nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on the microscope glass slides. Research article: https://t.co/NanDyKq8Iu Support website: https://t.co/LbH5B741Op Protocol: https://t.co/3BUcQymGKG https://t.co/SG2kZ3wRfp
Exploring functional protein covariation across single cells using nPOP.
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Apr 2022 – Sep 2025Where it's discussed
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X / Twitter86 posts
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YouTube8 videos
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Blogs3 articles
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Facebook2 posts
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News2 articles
AI synopsis
What people are saying
The overall discourse demonstrates a strong positive stance, with several posts emphasizing the innovative aspects of nPOP, such as its flexibility and automation, as 'game-changing' or 'supporting pipeline development.' The tone conveys excitement about the research's potential to advance biological understanding, reflected in phrases like 'fundamental research' and 'high throughput processing,' illustrating both interest and perceived significance.
Many discussions express strong support for the research’s significance and methods, indicating broad agreement on its importance.
Posts show high curiosity, with mentions of novel methods and enthusiasm about the research findings.
The discourse is detailed, referencing specific techniques like protein covariation analysis, and sharing insights on experimental strategies.
Users highlight the potential transformative effects on single-cell proteomics and related fields, underscoring its high scientific impact.
Abstract in brief
- nPOP is a groundbreaking method for preparing thousands of single cells for proteomic analysis by using piezo acoustic dispensing and performing sample preparation in tiny droplets, allowing for high-throughput processing.
- The analysis of protein covariation reveals significant differences in cell cycle dynamics and energy utilization between different populations of melanoma cells, highlighting how some cells resistant to drugs accumulate in the G1 phase and have altered protein expressions.
- This advancement in single-cell proteomics through nPOP facilitates a deeper understanding of biological processes and supports the investigation of functional differences among closely related cell states, enhancing research in drug resistance and cell behavior.
Top posts
Most liked on X and BlueskyIs protein covariation across single cells from a cluster biologically informative? We investigated this question in our new @GenomeBiology article led by @_AndrewLeduc https://t.co/NanDyKq8Iu Highlights that stand out for me include
Excited our paper is finally out!
A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co/DZ0E7vCEbp). Single-Cell Proteomics Bypasses Bottlenecks, Sways Skeptics https://t.co/42r2BxkfZO
nPOP, from @_AndrewLeduc, @slavovLab & co, is a flexible, automated, and parallelized pipeline for single-cell proteomics sample preparation, allowing protein covariation studies in thousands of single cells. Allows identification of melanoma subtypes. https://t.co/FTNWq4Dn9L https://t.co/9JSQjDitS7
This reminds me of our observation of cell-type specific protein covariation https://t.co/NanDyKqGy2 https://t.co/hZw3GEJSZt
All mentions
single-cell proteomics sample preparation with nPOP method
This video demonstrates how nPOP pools droplets containing proteome samples from single cells and transfers them to a multiwell plate, facilitating automated LCMSMS analysis for high-throughput proteomics research.
Advances in Single-Cell Proteomics Using Mass Spectrometry Techniques
This lecture introduces single-cell proteomics by mass spectrometry, highlighting plexDIA's high-throughput multiplexing capabilities for small samples, enabling detailed protein analysis in individual human cells.
Single-cell Mass Spectrometry Proteomics Data Acquisition and Analysis Techniques
Workshop on single-cell mass spectrometry proteomic data acquisition using plexDIA and timsTOF, with initial interpretation via QuantQC, held at SCP2024. Focuses on improving data quality by minimizing protein leakage…
Advancements in Single-Cell Proteomics Techniques
This presentation by Prof. Nikolai Slavov at SCP2023 covers advancements in single-cell proteomics, highlighting how new techniques can analyze variations in protein interactions across individual cells. Understanding…
Advancements in Single-Cell Proteomics with plexDIA Technology
Exploring new methodologies for sensitive protein analysis in single cells, focusing on plexDIA's framework that enhances throughput, accuracy, and proteome coverage for low sample amounts.
