January 2021 PMID 33190502

Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers.

Harrison Specht, Nikolai Slavov

Mentions

77
Peak: 29 in Nov 2020
across 5 sources since Aug 2020

Extended reach

580,635
potential audience

Total likes

229

Video views

4,941

Mentions per month

Aug 2020 – Apr 2024

Where it's discussed

  • X / Twitter64 posts
  • YouTube5 videos
  • Blogs4 articles
  • Facebook2 posts
  • News2 articles

AI synopsis

What people are saying

The discussions reflect strong support and excitement about the article, with mentions of its role in advancing single-cell proteomics and practical guidelines, illustrating high relevance through phrases like 'great results,' 'optimization,' and 'guidelines.' The tone is enthusiastic and detailed, emphasizing the research's potential to influence experimental approaches and scientific understanding.

AgreementModerate agreement

Most discussions highlight the positive impact and support for the article, emphasizing its practical benefits and validation studies.

InterestHigh interest

Posts show high curiosity and enthusiasm, especially surrounding advancements in single-cell proteomics and optimization guidelines.

EngagementHigh engagement

Many comments delve into specific methods, trade-offs, and experimental details, indicating deep engagement.

ImpactHigh impact

The discussions suggest the research has significant implications for improving proteomics techniques and facilitating future studies.

Abstract in brief

  • The isobaric carrier method combines small labeled samples with a larger carrier to improve peptide identification in mass spectrometry, especially for tiny samples like single cells, without increasing the actual number of proteins sampled.
  • The study highlights key tradeoffs when using isobaric carriers, such as enhancing peptide identification versus the accuracy of protein quantification, and suggests ways to optimize experimental parameters like carrier amount and ion accumulation time to suit specific research goals.
  • These insights help tailor experiments for various applications—like analyzing limited tissue samples, studying single-cell proteomes, or mapping protein interactions—while emphasizing the importance of estimating and considering quantification errors in data analysis.]

Top posts

Most liked on X and Bluesky
Prof. Nikolai Slavov
@slavov_n · X

I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric carriers. Peer review at @JProteomeRes was constructive & fast: https://t.co/LKOq5CisAh Here is the #OA version: https://t.co/MieXs6sqYP Thread

Nov 14, 2020 · 29 likes · 8 shares · 19,979 followers View post →
Slavov Laboratory
@slavovLab · X

Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and suggests guidelines reflecting project priorities https://t.co/wWXHIhu96J https://t.co/6xVuF0SADG

Nov 16, 2020 · 25 likes · 10 shares · 2,831 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureComms Consistent with prior observations, the isobaric carrier increased sensitivity without adversely affecting quantification https://t.co/LKOq5CisAh https://t.co/eKaBqSYcCf

Jun 12, 2021 · 22 likes · 6 shares · 19,979 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved Harrison! Check out some of his @JProteomeRes papers: https://t.co/LKOq5CA3rP https://t.co/RfjJHYV6fA https://t.co/peylTwJdyY

Jan 14, 2021 · 19 likes · 3 shares · 19,979 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution https://t.co/LKOq5CA3rP, though it often comes at the expense of analyzing fewer proteins. https://t.co/3M8gtUalfk

Dec 31, 2020 · 16 likes · 4 shares · 19,979 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align with previously discussed & demonstrated effects https://t.co/LKOq5CisAh https://t.co/RfjJHYDvo2 https://t.co/K78qaokhUY

Dec 10, 2020 · 15 likes · 1 share · 19,979 followers View post →

All mentions

Rob Townley
@RobTownley6 · X

RT @slavov_n: Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant i…

Apr 12, 2024 · 2 shares · 189 followers View post →
Akila Wijerathna-Yapa
@AkilaWijerathna · X

RT @slavov_n: Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant i…

Apr 12, 2024 · 2 shares · 918 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

Today I recalled my talk from SCP2020. In some ways, it reflects a distant era. In other ways, it remains just as relevant in 2024. https://t.co/JPAMlORLMC

