May 2019 PMID 30982596

Volumetric Ca Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy.

Siegfried Weisenburger, Frank Tejera, Jeffrey Demas, Brandon Chen, Jason Manley, Fraser T Sparks, Francisca Martínez Traub, Tanya Daigle, Hongkui Zeng, Attila Losonczy, Alipasha Vaziri

Mentions

32
Peak: 2 in Apr 2019
across 5 sources since Apr 2019

Extended reach

3,438
potential audience

Total likes

9

Video views

416

Mentions per month

Apr 2019 – Sep 2021

Where it's discussed

  • News14 articles
  • X / Twitter6 posts
  • Blogs6 articles
  • Facebook4 posts
  • YouTube2 videos

AI synopsis

What people are saying

The discussions reflect a generally positive reception, with several posts praising the reproducibility efforts such as including RRIDs and recognizing the article as a featured resource in a prestigious journal. Phrases like 'appreciate' and 'thank you' convey support, while mentions of the technical innovation like 'using 2P and 3P concurrently' highlight interest in the method's capabilities, suggesting that the community values the potential for advancing neuroscience research.

AgreementModerate agreement

Most discussions express positive acknowledgment of the publication's contributions, especially highlighting the importance of reproducibility and technical advancements.

InterestHigh interest

The discussions demonstrate high interest by emphasizing the innovative aspects of HyMS microscopy and its potential applications in neuroscience.

EngagementModerate engagement

Posts show moderate engagement, with some referencing specific techniques like RRID inclusion and the feature in the journal, but lacking deep analysis or critique.

ImpactModerate impact

Users seem to see the work as impactful within the scientific community, though the discussions center around acknowledgment rather than widespread application or change.

Abstract in brief

  • This study introduces a new hybrid multiplexed sculpted light microscopy technique for calcium imaging in the mouse brain, overcoming traditional limitations like limited field of view, slow acquisition speed, and depth restrictions in scattering tissue.
  • The system is modular and can perform volumetric recordings at single-cell resolution within large brain areas (up to 1,122 mm³) at a high speed of 17Hz, even in awake, behaving mice.
  • The researchers demonstrated the system's capabilities by successfully recording activity from up to 12,000 neurons across various brain regions, such as the auditory cortex, posterior parietal cortex, and hippocampus, highlighting its potential for detailed in vivo neuroscience studies.]

Top posts

Most liked on X and Bluesky
Sigi Weisenburger
@weisenburgers · X

Our article about HyMS microscopy is the featured resource article in today's issue of @CellCellPress https://t.co/5d2omhahMW https://t.co/nASmTSHLLS

May 2, 2019 · 2 likes · 163 followers View post →
VIB Tech Watch
@VIBTechWatch · X

Hybrid multiplexed sculpted light #Microscopy (HyMS) uses 2P and 3P concurrently to allow volumetric recording of neuroactivity at #SingleCell resolution! https://t.co/96NryUU6QD

Apr 18, 2019 · 2,088 followers View post →
Jeremy Thompson
@thompson74d · X

https://t.co/recC5DF4GS

Apr 16, 2019 · 289 followers View post →
RRID Robot
@RobotRrid · X

The authors of "Volumetric Ca2+ Imaging in the Mouse Brain Using H…" (https://t.co/rf8qWwqK2t) included RRIDs in their paper! We appreciate the author's support of reproducibility. #OpenScience #RRID

Sep 5, 2021 · 161 followers View post →
RRID Robot
@RobotRrid · X

The authors of "untitled" (https://t.co/rf8qWw98DT) included RRIDs in their paper! Thank you for making your #methodsmatter. #reproducibility

Aug 25, 2021 · 161 followers View post →
RRID Robot
@RobotRrid · X

RRIDs were included in the Cell paper "untitled" (https://t.co/rf8qWw98DT). We appreciate the author's support of reproducibility. #reproducibility #ReproducibleResearch

Aug 21, 2021 · 161 followers View post →

All mentions

RRID Robot
@RobotRrid · X

The authors of "Volumetric Ca2+ Imaging in the Mouse Brain Using H…" (https://t.co/rf8qWwqK2t) included RRIDs in their paper! We appreciate the author's support of reproducibility. #OpenScience #RRID

Sep 5, 2021 · 161 followers View post →
RRID Robot
@RobotRrid · X

The authors of "untitled" (https://t.co/rf8qWw98DT) included RRIDs in their paper! Thank you for making your #methodsmatter. #reproducibility

Aug 25, 2021 · 161 followers View post →
RRID Robot
@RobotRrid · X

RRIDs were included in the Cell paper "untitled" (https://t.co/rf8qWw98DT). We appreciate the author's support of reproducibility. #reproducibility #ReproducibleResearch

Aug 21, 2021 · 161 followers View post →
Sigi Weisenburger
@weisenburgers · X

Our article about HyMS microscopy is the featured resource article in today's issue of @CellCellPress https://t.co/5d2omhahMW https://t.co/nASmTSHLLS

May 2, 2019 · 2 likes · 163 followers View post →
VIB Tech Watch
@VIBTechWatch · X

Hybrid multiplexed sculpted light #Microscopy (HyMS) uses 2P and 3P concurrently to allow volumetric recording of neuroactivity at #SingleCell resolution! https://t.co/96NryUU6QD

Apr 18, 2019 · 2,088 followers View post →
Jeremy Thompson
@thompson74d · X

https://t.co/recC5DF4GS

Apr 16, 2019 · 289 followers View post →