Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers.

Harrison Specht, Nikolai Slavov

January 2021

Synopsis of Social media discussions

The discussions reflect strong support and excitement about the article, with mentions of its role in advancing single-cell proteomics and practical guidelines, illustrating high relevance through phrases like 'great results,' 'optimization,' and 'guidelines.' The tone is enthusiastic and detailed, emphasizing the research's potential to influence experimental approaches and scientific understanding.

A
Agreement
 Moderate agreement

Most discussions highlight the positive impact and support for the article, emphasizing its practical benefits and validation studies.

I
Interest
 High level of interest

Posts show high curiosity and enthusiasm, especially surrounding advancements in single-cell proteomics and optimization guidelines.

E
Engagement
 High engagement

Many comments delve into specific methods, trade-offs, and experimental details, indicating deep engagement.

I
Impact
 High level of impact

The discussions suggest the research has significant implications for improving proteomics techniques and facilitating future studies.

Social Mentions

YouTube

5 Videos

Facebook

2 Posts

Twitter

64 Posts

Blogs

4 Articles

News

2 Articles

Metrics

Video Views

4,941

Total Likes

229

Extended Reach

580,635

Social Features

77

Timeline: Posts about article

Top Social Media Posts

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  • Rob Townley
    @RobTownley6 (Twitter)

    RT @slavov_n: Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant i…
    view full post

    April 12, 2024

    2

  • Akila Wijerathna-Yapa
    @AkilaWijerathna (Twitter)

    RT @slavov_n: Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant i…
    view full post

    April 12, 2024

    2

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant in 2024. https://t.co/JPAMlORLMC
    view full post

    April 12, 2024

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    2

  • Greg Elsden
    @ElsdenGregory (Twitter)

    RT @SCP_meeting: The talk by Luke Koury at #SCP2023 is on YouTube: Single-cell proteomics with new mass spectrometry platforms https://t…
    view full post

    July 25, 2023

    1

  • Single-Cell Proteomics Conference
    @SCP_meeting (Twitter)

    The talk by Luke Koury at #SCP2023 is on YouTube: Single-cell proteomics with new mass spectrometry platforms https://t.co/FIZ2Av0v8w
    view full post

    July 19, 2023

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  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    @AlfaroJav About the same number of peptide like features as now we can detect from a single human cell. https://t.co/LKOq5CisAh
    view full post

    February 15, 2023

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  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    To me, it all seemed simple and logical. As the field advanced, it further clarified. It became clear that no stochastic resonance is needed (or happening), and precursor detection is easy, even for old MS instruments. https://t.co/LKOq5Cj0pP https://t.co/GsWhrpecBD
    view full post

    November 15, 2022

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    The analysis of a single Hela cell by mass-spec identifies over 60,000 peptide-like features. https://t.co/LKOq5CisAh https://t.co/QI5DCK3FQx
    view full post

    October 2, 2021

  • Mass-spec@RIKEN BDR KOBE
    @bdr_massspec (Twitter)

    RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
    view full post

    June 13, 2021

    6

  • Mohd Altaf Najar, Ph.D.
    @MohammadAltafN3 (Twitter)

    RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
    view full post

    June 12, 2021

    6

  • BreakthroughMedicine&AdvancedResearchTools
    @JayChance12 (Twitter)

    RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
    view full post

    June 12, 2021

    6

  • Kathirvel Alagesan
    @kathiralagesan (Twitter)

    RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
    view full post

    June 12, 2021

    6

  • Andrea Petretto
    @Angrist_Fusion (Twitter)

    RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
    view full post

    June 12, 2021

    6

  • Prof(ret.) Dr Rune Linding 
    @RuneLinding (Twitter)

    RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
    view full post

    June 12, 2021

    6

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureComms Consistent with prior observations, the isobaric carrier increased sensitivity without adversely affecting quantification https://t.co/LKOq5CisAh https://t.co/eKaBqSYcCf
    view full post