Apr 12, 2024 · 2 likes · 2 shares · 19,933 followers View post →
Greg Elsden
@ElsdenGregory · X

RT @SCP_meeting: The talk by Luke Koury at #SCP2023 is on YouTube: Single-cell proteomics with new mass spectrometry platforms https://t…

Jul 25, 2023 · 1 share · 7 followers View post →
Single-Cell Proteomics Conference
@SCP_meeting · X

The talk by Luke Koury at #SCP2023 is on YouTube: Single-cell proteomics with new mass spectrometry platforms https://t.co/FIZ2Av0v8w

Jul 19, 2023 · 2 likes · 1 share · 2,207 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

@AlfaroJav About the same number of peptide like features as now we can detect from a single human cell. https://t.co/LKOq5CisAh

Feb 15, 2023 · 4 likes · 1 share · 19,979 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

To me, it all seemed simple and logical. As the field advanced, it further clarified. It became clear that no stochastic resonance is needed (or happening), and precursor detection is easy, even for old MS instruments. https://t.co/LKOq5Cj0pP https://t.co/GsWhrpecBD

Nov 15, 2022 · 19,979 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

The analysis of a single Hela cell by mass-spec identifies over 60,000 peptide-like features. https://t.co/LKOq5CisAh https://t.co/QI5DCK3FQx

Oct 2, 2021 · 19,979 followers View post →
Mass-spec@RIKEN BDR KOBE
@bdr_massspec · X

RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…

Jun 13, 2021 · 6 shares · 68 followers View post →
Mohd Altaf Najar, Ph.D.
@MohammadAltafN3 · X

RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…

Jun 12, 2021 · 6 shares · 438 followers View post →
BreakthroughMedicine&AdvancedResearchTools
@JayChance12 · X

RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…

Jun 12, 2021 · 6 shares · 620 followers View post →
Kathirvel Alagesan
@kathiralagesan · X

RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…

Jun 12, 2021 · 6 shares · 1,041 followers View post →
Andrea Petretto
@Angrist_Fusion · X

RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…

Jun 12, 2021 · 6 shares · 580 followers View post →
Prof(ret.) Dr Rune Linding 
@RuneLinding · X

RT @slavov_n: The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureC…

Jun 12, 2021 · 6 shares · 3,056 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

The isobaric carrier enabled microscaled analysis of clinical samples with great results https://t.co/qivHlvdxL8 via @NatureComms Consistent with prior observations, the isobaric carrier increased sensitivity without adversely affecting quantification https://t.co/LKOq5CisAh https://t.co/eKaBqSYcCf

Jun 12, 2021 · 22 likes · 6 shares · 19,979 followers View post →
Northeastern University Bioengineering
@NUBioE1 · X

RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…

Feb 1, 2021 · 3 shares · 613 followers View post →
Sara Fonseca Costa
@essepf · X

RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…

Jan 14, 2021 · 3 shares · 253 followers View post →
Ed Emmott
@edemmott · X

RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…

Jan 14, 2021 · 3 shares · 3,728 followers View post →
Journal of Proteome Research
@JProteomeRes · X

RT @slavov_n: Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved H…

Jan 14, 2021 · 3 shares · 3,826 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

Congratulation to @harrisonspecht for being selected a rising start by @JProteomeRes https://t.co/iVnJZFazgg Well deserved Harrison! Check out some of his @JProteomeRes papers: https://t.co/LKOq5CA3rP https://t.co/RfjJHYV6fA https://t.co/peylTwJdyY

Jan 14, 2021 · 19 likes · 3 shares · 19,979 followers View post →
Slavov Laboratory
@slavovLab · X

RT @AllenInstitute: Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Qu…

Jan 11, 2021 · 5 shares · 2,831 followers View post →
Kathryn Richmond
@DrKathyRichmond · X

RT @AllenInstitute: Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Qu…

Jan 8, 2021 · 5 shares · 1,424 followers View post →
Allen Institute
@AllenInstitute · X

