PLoS Biol June 2023 PMID 37310918

Development of an amplicon-based sequencing approach in response to the global emergence of mpox.

Nicholas F G Chen, Chrispin Chaguza, Luc Gagne, Matthew Doucette, Sandra Smole, Erika Buzby, Joshua Hall, Stephanie Ash, Rachel Harrington, Seana Cofsky, Selina Clancy, Curtis J Kapsak, Joel Sevinsky, Kevin Libuit, Daniel J Park

Mentions

39
Peak: 26 in Jun 2023
across 3 sources since Jan 2023

Extended reach

767,496
potential audience

Total likes

54

Video views

24

Mentions per month

Jan 2023 – Aug 2024

Where it's discussed

  • X / Twitter32 posts
  • News6 articles
  • YouTube1 videos

AI synopsis

What people are saying

Several discussions underscore the importance of the publication by describing it as a 'timely' solution to mpox's rise, with words like 'response' and 'development' conveying urgency and utility. The tone varies from technical appreciation—highlighting aspects like 'primer schemes' and 'bioinformatics infrastructure'—to acknowledgment of its potential impact on public health efforts, demonstrating a mix of technical engagement and recognition of its significance.

AgreementModerate agreement

Most discussions endorse the publication, highlighting its timely relevance and potential utility, with phrases like 'timely given' and descriptions of the method as a 'response' to mpox's emergence.

InterestHigh interest

The majority of posts express strong curiosity and relevance, showing excitement about the new sequencing approach and its application for mpox detection.

EngagementHigh engagement

Many discussions delve into methodological details, such as mentioning 'amplicon-based sequencing,' 'primer schemes,' and infrastructure compatibility, indicating active analysis and appreciation for technical aspects.

ImpactModerate impact

Posts suggest the publication could significantly impact future genomic surveillance and public health responses, though some are cautious and emphasize ongoing need for monitoring and validation.

Abstract in brief

  • The 2022 mpox outbreak during the COVID-19 pandemic emphasized the need for better genomic surveillance and whole-genome sequencing due to limitations in current metagenomic sequencing methods.
  • To address these limitations, researchers adapted an existing sequencing approach (PrimalSeq), which was previously used for Zika and SARS-CoV-2, to effectively sequence the human monkeypox virus.
  • Findings indicated that using amplicon-based sequencing resulted in higher genome coverage, especially for samples with lower viral DNA levels, and recommendations were made to select samples with a PCR cycle threshold below 31 for optimal results.

Top posts

Most liked on X and Bluesky
PLOS Biology
@PLOSBiology · X

Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/ChvwaLUcxX

Jun 14, 2023 · 16 likes · 10 shares · 103,150 followers View post →
Nicholas Chen, MPH
@Nick_FG_Chen · X

Jun 14, 2023 · 12 likes · 4 shares · 64 followers View post →
PLOS Biology
@PLOSBiology · X

Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/0h4YKicqsM

Jun 14, 2023 · 6 likes · 1 share · 103,150 followers View post →
Nonia Pariente
@npariente · X

Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development of a sequencing approach in response to the global emergence of mpox https://t.co/wlJn479wGp

Jun 20, 2023 · 6 likes · 2 shares · 4,584 followers View post →
Roland Baker
@RolandBakerIII · X

Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus (H/T @outbreakupdates) https://t.co/CyyhJ4Vh3p

Jan 18, 2023 · 5 likes · 8,172 followers View post →
PLOS Biology
@PLOSBiology · X

WHO has declared another public health emergency given mpox spread in Africa. This paper describes an affordable amplicon-based sequencing approach in response to the global emergence of #mpox developed by @VogelsChantal and a large group of coauthors https://t.co/7lWQfOJ8DC

Aug 14, 2024 · 4 likes · 103,150 followers View post →

All mentions

PLOS Biology
@PLOSBiology · X

WHO has declared another public health emergency given mpox spread in Africa. This paper describes an affordable amplicon-based sequencing approach in response to the global emergence of #mpox developed by @VogelsChantal and a large group of coauthors https://t.co/7lWQfOJ8DC

Aug 14, 2024 · 4 likes · 103,150 followers View post →
Biological Sciences | University of Edinburgh
@SBSatEd · X

RT @edin_EID:

Jul 12, 2023 · 1 share · 3,125 followers View post →
Edinburgh Infectious Diseases
@edin_EID · X

Jul 12, 2023 · 1 share · 7,049 followers View post →
Eng Eong Ooi
@EngEongOoi · X

RT @npariente: Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development o…

Jun 20, 2023 · 2 shares · 1,806 followers View post →
PLOS Biology
@PLOSBiology · X

