Volumetric Ca Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy.

Siegfried Weisenburger, Frank Tejera, Jeffrey Demas, Brandon Chen, Jason Manley, Fraser T Sparks, Francisca Martínez Traub, Tanya Daigle, Hongkui Zeng, Attila Losonczy, Alipasha Vaziri

May 2019

Synopsis of Social media discussions

The discussions reflect a generally positive reception, with several posts praising the reproducibility efforts such as including RRIDs and recognizing the article as a featured resource in a prestigious journal. Phrases like 'appreciate' and 'thank you' convey support, while mentions of the technical innovation like 'using 2P and 3P concurrently' highlight interest in the method's capabilities, suggesting that the community values the potential for advancing neuroscience research.

A
Agreement
 Moderate agreement

Most discussions express positive acknowledgment of the publication's contributions, especially highlighting the importance of reproducibility and technical advancements.

I
Interest
 High level of interest

The discussions demonstrate high interest by emphasizing the innovative aspects of HyMS microscopy and its potential applications in neuroscience.

E
Engagement
 Moderate level of engagement

Posts show moderate engagement, with some referencing specific techniques like RRID inclusion and the feature in the journal, but lacking deep analysis or critique.

I
Impact
 Moderate level of impact

Users seem to see the work as impactful within the scientific community, though the discussions center around acknowledgment rather than widespread application or change.

Social Mentions

YouTube

2 Videos

Facebook

4 Posts

Twitter

6 Posts

Blogs

6 Articles

News

14 Articles

Metrics

Video Views

416

Total Likes

9

Extended Reach

3,438

Social Features

32

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Advanced Multiplexed Light Microscopy for Neuronal Calcium Imaging in Mice

Advanced Multiplexed Light Microscopy for Neuronal Calcium Imaging in Mice

This video introduces a hybrid multiplexed sculpted light microscopy technique for calcium imaging in the mouse brain, enabling volumetric recordings at single-cell resolution across large areas at high speed in awake, behaving mice.

John Cabrera

December 17, 2020

267 views

Advanced Calcium Imaging Technique for In Vivo Neuronal Activity Analysis

Advanced Calcium Imaging Technique for In Vivo Neuronal Activity Analysis

This video discusses a new hybrid multiplexed sculpted light microscopy system that enables volumetric calcium imaging in the mouse brain, capturing activity from thousands of neurons across multiple regions with high speed and resolution.

John Cabrera

December 17, 2020

149 views

  • RRID Robot
    @RobotRrid (Twitter)

    The authors of "Volumetric Ca2+ Imaging in the Mouse Brain Using H…" (https://t.co/rf8qWwqK2t) included RRIDs in their paper! We appreciate the author's support of reproducibility. #OpenScience #RRID
    view full post

    September 5, 2021

  • RRID Robot
    @RobotRrid (Twitter)

    The authors of "untitled" (https://t.co/rf8qWw98DT) included RRIDs in their paper! Thank you for making your #methodsmatter. #reproducibility
    view full post

    August 25, 2021

  • RRID Robot
    @RobotRrid (Twitter)

    RRIDs were included in the Cell paper "untitled" (https://t.co/rf8qWw98DT). We appreciate the author's support of reproducibility. #reproducibility #ReproducibleResearch
    view full post

    August 21, 2021

  • Sigi Weisenburger
    @weisenburgers (Twitter)

    Our article about HyMS microscopy is the featured resource article in today's issue of @CellCellPress https://t.co/5d2omhahMW https://t.co/nASmTSHLLS
    view full post

    May 2, 2019

    2

  • VIB Tech Watch
    @VIBTechWatch (Twitter)

    Hybrid multiplexed sculpted light #Microscopy (HyMS) uses 2P and 3P concurrently to allow volumetric recording of neuroactivity at #SingleCell resolution! https://t.co/96NryUU6QD
    view full post

    April 18, 2019

  • Jeremy Thompson
    @thompson74d (Twitter)

    https://t.co/recC5DF4GS
    view full post

    April 16, 2019

Abstract Synopsis

  • This study introduces a new hybrid multiplexed sculpted light microscopy technique for calcium imaging in the mouse brain, overcoming traditional limitations like limited field of view, slow acquisition speed, and depth restrictions in scattering tissue.
  • The system is modular and can perform volumetric recordings at single-cell resolution within large brain areas (up to 1,122 mm³) at a high speed of 17Hz, even in awake, behaving mice.
  • The researchers demonstrated the system's capabilities by successfully recording activity from up to 12,000 neurons across various brain regions, such as the auditory cortex, posterior parietal cortex, and hippocampus, highlighting its potential for detailed in vivo neuroscience studies.]