Genome Med May 2023 PMID 37127706

Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles.

Randy B Kang, Yansui Li, Carolina Rosselot, Tuo Zhang, Mustafa Siddiq, Prashant Rajbhandari, Andrew F Stewart, Donald K Scott, Adolfo Garcia-Ocana, Geming Lu

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Mentions

32
Peak: 31 in May 2023
across 2 sources since May 2023

Extended reach

27,203
potential audience

Total likes

86

Video views

20

Mentions per month

May 2023 – Sep 2023

Where it's discussed

  • X / Twitter31 posts
  • YouTube1 videos

AI synopsis

What people are saying

Discussions frequently emphasize the innovative use of snRNA-seq and pre-mRNA analysis to distinguish β-cell subpopulations and identify novel gene markers, with phrases like 'accurately identify', 'dynamic transcriptome profiles', and 'new marker finding' underscoring the perceived importance. The tone remains highly positive, reflecting excitement about the potential implications for diabetes research and cell biology.

AgreementStrong agreement

Many discussions express strong support and enthusiasm for the research, viewing it as a significant advancement in understanding pancreatic islet cells, as seen in praise for the identification of novel gene sets and subpopulations.

InterestHigh interest

Posts consistently show high curiosity and relevance, with multiple comments highlighting the importance of techniques like snRNA-seq and pre-mRNA analysis for diabetes research, indicating genuine engagement.

EngagementHigh engagement

Several discussions reference specific methods, such as RNA imaging and transcript profiling, and mention the paper’s implications for understanding β-cell heterogeneity, demonstrating deep engagement with the scientific content.

ImpactHigh impact

The collective tone suggests a high perceived impact, with posters describing the study as 'a game changer' and emphasizing its potential to advance diabetes research, thus recognizing its high potential influence.

Abstract in brief

  • Single-nucleus RNA sequencing (snRNAseq) allows for the study of human pancreatic islet cells using frozen samples without the need for cell dissociation, highlighting its advantages over single-cell RNA sequencing (scRNAseq) which requires cell dissociation and is less suitable for in vivo studies.
  • The study identified novel noncanonical gene markers that are differentially expressed in human islet cells both in vitro and in vivo, expanding understanding beyond traditional gene markers and providing new insights into cell-specific gene expression profiles.
  • By integrating snRNAseq with scRNAseq data, researchers distinguished three distinct subpopulations of insulin-producing (INS) cells, each with unique gene expression patterns, and observed that the INS mRNArich subcluster becomes dominant in vivo, indicating dynamic transcriptome profiles and biological states.

Top posts

Most liked on X and Bluesky
Geming (Brian) Lu
@Brian74534732 · X

Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpopulations, and their dynamic transcriptome profile in vivo. @GenomeMedicine @AGOcanaLab_COH #diabetes #betacell https://t.co/LMu1VCyEIS https://t.co/T96KFJYlyJ

May 4, 2023 · 35 likes · 11 shares · 728 followers View post →
Adolfo Garcia Ocaña. Ph.D.
@AGOcanaLab_COH · X

Shout #Diabetesresearch folks. Our paper is online @GenomeMedicine @SpringerNature. Single nucleus RNA sequencing of human pancreatic #islet identifies novel gene sets and distinguishes #βcell subpopulations with dynamic transcriptome profiles. https://t.co/2MUq7FLWIj @cityofhope https://t.co/MDoNSvyxiB

May 4, 2023 · 25 likes · 9 shares · 157 followers View post →
Randy Kang
@randystyle · X

Novel gene sets / distinguishes β-cell subpopulations @GenomeMedicine paper @SpringerNature for new marker finding and #betacell heterogeniety @AGOcanaLab_COH lab @cityofhope, previously at @mountsinai. Read at https://t.co/SHyAV9hxc3 #pancreas #diabetes #scRNAseq #islets https://t.co/u2UdiIa1yb

