ACS Synth Biol March 2022 PMID 35259873

Constructing Cell-Free Expression Systems for Low-Cost Access.

Fernando Guzman-Chavez, Anibal Arce, Abhinav Adhikari, Sandra Vadhin, Jose Antonio Pedroza-Garcia, Chiara Gandini, Jim W Ajioka, Jenny Molloy, Sobeida Sanchez-Nieto, Jeffrey D Varner, Fernan Federici, Jim Haseloff

Mentions

81
Peak: 44 in Mar 2022
across 5 sources since Mar 2022

Extended reach

311,224
potential audience

Total likes

299

Video views

233

Mentions per month

Mar 2022 – Apr 2025

Where it's discussed

  • X / Twitter75 posts
  • YouTube2 videos
  • News2 articles
  • Facebook1 posts
  • LinkedIn1 posts

AI synopsis

What people are saying

The discussions consistently highlight the innovative nature of the work, such as developing dry, cold-chain-free systems and lowering costs by up to 24 times, using words like 'exciting' and 'game-changer.' Participants actively share practical ideas, like drying reactions with desiccators, showing both high interest and engagement, underscoring the perceived broad impact on global biotechnology access.

AgreementModerate agreement

Most discussions support the significance and potential of the research, highlighting its innovative approaches and practical benefits.

InterestHigh interest

Multiple posts show enthusiasm by expressing eagerness to try the methods and attend talks, indicating high curiosity.

EngagementHigh engagement

Posts delve into technical details like drying reactions and reducing costs, demonstrating deep engagement with the subject.

ImpactHigh impact

The discussions emphasize how the research could transform low-cost biotech applications, especially in low- and middle-income countries, indicating high perceived impact.

Abstract in brief

  • The article discusses developing low-cost, accessible cell-free gene expression systems by modifying existing methods, such as using silica beads for drying reactions and adding lactose to boost protein production.
  • Researchers demonstrated the endogenous regeneration of key molecules like nucleoside triphosphates within the system, reducing the need for expensive external reagents.
  • These innovations significantly lower costs—by up to 24 times—making cell-free systems more available for research and education, especially in low- and middle-income countries.]

Top posts

Most liked on X and Bluesky
Sebastian S. Cocioba
@ATinyGreenCell · X

How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 113 likes · 16 shares · 31,310 followers View post →
Fernando
@MrSorbicillin · X

Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without adding any exogenous NTPs?... These and more information here https://t.co/0EkpomkMAt

Mar 8, 2022 · 110 likes · 35 shares · 195 followers View post →
Open Bioeconomy Lab
@openbioeconomy · X

Upcoming: An exciting talk on Constructing Cell-Free Expression Systems for Low-Cost Access with Dr Fernando Guzman-Chavez and Open Bioeconomy Lab. Join us on Wed 27 July, 12:00-13:00 @ LT3, CEB & online via Zoom RSVP https://t.co/jYydfMG79y #synbio #biotech #biomanufacturing https://t.co/o3eVQG3Gp7

Jul 25, 2022 · 15 likes · 4 shares · 1,969 followers View post →
Leonardo Rios
@DrLeoRios · X

Low cost cell free systems #synbio

Mar 11, 2022 · 14 likes · 4 shares · 9,352 followers View post →
Open Bioeconomy Lab
@openbioeconomy · X

Critical points from our talk with Dr Fernando Guzman on Constructing Cell-Free Expression Systems for Low-Cost Access: 1. Cost-effective preservation and cold-chain-free distribution of fully assembled cell-free reactions is possible and can facilitate the use of CFS in LMICs. https://t.co/kBxZWY0xt0

Jul 27, 2022 · 12 likes · 1,969 followers View post →
Fernando
@MrSorbicillin · X

@ATinyGreenCell @openbioeconomy https://t.co/scQE1nFzaw We managed to produce BsaI in cell-free, maybe can help you

Oct 7, 2024 · 11 likes · 2 shares · 195 followers View post →

All mentions

Nelke 'Demon Lord' Shilovich
@nelke_IGB · X

RT @MrSorbicillin: @ATinyGreenCell @openbioeconomy https://t.co/scQE1nFzaw We managed to produce BsaI in cell-free, maybe can help you

Oct 8, 2024 · 2 shares · 1,117 followers View post →
Sebastian S. Cocioba
@ATinyGreenCell · X

