes · 1 share · 130 followers View post →
Aggie O
@aogenetix · X

RT @GenomicsGurus: PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, en…

Feb 21, 2020 · 2 shares · 291 followers View post →
Adam West
@chromatingroup · X

RT @GenomicsGurus: HOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can…

Feb 18, 2020 · 1 share · 187 followers View post →

All mentions

Aggie O
@aogenetix · X

RT @GenomicsGurus: PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, en…

Feb 21, 2020 · 2 shares · 291 followers View post →
Genomics Gurus
@GenomicsGurus · X

PRECISION EDITING USING CRISPR In this final part of the GENE EDITING EXPLAINED! series, we describe the use of HDR, enzymatic base editing and the new prime editing technique for the precise genome editing with CRISPR. #CRISPR #Cas9 https://t.co/gB7AkC8dZF via @YouTube

Feb 18, 2020 · 4 likes · 2 shares · 130 followers View post →
Adam West
@chromatingroup · X

RT @GenomicsGurus: HOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can…

Feb 18, 2020 · 1 share · 187 followers View post →
Genomics Gurus
@GenomicsGurus · X

HOW TO IMPROVE CRISPR SPECIFICITY In part 6 of the GENE EDITING EXPLAINED! series, we describe four strategies that can be used to greatly improve the specificity of the S.pyogenes CRISPR/Cas9 system during genome editing. #CRISPR #Cas9 https://t.co/bEStlEe85w via @YouTube

Feb 18, 2020 · 2 likes · 1 share · 130 followers View post →