May 3, 2023 · 10 likes · 8 shares · 73 followers View post →
Randy Kang
@randystyle · X

Finally, my first paper is published and online!!! @GenomeMedicine https://t.co/NSHFE6X59D

May 1, 2023 · 9 likes · 3 shares · 73 followers View post →
bindu-sundaresan
@ScSpatial_Splst · X

Congratulations @Brian74534732, @AGOcanaLab_COH and team! Nice to see RNAscope images to distinguish between pre-mRNA and mRNA, combined with insulin IF for beta cell marker. https://t.co/u86eG2z39q https://t.co/FpIPsq56am

May 5, 2023 · 4 likes · 2 shares · 353 followers View post →
Single Cell papers
@scell_papers · X

Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles https://t.co/lG5KSmIbTs

May 4, 2023 · 1 like · 11,512 followers View post →

All mentions

Adolfo Garcia Ocaña. Ph.D.
@AGOcanaLab_COH · X

RT @spatialRNA_ISH: Congratulations @Brian74534732, @AGOcanaLab_COH and team! Nice to see RNAscope images to distinguish between pre-mRNA a…

May 5, 2023 · 2 shares · 157 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

RT @isletbiologylab: Kang et al. propose that snRNA-seq and pre-mRNA analysis can accurately identify human #islet cell populations, subpop…

May 5, 2023 · 1 share · 728 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

RT @spatialRNA_ISH: Congratulations @Brian74534732, @AGOcanaLab_COH and team! Nice to see RNAscope images to distinguish between pre-mRNA a…

May 5, 2023 · 2 shares · 728 followers View post →
bindu-sundaresan
@ScSpatial_Splst · X

Congratulations @Brian74534732, @AGOcanaLab_COH and team! Nice to see RNAscope images to distinguish between pre-mRNA and mRNA, combined with insulin IF for beta cell marker. https://t.co/u86eG2z39q https://t.co/FpIPsq56am

May 5, 2023 · 4 likes · 2 shares · 353 followers View post →
Islet Biology Lab - UMH
@isletbiologylab · X

Kang et al. propose that snRNA-seq and pre-mRNA analysis can accurately identify human #islet cell populations, subpopulations, and their dynamic #transcriptome profile in vitro and in vivo in human islet grafts. https://t.co/TQZ3Uc736s

May 5, 2023 · 1 like · 1 share · 1,151 followers View post →
Ana Viñuela
@anavinuela · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 1,375 followers View post →
Single Cell papers
@scell_papers · X

Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles https://t.co/lG5KSmIbTs

May 4, 2023 · 1 like · 11,512 followers View post →
Adolfo Garcia Ocaña. Ph.D.
@AGOcanaLab_COH · X

RT @AGOcanaLab_COH: Shout #Diabetesresearch folks. Our paper is online @GenomeMedicine @SpringerNature. Single nucleus RNA sequencing of hu…

May 4, 2023 · 9 shares · 157 followers View post →
Randy Kang
@randystyle · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 73 followers View post →
Randy Kang
@randystyle · X

RT @AGOcanaLab_COH: Shout #Diabetesresearch folks. Our paper is online @GenomeMedicine @SpringerNature. Single nucleus RNA sequencing of hu…

May 4, 2023 · 9 shares · 73 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

RT @AGOcanaLab_COH: Shout #Diabetesresearch folks. Our paper is online @GenomeMedicine @SpringerNature. Single nucleus RNA sequencing of hu…

May 4, 2023 · 9 shares · 728 followers View post →
Adolfo Garcia Ocaña. Ph.D.
@AGOcanaLab_COH · X

Shout #Diabetesresearch folks. Our paper is online @GenomeMedicine @SpringerNature. Single nucleus RNA sequencing of human pancreatic #islet identifies novel gene sets and distinguishes #βcell subpopulations with dynamic transcriptome profiles. https://t.co/2MUq7FLWIj @cityofhope https://t.co/MDoNSvyxiB