RT @MrSorbicillin: @ATinyGreenCell @openbioeconomy https://t.co/scQE1nFzaw We managed to produce BsaI in cell-free, maybe can help you

Oct 7, 2024 · 2 shares · 31,310 followers View post →
Fernando
@MrSorbicillin · X

@ATinyGreenCell @openbioeconomy https://t.co/scQE1nFzaw We managed to produce BsaI in cell-free, maybe can help you

Oct 7, 2024 · 11 likes · 2 shares · 195 followers View post →
雯雯❤郴州-(兼职|学生|约炮|上门)北湖区,苏仙区,资兴市,桂阳县,宜章县,永兴县,嘉禾县,临武县
@kittyclouston · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 3, 2023 · 16 shares · 488 followers View post →
MADscientist
@madscientist190 · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 3, 2023 · 16 shares · 147 followers View post →
Thev JayMahan
@ThevJayMahan · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 3, 2023 · 16 shares · 373 followers View post →
王思翀|SiChong Wang
@SichongW · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 3, 2023 · 16 shares · 154 followers View post →
Kenneth Timmis
@KennethTimmis · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 3, 2023 · 16 shares · 1,234 followers View post →
Nawaporn Onkokesung
@onkokesung · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 3, 2023 · 16 shares · 67 followers View post →
Tamjeed Saleh
@tamjeeds · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 206 followers View post →
Antonio J Vallecillo, PhD
@Vallecillo_AJ · X

Constructing cell-free expression systems for low-cost access. https://t.co/NOMhGZCR06

Oct 2, 2023 · 530 followers View post →
Animalcules
@bacteriophile · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 524 followers View post →
Roque Rivas
@rgrivasp71 · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 161 followers View post →
Sophien Kamoun
@KamounLab · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 39,979 followers View post →
J. Fernando Bazan @jfernandobazan.bsky.social
@jfernandobazan · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 702 followers View post →
Harmit S. Malik
@HarmitMalik · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 43,627 followers View post →
Gabriel Ortega
@Gabodabo · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 306 followers View post →
Diego de la Fuente
@Diego_official2 · X

RT @ATinyGreenCell: How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 16 shares · 634 followers View post →
Sebastian S. Cocioba
@ATinyGreenCell · X

How did I miss this paper from Jim's lab?! Gotta try this soon. https://t.co/d0tRcb54UQ

Oct 2, 2023 · 113 likes · 16 shares · 31,310 followers View post →
Open Bioeconomy Lab
@openbioeconomy · X

Critical points from our talk with Dr Fernando Guzman on Constructing Cell-Free Expression Systems for Low-Cost Access: 1. Cost-effective preservation and cold-chain-free distribution of fully assembled cell-free reactions is possible and can facilitate the use of CFS in LMICs. https://t.co/kBxZWY0xt0

Jul 27, 2022 · 12 likes · 1,969 followers View post →
Latif Adams, Ph.D, MSc, BSc
@latifadams2017 · X

RT @ShaloMinette: Want to learn more about constructing low-cost cell-free expression systems? Join us tomorrow (in person & online), 12:0…

Jul 26, 2022 · 2 shares · 478 followers View post →
Harry Akligoh
@harryakligoh · X

RT @ShaloMinette: Want to learn more about constructing low-cost cell-free expression systems? Join us tomorrow (in person & online), 12:0…

Jul 26, 2022 · 2 shares · 1,734 followers View post →
Vicky Reid
@VicsReid · X

RT @openbioeconomy: Still time to join us for a captivating talk by Dr Fernando Guzman-Chavez on Constructing Cell-Free Expression Systems…

Jul 26, 2022 · 2 shares · 154 followers View post →
Jenny Molloy
@jenny_molloy · X

RT @openbioeconomy: Still time to join us for a captivating talk by Dr Fernando Guzman-Chavez on Constructing Cell-Free Expression Systems…

Jul 26, 2022 · 2 shares · 3,753 followers View post →
Open Bioeconomy Lab
@openbioeconomy · X