Parallel Sample Preparation for Single-Cell Proteomics Using nPOP
This workshop covers highly parallel sample preparation for multiplexed single-cell proteomics using nPOP by Dr. Josh Cantlon at SCP2024. It introduces CellenOne for isolating single-cell samples for mass spectrometry…
Enhancing Single-Cell Proteomics: Techniques and Data Interpretation
Parallel Sample Preparation for Single-Cell Proteomics Using nPOP
This workshop covers highly parallel sample prep for multiplexed single-cell proteomics with nPOP by Dr. Josh Cantlon. It introduces CellenOne for isolating single cells for LCMS analysis, demonstrating processing over…
RT @_AndrewLeduc: My first project was designing a new sample preparation method to generate high quality single cell proteomics samples. O…
My first project was designing a new sample preparation method to generate high quality single cell proteomics samples. Our design was flexible and allowed us to adapt to any multiplexing strategy with easily programable droplet positions https://t.co/q2Pq8vXwz9 https://t.co/SzorlWzLhD
RT @slavovLab: Next week, @shiri_tsour and @_AndrewLeduc will be defending their dissertations ! They will present research from: https:…
Next week, @shiri_tsour and @_AndrewLeduc will be defending their dissertations ! They will present research from: https://t.co/dObDKJ85EJ https://t.co/gyIhKSKRi8 https://t.co/hpnd38VDE1 https://t.co/7nqZfGAsZ7 and more
RT @ParallelSqTech: We hosted the @SCP_meeting workshop, and a recording is up on YouTube: Highly parallel sample preparation for multiple…
RT @ParallelSqTech: We hosted the @SCP_meeting workshop, and a recording is up on YouTube: Highly parallel sample preparation for multiple…
We hosted the @SCP_meeting workshop, and a recording is up on YouTube: Highly parallel sample preparation for multiplexed single-cell proteomics using nPOP Workshop @ #SCP2024 https://t.co/oTIgLAtyM7
@ProteomicsNews @AmandaLSmythers I think the npop methods should be cheaper? Not listed at present - e.g. https://t.co/8WCRF0cUa0 and https://t.co/H6tA0D8okv.
RT @slavov_n: It’s good to see data from @slavovLab (https://t.co/NanDyKq8Iu) used for further analysis and pipeline development: https://t…
RT @slavov_n: It’s good to see data from @slavovLab (https://t.co/NanDyKq8Iu) used for further analysis and pipeline development: https://t…
RT @slavov_n: It’s good to see data from @slavovLab (https://t.co/NanDyKq8Iu) used for further analysis and pipeline development: https://t…
It’s good to see data from @slavovLab (https://t.co/NanDyKq8Iu) used for further analysis and pipeline development: https://t.co/tILtNdHjsq Data sharing and reanalysis are the foundations of healthy & robust research. https://t.co/zgZWMblEzu
@Nick_Schaum We have to understand how random accumulation of damage in each cell then manifest as aging. I would propose… scPROT https://t.co/SHRyGjxac8 scATAC long reads https://t.co/vQBtSdfWvy scWGS + scATAC https://t.co/l6oHaEGtzP scMET https://t.co/H7PR6smGtp
This path requires a flexible sample prep that can be adapted to any multiplexing scheme. That is exactly what nPOP enables: - One can digitally program any desired plex and prepare thousands of single cells at once. https://t.co/NanDyKq8Iu
RT @_AndrewLeduc: @SchejbalJLab Yeah for things like histones you dont want to normalize them to cell size, its a bit more complex. Ideally…
@SchejbalJLab Yeah for things like histones you dont want to normalize them to cell size, its a bit more complex. Ideally if you have cell size like you do in https://t.co/q2Pq8vXwz9, you can compute separate capture efficiency and size estimates and normalize histones only for capt eff
@DemichevLab This statement is well supported by the data, e.g., the negative controls in panel c. https://t.co/NanDyKq8Iu https://t.co/sQrGfoJe6W
@SchejbalJLab @SCP_meeting We will aim for a general introduction and demonstrations useful to different approaches. We will also aim to demonstrate nPOP https://t.co/NanDyKq8Iu
@lkpino @wfondrie In single cell analysis you get the diameter of each cell as well ;) https://t.co/m2ab1hpKwE