Check out this latest study by Allen Distinguished Investigator @slavov_n: "Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers" Read: https://t.co/InyH1pDMvg https://t.co/A6sIIWYHyR

Jan 8, 2021 · 11 likes · 5 shares · 66,427 followers View post →
COLLINS MALLACS
@CMallacs · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Jan 4, 2021 · 10 shares · 172 followers View post →
COLLINS MALLACS
@CMallacs · X

RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…

Jan 1, 2021 · 4 shares · 172 followers View post →
lbgbox
@lbgbox · X

RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…

Jan 1, 2021 · 4 shares · 51 followers View post →
Single Cell Media
@SingleCellMedia · X

RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…

Dec 31, 2020 · 4 shares · 3,837 followers View post →
Mass Spec Pro
@massspecpro · X

RT @slavov_n: I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution…

Dec 31, 2020 · 4 shares · 4,346 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

I am often asked about increasing the sensitivity of single-cell protein analysis by mass-spec. Here is one simple solution https://t.co/LKOq5CA3rP, though it often comes at the expense of analyzing fewer proteins. https://t.co/3M8gtUalfk

Dec 31, 2020 · 16 likes · 4 shares · 19,979 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

Yes, in my experience peer review at @JProteomeRes has been critical, thoughtful & constructive. Also, @ACSPublications perfectly typeset our paper https://t.co/LKOq5CA3rP in just 2 weeks starting with the latex source. The paper needed no corrections! https://t.co/bLLHccFZ6F

Dec 16, 2020 · 3 likes · 19,979 followers View post →
lbgbox
@lbgbox · X

RT @slavov_n: Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align wi…

Dec 11, 2020 · 1 share · 51 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

Another benchmarking study of the isobaric carrier in @naturemethods https://t.co/fYFGviN3Ch. The main conclusions align with previously discussed & demonstrated effects https://t.co/LKOq5CisAh https://t.co/RfjJHYDvo2 https://t.co/K78qaokhUY

Dec 10, 2020 · 15 likes · 1 share · 19,979 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

@JohnRYatesIII @Sci_j_my It depends on the platform used (the latest platforms don't necessarily increase depth) & on the cell type, e.g., # RNAs detected in neurons or Hela cells >> monocytes. Most reports focus on # of genes detected. I gave an overview at the @SCP_meeting https://t.co/liqblWCecz https://t.co/7uQFjpbj5I

Nov 22, 2020 · 2 likes · 19,933 followers View post →
Single Cell papers
@scell_papers · X

Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers. https://t.co/RbUHAvIIol

Nov 18, 2020 · 1 like · 11,517 followers View post →
Pastel BioScience
@PastelBio · X

Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #proteomics https://t.co/iJ0Kcb63JC

Nov 18, 2020 · 4 likes · 5,375 followers View post →
Prof(ret.) Dr Rune Linding 
@RuneLinding · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 17, 2020 · 10 shares · 3,056 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 17, 2020 · 10 shares · 19,979 followers View post →
Emmanuel Ríos Castro
@qfb_erc · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 16, 2020 · 10 shares · 512 followers View post →
Andrew Leduc
@_AndrewLeduc · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 16, 2020 · 10 shares · 616 followers View post →
Mass-spec@RIKEN BDR KOBE
@bdr_massspec · X

RT @PastelBio_prot: [ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/w1Q6…

Nov 16, 2020 · 1 share · 68 followers View post →
Single Cell Media
@SingleCellMedia · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 16, 2020 · 10 shares · 3,837 followers View post →
Mass-spec@RIKEN BDR KOBE
@bdr_massspec · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 16, 2020 · 10 shares · 68 followers View post →
Aedin Culhane (@AedinCulhane@genomic.social)
@AedinCulhane · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 16, 2020 · 10 shares · 3,785 followers View post →
Ed Emmott
@edemmott · X