    June 12, 2021

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  • Northeastern University Bioengineering
    @NUBioE1 (Twitter)

    RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
    view full post

    February 1, 2021

    3

  • Sara Fonseca Costa
    @essepf (Twitter)

    RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
    view full post

    January 14, 2021

    3

  • Ed Emmott
    @edemmott (Twitter)

    RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
    view full post

    January 14, 2021

    3

  • Journal of Proteome Research
    @JProteomeRes (Twitter)

    RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
    view full post

    January 14, 2021

    3

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved Harrison! Check out some of his @JProteomeRes papers: https://t.co/LKOq5CA3rP https://t.co/RfjJHYV6fA https://t.co/peylTwJdyY
    view full post

    January 14, 2021

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  • Slavov Laboratory
    @slavovLab (Twitter)

    RT @AllenInstitute: Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Qu…
    view full post

    January 11, 2021

    5

  • Kathryn Richmond
    @DrKathyRichmond (Twitter)

    RT @AllenInstitute: Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Qu…
    view full post

    January 8, 2021

    5

  • Allen Institute
    @AllenInstitute (Twitter)

    Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers" Read: https://t.co/InyH1pDMvg https://t.co/A6sIIWYHyR
    view full post

    January 8, 2021

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  • COLLINS MALLACS
    @CMallacs (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    January 4, 2021

    10

  • COLLINS MALLACS
    @CMallacs (Twitter)

    RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
    view full post

    January 1, 2021

    4

  • lbgbox
    @lbgbox (Twitter)

    RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
    view full post

    January 1, 2021

    4

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
    view full post

    December 31, 2020

    4

  • Mass Spec Pro
    @massspecpro (Twitter)

    RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
    view full post

    December 31, 2020

    4

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution https://t.co/LKOq5CA3rP, though it often comes at the expense of analyzing fewer proteins. https://t.co/3M8gtUalfk
    view full post

    December 31, 2020

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  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    Yes, in my experience peer review at @JProteomeRes has been critical, thoughtful & constructive. Also, @ACSPublications perfectly typeset our paper https://t.co/LKOq5CA3rP in just 2 weeks starting with the latex source. The paper needed no corrections! https://t.co/bLLHccFZ6F
    view full post

    December 16, 2020

    3

  • lbgbox
    @lbgbox (Twitter)

    RT @slavov_n: Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align wi…
    view full post

    December 11, 2020

    1

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align with previously discussed & demonstrated effects https://t.co/LKOq5CisAh https://t.co/RfjJHYDvo2 https://t.co/K78qaokhUY
    view full post

    December 10, 2020

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  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    @JohnRYatesIII @Sci_j_my It depends on the platform used (the latest platforms don't necessarily increase depth) & on the cell type, e.g., # RNAs detected in neurons or Hela cells >> monocytes. Most reports focus on # of genes detected. I gave an overview at the @SCP_meeting https://t.co/liqblWCecz https://t.co/7uQFjpbj5I
    view full post

    November 22, 2020

    2

  • Single Cell papers
    @scell_papers (Twitter)

    Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers. https://t.co/RbUHAvIIol
    view full post

    November 18, 2020

    1

  • Pastel BioScience
    @PastelBio (Twitter)

    Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #proteomics https://t.co/iJ0Kcb63JC
    view full post

    November 18, 2020

    4

  • Prof(ret.) Dr Rune Linding 
    @RuneLinding (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 17, 2020

    10

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 17, 2020

    10

  • Emmanuel Ríos Castro
    @qfb_erc (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 16, 2020

    10

  • Andrew Leduc
    @_AndrewLeduc (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 16, 2020

    10

  • Mass-spec@RIKEN BDR KOBE
    @bdr_massspec (Twitter)

    RT @PastelBio_prot: [ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/w1Q6…
    view full post