@JMarchingo @NorSivaeb @AJ_Brenes @ProteomicsNews We can evaluate these relationships quite nicely with single cell analysis: https://t.co/m2ab1hpKwE
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
RT @slavov_n: A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co…
A new @GENbio article highlights research from @slavovLab, including nPOP (https://t.co/NanDyKqGy2) and plexDIA (https://t.co/DZ0E7vCEbp). Single-Cell Proteomics Bypasses Bottlenecks, Sways Skeptics https://t.co/42r2BxkfZO
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
RT @slavov_n: nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on…
nPOP in action, preparing cells for single-cell proteomics. Each cell is processed in a droplet. Thousands of droplets fit on the microscope glass slides. Research article: https://t.co/NanDyKq8Iu Support website: https://t.co/LbH5B741Op Protocol: https://t.co/3BUcQymGKG https://t.co/SG2kZ3wRfp
RT @slavov_n: This reminds me of our observation of cell-type specific protein covariation https://t.co/NanDyKqGy2 https://t.co/hZw3GEJSZt
RT @slavov_n: This reminds me of our observation of cell-type specific protein covariation https://t.co/NanDyKqGy2 https://t.co/hZw3GEJSZt
This reminds me of our observation of cell-type specific protein covariation https://t.co/NanDyKqGy2 https://t.co/hZw3GEJSZt
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @GenomeBiology: nPOP, from @_AndrewLeduc, @slavovLab & co, is a flexible, automated, and parallelized pipeline for single-cell proteomic…
RT @GenomeBiology: nPOP, from @_AndrewLeduc, @slavovLab & co, is a flexible, automated, and parallelized pipeline for single-cell proteomic…
nPOP, from @_AndrewLeduc, @slavovLab & co, is a flexible, automated, and parallelized pipeline for single-cell proteomics sample preparation, allowing protein covariation studies in thousands of single cells. Allows identification of melanoma subtypes. https://t.co/FTNWq4Dn9L https://t.co/9JSQjDitS7
The research from this #SCP2021 presentation was published in @GenomeBiology: https://t.co/CrpsDjpS7p https://t.co/Srf8Qs9qfD https://t.co/FIMtLnOpe8
RT @PastelBio: Exploring functional protein covariation across single cells using nPOP | https://t.co/Jc3bLihwIf #proteomics https://t.co/D…
Exploring functional protein covariation across single cells using nPOP | https://t.co/Jc3bLihwIf #proteomics https://t.co/DVUZdxiqLW
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @_AndrewLeduc: Excited our paper is finally out!
RT @_AndrewLeduc: Excited our paper is finally out!
RT @_AndrewLeduc: Excited our paper is finally out!
RT @_AndrewLeduc: Excited our paper is finally out!
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @_AndrewLeduc: Excited our paper is finally out!
RT @_AndrewLeduc: Excited our paper is finally out!
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @_AndrewLeduc: Excited our paper is finally out!
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @_AndrewLeduc: Excited our paper is finally out!
Exploring functional protein covariation across single cells using nPOP https://t.co/jpjWXcZ3wr
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @_AndrewLeduc: Excited our paper is finally out!
RT @_AndrewLeduc: Excited our paper is finally out!
RT @_AndrewLeduc: Excited our paper is finally out!
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
Excited our paper is finally out!
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
RT @slavov_n: Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new…
Is protein covariation across single cells from a cluster biologically informative? We investigated this question in our new @GenomeBiology article led by @_AndrewLeduc https://t.co/NanDyKq8Iu Highlights that stand out for me include
RT @PastelBio: Exploring functional protein covariation across single cells using nPOP | https://t.co/jMvKwhk1YO #proteomics @slavovLab htt…
RT @PastelBio: Exploring functional protein covariation across single cells using nPOP | https://t.co/jMvKwhk1YO #proteomics @slavovLab htt…
Exploring functional protein covariation across single cells using nPOP | https://t.co/jMvKwhk1YO #proteomics @slavovLab https://t.co/kUGhLex2Xs