May 4, 2023 · 25 likes · 9 shares · 157 followers View post →
Brian Lu
@BrianLu384884 · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 1 followers View post →
IBE
@DrDx11696608 · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 94 followers View post →
Jiarui Mi
@JiaruiMi · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 985 followers View post →
Jason Bini, PhD
@ScienceDrBini · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 1,021 followers View post →
Adolfo Garcia Ocaña. Ph.D.
@AGOcanaLab_COH · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 157 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

RT @Brian74534732: Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpop…

May 4, 2023 · 11 shares · 728 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

Using #snRNAseq and pre-mRNA analysis of human #islet cells can accurately identify human islet cell populations, subpopulations, and their dynamic transcriptome profile in vivo. @GenomeMedicine @AGOcanaLab_COH #diabetes #betacell https://t.co/LMu1VCyEIS https://t.co/T96KFJYlyJ

May 4, 2023 · 35 likes · 11 shares · 728 followers View post →
Yunkyeong Lee
@yunkyeong_lee · X

RT @randystyle: Novel gene sets / distinguishes β-cell subpopulations @GenomeMedicine paper @SpringerNature for new marker finding and #bet…

May 3, 2023 · 8 shares · 113 followers View post →
beckie boyun choi
@missy_Becks · X

RT @randystyle: Novel gene sets / distinguishes β-cell subpopulations @GenomeMedicine paper @SpringerNature for new marker finding and #bet…

May 3, 2023 · 8 shares · 7 followers View post →
Adolfo Garcia Ocaña. Ph.D.
@AGOcanaLab_COH · X

RT @randystyle: Finally, my first paper is published and online!!! @GenomeMedicine https://t.co/NSHFE6X59D

May 3, 2023 · 3 shares · 157 followers View post →
Adolfo Garcia Ocaña. Ph.D.
@AGOcanaLab_COH · X

RT @randystyle: Novel gene sets / distinguishes β-cell subpopulations @GenomeMedicine paper @SpringerNature for new marker finding and #bet…

May 3, 2023 · 8 shares · 157 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

RT @randystyle: Novel gene sets / distinguishes β-cell subpopulations @GenomeMedicine paper @SpringerNature for new marker finding and #bet…

May 3, 2023 · 8 shares · 728 followers View post →
Randy Kang
@randystyle · X

Novel gene sets / distinguishes β-cell subpopulations @GenomeMedicine paper @SpringerNature for new marker finding and #betacell heterogeniety @AGOcanaLab_COH lab @cityofhope, previously at @mountsinai. Read at https://t.co/SHyAV9hxc3 #pancreas #diabetes #scRNAseq #islets https://t.co/u2UdiIa1yb

May 3, 2023 · 10 likes · 8 shares · 73 followers View post →
Transcriptomes
@transcriptomes · X

Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and disti... https://t.co/wqIHJHKuRo

May 2, 2023 · 1,808 followers View post →
Clustering ::.
@clusteringpaper · X

Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles https://t.co/wFk3RORkyA

May 2, 2023 · 20 followers View post →
CompBiolPapers
@CompBiolPapers · X

Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles https://t.co/WP7m8up7mi

May 2, 2023 · 1 like · 1,780 followers View post →
Geming (Brian) Lu
@Brian74534732 · X

RT @randystyle: Finally, my first paper is published and online!!! @GenomeMedicine https://t.co/NSHFE6X59D

May 2, 2023 · 3 shares · 728 followers View post →
Jane Velghe
@Kc2Canada · X

RT @randystyle: Finally, my first paper is published and online!!! @GenomeMedicine https://t.co/NSHFE6X59D

May 1, 2023 · 3 shares · 244 followers View post →
Randy Kang
@randystyle · X

Finally, my first paper is published and online!!! @GenomeMedicine https://t.co/NSHFE6X59D

May 1, 2023 · 9 likes · 3 shares · 73 followers View post →