I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric carriers. Peer review at @JProteomeRes was constructive & fast: https://t.co/LKOq5CisAh Here is the #OA version: https://t.co/MieXs6sqYP Thread
Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers.
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Aug 2020 – Apr 2024Where it's discussed
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What people are saying
The discussions reflect strong support and excitement about the article, with mentions of its role in advancing single-cell proteomics and practical guidelines, illustrating high relevance through phrases like 'great results,' 'optimization,' and 'guidelines.' The tone is enthusiastic and detailed, emphasizing the research's potential to influence experimental approaches and scientific understanding.
Most discussions highlight the positive impact and support for the article, emphasizing its practical benefits and validation studies.
Posts show high curiosity and enthusiasm, especially surrounding advancements in single-cell proteomics and optimization guidelines.
Many comments delve into specific methods, trade-offs, and experimental details, indicating deep engagement.
The discussions suggest the research has significant implications for improving proteomics techniques and facilitating future studies.
Abstract in brief
- The isobaric carrier method combines small labeled samples with a larger carrier to improve peptide identification in mass spectrometry, especially for tiny samples like single cells, without increasing the actual number of proteins sampled.
- The study highlights key tradeoffs when using isobaric carriers, such as enhancing peptide identification versus the accuracy of protein quantification, and suggests ways to optimize experimental parameters like carrier amount and ion accumulation time to suit specific research goals.
- These insights help tailor experiments for various applications—like analyzing limited tissue samples, studying single-cell proteomes, or mapping protein interactions—while emphasizing the importance of estimating and considering quantification errors in data analysis.]
Top posts
Most liked on X and BlueskySingle-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and suggests guidelines reflecting project priorities https://t.co/wWXHIhu96J https://t.co/6xVuF0SADG
The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureComms Consistent with prior observations, the isobaric carrier increased sensitivity without adversely affecting quantification https://t.co/LKOq5CisAh https://t.co/eKaBqSYcCf
Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved Harrison! Check out some of his @JProteomeRes papers: https://t.co/LKOq5CA3rP https://t.co/RfjJHYV6fA https://t.co/peylTwJdyY
I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution https://t.co/LKOq5CA3rP, though it often comes at the expense of analyzing fewer proteins. https://t.co/3M8gtUalfk
Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align with previously discussed & demonstrated effects https://t.co/LKOq5CisAh https://t.co/RfjJHYDvo2 https://t.co/K78qaokhUY
All mentions
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Advancing Single-Cell Proteomics with Innovative Techniques
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Luke Koury discusses recent developments in single-cell proteomics at SCP2023, highlighting new mass spectrometry platforms and best practices for improving accuracy and reproducibility in protein analysis from…
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RT @slavov_n: Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant i…
RT @slavov_n: Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant i…
Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant in 2024. https://t.co/JPAMlORLMC
RT @SCP_meeting: The talk by Luke Koury at #SCP2023 is on YouTube: Single-cell proteomics with new mass spectrometry platforms https://t…
The talk by Luke Koury at #SCP2023 is on YouTube: Single-cell proteomics with new mass spectrometry platforms https://t.co/FIZ2Av0v8w
@AlfaroJav About the same number of peptide like features as now we can detect from a single human cell. https://t.co/LKOq5CisAh
To me, it all seemed simple and logical. As the field advanced, it further clarified. It became clear that no stochastic resonance is needed (or happening), and precursor detection is easy, even for old MS instruments. https://t.co/LKOq5Cj0pP https://t.co/GsWhrpecBD
The analysis of a single Hela cell by mass-spec identifies over 60,000 peptide-like features. https://t.co/LKOq5CisAh https://t.co/QI5DCK3FQx
RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…
The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureComms Consistent with prior observations, the isobaric carrier increased sensitivity without adversely affecting quantification https://t.co/LKOq5CisAh https://t.co/eKaBqSYcCf
RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…
Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved Harrison! Check out some of his @JProteomeRes papers: https://t.co/LKOq5CA3rP https://t.co/RfjJHYV6fA https://t.co/peylTwJdyY
RT @AllenInstitute: Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Qu…
RT @AllenInstitute: Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Qu…
Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers" Read: https://t.co/InyH1pDMvg https://t.co/A6sIIWYHyR
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…
I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution https://t.co/LKOq5CA3rP, though it often comes at the expense of analyzing fewer proteins. https://t.co/3M8gtUalfk
Yes, in my experience peer review at @JProteomeRes has been critical, thoughtful & constructive. Also, @ACSPublications perfectly typeset our paper https://t.co/LKOq5CA3rP in just 2 weeks starting with the latex source. The paper needed no corrections! https://t.co/bLLHccFZ6F
RT @slavov_n: Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align wi…
Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align with previously discussed & demonstrated effects https://t.co/LKOq5CisAh https://t.co/RfjJHYDvo2 https://t.co/K78qaokhUY
@JohnRYatesIII @Sci_j_my It depends on the platform used (the latest platforms don't necessarily increase depth) & on the cell type, e.g., # RNAs detected in neurons or Hela cells >> monocytes. Most reports focus on # of genes detected. I gave an overview at the @SCP_meeting https://t.co/liqblWCecz https://t.co/7uQFjpbj5I
Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers. https://t.co/RbUHAvIIol
Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #proteomics https://t.co/iJ0Kcb63JC
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @PastelBio_prot: [ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/w1Q6…
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…
Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and suggests guidelines reflecting project priorities https://t.co/wWXHIhu96J https://t.co/6xVuF0SADG
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #proteomics @slavov_n https://t.co/GlR7NWbCJn
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
"[ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers" https://t.co/BPc9IhR5jp #proteomics
[ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/w1Q6nqLLOz
[ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/16li4j77AD
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…
I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric carriers. Peer review at @JProteomeRes was constructive & fast: https://t.co/LKOq5CisAh Here is the #OA version: https://t.co/MieXs6sqYP Thread
Single-cell analysis of proteins and RNAs: Optimizing reliability, depth, and throughput A talk by Nikolai Slavov (@slavov_n) at #SCP2020 https://t.co/gmfOLARKzQ
Optimizing accuracy and depth of protein quantification in experiments using isobaric carriers https://t.co/98T7knglKc
Optimizing accuracy and depth of protein quantification in experiments using isobaric carriers https://t.co/Gtm6ChlHtJ #bioRxiv