RT @npariente: Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development o…

Jun 20, 2023 · 2 shares · 103,150 followers View post →
Nonia Pariente
@npariente · X

Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development of a sequencing approach in response to the global emergence of mpox https://t.co/wlJn479wGp

Jun 20, 2023 · 6 likes · 2 shares · 4,584 followers View post →
One Health Genomics Edinburgh
@GenomicsEdi · X

New paper: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @RIE_Lothian https://t.co/NoWZ6wCyVh

Jun 20, 2023 · 1,159 followers View post →
Viral Metagenomics
@papers_viral · X

Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/F43MWQNVFQ https://t.co/fGAyeP1B2E

Jun 17, 2023 · 1 like · 164 followers View post →
Bloodsucking Parasites
@bloodSparasites · X

RT @bloodSparasites: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://…

Jun 15, 2023 · 2 shares · 5,088 followers View post →
PLOS Biology
@PLOSBiology · X

Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhb16 https://t.co/rZ9201wXjA

Jun 15, 2023 · 1 like · 103,150 followers View post →
Kevin G. Libuit
@kevinlibuit · X

RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…

Jun 14, 2023 · 10 shares · 474 followers View post →
Nicole Feriancek
@NicoleFeriancek · X

RT @Nick_FG_Chen:

Jun 14, 2023 · 4 shares · 31 followers View post →
russ, mph
@aesthetics_grad · X

RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…

Jun 14, 2023 · 10 shares · 951 followers View post →
Luc Gagne
@lagagne01 · X

RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…

Jun 14, 2023 · 1 share · 64 followers View post →
aimee
@ceniseros_a · X

RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…

Jun 14, 2023 · 10 shares · 59 followers View post →
aimee
@ceniseros_a · X

RT @Nick_FG_Chen:

Jun 14, 2023 · 4 shares · 59 followers View post →
PLOS Biology
@PLOSBiology · X

Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/0h4YKicqsM

Jun 14, 2023 · 6 likes · 1 share · 103,150 followers View post →
bostonjetcharter.com
@Gifted_udeh · X

RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…

Jun 14, 2023 · 10 shares · 131 followers View post →
Chrispin Chaguza
@ChrispinChaguza · X

RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…

Jun 14, 2023 · 10 shares · 1,579 followers View post →
russ, mph
@aesthetics_grad · X

RT @Nick_FG_Chen:

Jun 14, 2023 · 4 shares · 951 followers View post →
Nicholas Chen, MPH
@Nick_FG_Chen · X

RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…

Jun 14, 2023 · 10 shares · 64 followers View post →
PLOS Biology
@PLOSBiology · X

RT @Nick_FG_Chen:

Jun 14, 2023 · 4 shares · 103,150 followers View post →
Nicholas Chen, MPH
@Nick_FG_Chen · X

Jun 14, 2023 · 12 likes · 4 shares · 64 followers View post →
PLOS Biology
@PLOSBiology · X

Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/ChvwaLUcxX

Jun 14, 2023 · 16 likes · 10 shares · 103,150 followers View post →
PLOS Biology
@PLOSBiology · X

RT @bloodSparasites: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://…

Jun 14, 2023 · 2 shares · 103,150 followers View post →
Bloodsucking Parasites
@bloodSparasites · X

Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://t.co/k0Cc15HbAR https://t.co/EF77wpNVfW

Jun 14, 2023 · 2 likes · 2 shares · 5,088 followers View post →
micropapers
@micro_papers · X

Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/EK9Vk9F9RG

Jun 13, 2023 · 332 followers View post →
Phage Papers
@Phage_Papers · X

Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/AqYssL4aQ5

Jun 13, 2023 · 647 followers View post →
New England BioGroup
@nebiogroup · X

Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/Q7c5Fywfyt

Jun 13, 2023 · 169 followers View post →
Roland Baker
@RolandBakerIII · X

Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus (H/T @outbreakupdates) https://t.co/CyyhJ4Vh3p

Jan 18, 2023 · 5 likes · 8,172 followers View post →
Awakened Guardian
@Tian_A1 · X

Exploring a novel amplicon-based mpox virus sequencing strategy [ https://t.co/tNa2RmXayP ] Chen, Chrispin Chaguza, Luc Gagne, et al. (2023 ).Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox https://t.co/qzKC8NRgJH

Jan 18, 2023 · 2,024 followers View post →
Outbreak Science
@outbreaksci · X

New preprint: Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus https://t.co/46Y6a0Bqwm #Zika #monkeypox #COVID19

Jan 16, 2023 · 1,376 followers View post →