    November 16, 2020

    1

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 16, 2020

    10

  • Mass-spec@RIKEN BDR KOBE
    @bdr_massspec (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 16, 2020

    10

  • Aedin Culhane (@AedinCulhane@genomic.social)
    @AedinCulhane (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 16, 2020

    10

  • Ed Emmott
    @edemmott (Twitter)

    RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
    view full post

    November 16, 2020

    10

  • Slavov Laboratory
    @slavovLab (Twitter)

    Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and suggests guidelines reflecting project priorities https://t.co/wWXHIhu96J https://t.co/6xVuF0SADG
    view full post

    November 16, 2020

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  • Journal of Proteome Research
    @JProteomeRes (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 16, 2020

    8

  • Mass-spec@RIKEN BDR KOBE
    @bdr_massspec (Twitter)

    RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…
    view full post

    November 15, 2020

    3

  • Giovani Veríssimo
    @Giovaniverissim (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 15, 2020

    8

  • Pastel BioScience
    @PastelBio (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 15, 2020

    8

  • Giovani Veríssimo
    @Giovaniverissim (Twitter)

    RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…
    view full post

    November 15, 2020

    3

  • nextgenseek
    @nextgenseek (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 15, 2020

    8

  • Bharath Kumar Raghuraman
    @rbharathkumar91 (Twitter)

    RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…
    view full post

    November 15, 2020

    3

  • Arjun Raj
    @arjunrajlab (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 15, 2020

    8

  • Pastel BioScience
    @PastelBio (Twitter)

    Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #proteomics @slavov_n https://t.co/GlR7NWbCJn
    view full post

    November 15, 2020

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  • Dr. Biswapriya Misra
    @BiswapriyaMisra (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 15, 2020

    8

  • Edu Chicano (@educhicano@bsky.social)
    @educhicano (Twitter)

    "[ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers" https://t.co/BPc9IhR5jp #proteomics
    view full post

    November 14, 2020

  • Pastel BioScience
    @PastelBio_prot (Twitter)

    [ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/w1Q6nqLLOz
    view full post

    November 14, 2020

    1

    1

  • MyJournals
    @myjournals (Twitter)

    [ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/16li4j77AD
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    November 14, 2020

  • Willi
    @willigo09 (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 14, 2020

    8

  • Gholson Lyon
    @GholsonLyon (Twitter)

    RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
    view full post

    November 14, 2020

    8

  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric carriers. Peer review at @JProteomeRes was constructive & fast: https://t.co/LKOq5CisAh Here is the #OA version: https://t.co/MieXs6sqYP Thread
    view full post

    November 14, 2020

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  • Single-Cell Proteomics Conference
    @SCP_meeting (Twitter)

    Single-cell analysis of proteins and RNAs: Optimizing reliability, depth, and throughput A talk by Nikolai Slavov (@slavov_n) at #SCP2020 https://t.co/gmfOLARKzQ
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    September 10, 2020

    2

  • Mitochondrial News
    @MitochondriaNe1 (Twitter)

    Optimizing accuracy and depth of protein quantification in experiments using isobaric carriers https://t.co/98T7knglKc
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    August 24, 2020

  • bioRxiv
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    Optimizing accuracy and depth of protein quantification in experiments using isobaric carriers https://t.co/Gtm6ChlHtJ #bioRxiv
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    August 24, 2020

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Abstract Synopsis

  • The isobaric carrier method combines small labeled samples with a larger carrier to improve peptide identification in mass spectrometry, especially for tiny samples like single cells, without increasing the actual number of proteins sampled.
  • The study highlights key tradeoffs when using isobaric carriers, such as enhancing peptide identification versus the accuracy of protein quantification, and suggests ways to optimize experimental parameters like carrier amount and ion accumulation time to suit specific research goals.
  • These insights help tailor experiments for various applications—like analyzing limited tissue samples, studying single-cell proteomes, or mapping protein interactions—while emphasizing the importance of estimating and considering quantification errors in data analysis.]