Still time to join us for a captivating talk by Dr Fernando Guzman-Chavez on Constructing Cell-Free Expression Systems for Low-Cost Access. Wed 27th July, 12:00-13:00 BST In-person: @ LT3, CEB Online: Zoom RSVP https://t.co/jYydfMG79y #synbio #biotech #biomanufacturing https://t.co/DvbnMs9S7L

Jul 26, 2022 · 2 likes · 2 shares · 1,969 followers View post →
shalo minette
@ShaloMinette · X

Want to learn more about constructing low-cost cell-free expression systems? Join us tomorrow (in person & online), 12:00-13:00 BST for a talk on Constructing Cell-Free Expression Systems for Low-Cost Access with Dr Fernando Guzman-Chavez. #synbio #biotech #biomanufacturing https://t.co/vPE0TS7JK8

Jul 26, 2022 · 2 likes · 2 shares · 148 followers View post →
Fernando
@MrSorbicillin · X

RT @openbioeconomy: Upcoming: An exciting talk on Constructing Cell-Free Expression Systems for Low-Cost Access with Dr Fernando Guzman-Cha…

Jul 25, 2022 · 4 shares · 195 followers View post →
雯雯❤郴州-(兼职|学生|约炮|上门)北湖区,苏仙区,资兴市,桂阳县,宜章县,永兴县,嘉禾县,临武县
@kittyclouston · X

RT @openbioeconomy: Upcoming: An exciting talk on Constructing Cell-Free Expression Systems for Low-Cost Access with Dr Fernando Guzman-Cha…

Jul 25, 2022 · 4 shares · 488 followers View post →
Tata Gloria Berinyuy
@Berinyuy_7 · X

RT @openbioeconomy: Upcoming: An exciting talk on Constructing Cell-Free Expression Systems for Low-Cost Access with Dr Fernando Guzman-Cha…

Jul 25, 2022 · 4 shares · 12 followers View post →
Aishwarya Venkataramani
@Aishvenkatr · X

RT @openbioeconomy: Upcoming: An exciting talk on Constructing Cell-Free Expression Systems for Low-Cost Access with Dr Fernando Guzman-Cha…

Jul 25, 2022 · 4 shares · 368 followers View post →
Open Bioeconomy Lab
@openbioeconomy · X

Upcoming: An exciting talk on Constructing Cell-Free Expression Systems for Low-Cost Access with Dr Fernando Guzman-Chavez and Open Bioeconomy Lab. Join us on Wed 27 July, 12:00-13:00 @ LT3, CEB & online via Zoom RSVP https://t.co/jYydfMG79y #synbio #biotech #biomanufacturing https://t.co/o3eVQG3Gp7

Jul 25, 2022 · 15 likes · 4 shares · 1,969 followers View post →
Open Bioeconomy Lab
@openbioeconomy · X

Join the Open Bioeconomy Lab for a talk on Constructing Cell-Free Expression Systems for Low-Cost Access by Dr Fernando Guzman-Chavez. When: Wed 27 July, 12:00-13:00 BST Where: LT3, @cebcambridge & Online via Zoom RSVP https://t.co/jYydfMG79y #synbio #biotech #biomanufacturing

Jul 25, 2022 · 1 like · 1,969 followers View post →
Sebastian Maerkl
@SebastianMaerkl · X

Constructing Cell-Free Expression Systems for Low-Cost Access https://t.co/WBKTxycnvT

Mar 21, 2022 · 1 like · 868 followers View post →
dflackner
@drlackner · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 20, 2022 · 35 shares · 18 followers View post →
Invictus Samael
@InvictusSamael · X

RT @DrLeoRios: Low cost cell free systems #synbio

Mar 17, 2022 · 4 shares · 659 followers View post →
Tom Knight -- e/
@TomKnightSynBio · X

RT @DrLeoRios: Low cost cell free systems #synbio

Mar 17, 2022 · 4 shares · 2,937 followers View post →
Sebastian S. Cocioba
@ATinyGreenCell · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 17, 2022 · 35 shares · 31,310 followers View post →
ACS Synthetic Bio
@ACSSynBio · X

RT @plantsci: Our Synthetic Biology for Engineering Plant Growth Group has a new paper out in @ACSSynBio

Mar 17, 2022 · 2 shares · 11,080 followers View post →
ACS Synthetic Bio
@ACSSynBio · X