RT @slavovLab: Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and sug…

Nov 16, 2020 · 10 shares · 3,728 followers View post →
Slavov Laboratory
@slavovLab · X

Single-cell proteomics analysis needs careful optimization. Our study led by @harrisonspecht demonstrates trade-offs and suggests guidelines reflecting project priorities https://t.co/wWXHIhu96J https://t.co/6xVuF0SADG

Nov 16, 2020 · 25 likes · 10 shares · 2,831 followers View post →
Journal of Proteome Research
@JProteomeRes · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 16, 2020 · 8 shares · 3,826 followers View post →
Mass-spec@RIKEN BDR KOBE
@bdr_massspec · X

RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…

Nov 15, 2020 · 3 shares · 68 followers View post →
Giovani Veríssimo
@Giovaniverissim · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 15, 2020 · 8 shares · 65 followers View post →
Pastel BioScience
@PastelBio · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 15, 2020 · 8 shares · 5,375 followers View post →
Giovani Veríssimo
@Giovaniverissim · X

RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…

Nov 15, 2020 · 3 shares · 65 followers View post →
nextgenseek
@nextgenseek · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 15, 2020 · 8 shares · 6,361 followers View post →
Bharath Kumar Raghuraman
@rbharathkumar91 · X

RT @PastelBio: Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #pr…

Nov 15, 2020 · 3 shares · 294 followers View post →
Arjun Raj
@arjunrajlab · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 15, 2020 · 8 shares · 27,486 followers View post →
Pastel BioScience
@PastelBio · X

Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers | https://t.co/EkLsVYQfwO #proteomics @slavov_n https://t.co/GlR7NWbCJn

Nov 15, 2020 · 7 likes · 3 shares · 5,375 followers View post →
Dr. Biswapriya Misra
@BiswapriyaMisra · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 15, 2020 · 8 shares · 5,765 followers View post →
Edu Chicano (@educhicano@bsky.social)
@educhicano · X

"[ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers" https://t.co/BPc9IhR5jp #proteomics

Nov 14, 2020 · 1,349 followers View post →
Pastel BioScience
@PastelBio_prot · X

[ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/w1Q6nqLLOz

Nov 14, 2020 · 1 like · 1 share · 215 followers View post →
MyJournals
@myjournals · X

[ASAP] Optimizing Accuracy and Depth of Protein Quantification in Experiments Using Isobaric Carriers https://t.co/16li4j77AD

Nov 14, 2020 · 557 followers View post →
Willi
@willigo09 · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 14, 2020 · 8 shares · 204 followers View post →
Gholson Lyon
@GholsonLyon · X

RT @slavov_n: I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric car…

Nov 14, 2020 · 8 shares · 4,885 followers View post →
Prof. Nikolai Slavov
@slavov_n · X

I very much enjoyed writing this paper, from the concepts and controlled experiments to the guidelines for using isobaric carriers. Peer review at @JProteomeRes was constructive & fast: https://t.co/LKOq5CisAh Here is the #OA version: https://t.co/MieXs6sqYP Thread

Nov 14, 2020 · 29 likes · 8 shares · 19,979 followers View post →
Single-Cell Proteomics Conference
@SCP_meeting · X

Single-cell analysis of proteins and RNAs: Optimizing reliability, depth, and throughput A talk by Nikolai Slavov (@slavov_n) at #SCP2020 https://t.co/gmfOLARKzQ

Sep 10, 2020 · 2 likes · 2,207 followers View post →
Mitochondrial News
@MitochondriaNe1 · X

Optimizing accuracy and depth of protein quantification in experiments using isobaric carriers https://t.co/98T7knglKc

Aug 24, 2020 · 2,255 followers View post →
bioRxiv
@biorxivpreprint · X

Optimizing accuracy and depth of protein quantification in experiments using isobaric carriers https://t.co/Gtm6ChlHtJ #bioRxiv

Aug 24, 2020 · 2 likes · 1 share · 136,860 followers View post →