RT @DrLeoRios: Low cost cell free systems #synbio

Mar 17, 2022 · 4 shares · 11,080 followers View post →
Meow-Ludo
@meowludo · X

Constructing Cell-Free Expression Systems for Low-Cost Access https://t.co/3UvKlVx1P4

Mar 11, 2022 · 2 likes · 1,058 followers View post →
Leonardo Rios
@DrLeoRios · X

Low cost cell free systems #synbio

Mar 11, 2022 · 14 likes · 4 shares · 9,352 followers View post →
Sainsbury Laboratory Cambridge University (SLCU)
@slcuplants · X

RT @plantsci: Our Synthetic Biology for Engineering Plant Growth Group has a new paper out in @ACSSynBio

Mar 11, 2022 · 2 shares · 10,698 followers View post →
Carolina Rodríguez
@CarolUnam10 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 10, 2022 · 35 shares · 58 followers View post →
ArmKlamrak
@AKlamrak · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 10, 2022 · 35 shares · 39 followers View post →
FannyD
@LaTiteFanny35 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 10, 2022 · 35 shares · 83 followers View post →
Simon Moore
@sjmoore505 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 10, 2022 · 35 shares · 1,120 followers View post →
Department of Plant Sciences Cambridge
@camplantsci · X

Our Synthetic Biology for Engineering Plant Growth Group has a new paper out in @ACSSynBio

Mar 10, 2022 · 7 likes · 2 shares · 23,774 followers View post →
Giglione Carmela
@Giglionelab · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 10, 2022 · 35 shares · 288 followers View post →
Roberto BahenaC
@rabc92_ · X

https://t.co/yoZ9gMaCmN

Mar 10, 2022 · 1 like · 290 followers View post →
Invictus Samael
@InvictusSamael · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 659 followers View post →
Alaa Selim
@Synbio101 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 705 followers View post →
Toyota_Group_Komaba
@KomaToug · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 668 followers View post →
BiolocoUSB
@biolocousb · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 1,085 followers View post →
Guillermo Yáñez Feliú
@gyanezfeliu · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 304 followers View post →
Fermin
@LabTecLibre · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 339 followers View post →
Ezequiel Petrillo
@petry594 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 1,303 followers View post →
Peter Voyvodic
@PVoyvodic · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 426 followers View post →
Richard Kelwick
@rkelwick · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 1,345 followers View post →
Jim Haseloff
@jimhaseloff · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 5,172 followers View post →
Khalid K. Alam ‎خالد كمال عالم
@BiochemPhD · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 1,558 followers View post →
The Lucks Lab
@LucksLab · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 3,235 followers View post →
Diego de la Fuente
@Diego_official2 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 634 followers View post →
Dr. Larry, PhD-MBA
@larrythewarrior · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 517 followers View post →
Leonel Magnaterra
@MagnaLeonel · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 330 followers View post →
synbiopapers
@synbio_papers · X

Constructing Cell-Free Expression Systems for Low-Cost Access: Cell-free systems for gene expression have gained attention as platforms for the facile study of genetic circuits and as highly effective tools for teaching. Despite recent progress, the… https://t.co/a4aIpmIa1d https://t.co/Er0utHblwF

Mar 9, 2022 · 4 likes · 4,047 followers View post →
Philippe Mozzanega
@PMozzanega · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 464 followers View post →
JosePedroza
@JosePed32082343 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 34 followers View post →
Eric Velasco Yépez
@EricAndreVY · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 1,112 followers View post →
David Rubert, PhD
@David__Rubert · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 641 followers View post →
WeiTing Lin(GR15
@weitinglin66 · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 1,290 followers View post →
Olivier Borkowski
@O_Borkowski · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 7,106 followers View post →
Anna Sze Wai Tse
@Marchanti_anna · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 106 followers View post →
Facundo Romani
@facundoromani · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 1,365 followers View post →
Filippo Caschera
@Stjle · X

RT @MrSorbicillin: Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without…

Mar 9, 2022 · 35 shares · 490 followers View post →
Fernando
@MrSorbicillin · X

Our paper is alive!!!. Do you know that you can dry cell-free reaction using a desiccator? or even perform CFPS without adding any exogenous NTPs?... These and more information here https://t.co/0EkpomkMAt

Mar 8, 2022 · 110 likes · 35 shares · 195 followers View post →
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