Development of an amplicon-based sequencing approach in response to the global emergence of mpox.

Nicholas F G Chen, Chrispin Chaguza, Luc Gagne, Matthew Doucette, Sandra Smole, Erika Buzby, Joshua Hall, Stephanie Ash, Rachel Harrington, Seana Cofsky, Selina Clancy, Curtis J Kapsak, Joel Sevinsky, Kevin Libuit, Daniel J Park

June 2023 PLoS Biol

Synopsis of Social media discussions

Several discussions underscore the importance of the publication by describing it as a 'timely' solution to mpox's rise, with words like 'response' and 'development' conveying urgency and utility. The tone varies from technical appreciation—highlighting aspects like 'primer schemes' and 'bioinformatics infrastructure'—to acknowledgment of its potential impact on public health efforts, demonstrating a mix of technical engagement and recognition of its significance.

A
Agreement
 Moderate agreement

Most discussions endorse the publication, highlighting its timely relevance and potential utility, with phrases like 'timely given' and descriptions of the method as a 'response' to mpox's emergence.

I
Interest
 High level of interest

The majority of posts express strong curiosity and relevance, showing excitement about the new sequencing approach and its application for mpox detection.

E
Engagement
 High engagement

Many discussions delve into methodological details, such as mentioning 'amplicon-based sequencing,' 'primer schemes,' and infrastructure compatibility, indicating active analysis and appreciation for technical aspects.

I
Impact
 Moderate level of impact

Posts suggest the publication could significantly impact future genomic surveillance and public health responses, though some are cautious and emphasize ongoing need for monitoring and validation.

Social Mentions

YouTube

1 Videos

Twitter

32 Posts

News

6 Articles

Metrics

Video Views

24

Total Likes

54

Extended Reach

767,496

Social Features

39

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Enhanced Amplicon Sequencing Method for Monkeypox Virus Detection

Enhanced Amplicon Sequencing Method for Monkeypox Virus Detection

The 2022 mpox outbreak highlighted the need for improved genomic surveillance. This video discusses how researchers adapted an existing sequencing approach to effectively analyze the human monkeypox virus, improving genome coverage especially in low viral DNA samples.

Social RTCL TV

September 15, 2023

24 views

  • PLOS Biology
    @PLOSBiology (Twitter)

    WHO has declared another public health emergency given mpox spread in Africa. This paper describes an affordable amplicon-based sequencing approach in response to the global emergence of #mpox developed by @VogelsChantal and a large group of coauthors https://t.co/7lWQfOJ8DC
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    August 14, 2024

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  • Biological Sciences | University of Edinburgh
    @SBSatEd (Twitter)

    RT @edin_EID:
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    July 12, 2023

    1

  • Edinburgh Infectious Diseases
    @edin_EID (Twitter)


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    July 12, 2023

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  • Eng Eong Ooi
    @EngEongOoi (Twitter)

    RT @npariente: Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development o…
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    June 20, 2023

    2

  • PLOS Biology
    @PLOSBiology (Twitter)

    RT @npariente: Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development o…
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    June 20, 2023

    2

  • Nonia Pariente
    @npariente (Twitter)

    Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development of a sequencing approach in response to the global emergence of mpox https://t.co/wlJn479wGp
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    June 20, 2023

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  • One Health Genomics Edinburgh
    @GenomicsEdi (Twitter)

    New paper: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @RIE_Lothian https://t.co/NoWZ6wCyVh
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    June 20, 2023

  • Viral Metagenomics
    @papers_viral (Twitter)

    Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/F43MWQNVFQ https://t.co/fGAyeP1B2E
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    June 17, 2023

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  • Bloodsucking Parasites
    @bloodSparasites (Twitter)

    RT @bloodSparasites: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://…
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    June 15, 2023

    2

  • PLOS Biology
    @PLOSBiology (Twitter)

    Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhb16 https://t.co/rZ9201wXjA
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    June 15, 2023

    1

  • Kevin G. Libuit
    @kevinlibuit (Twitter)

    RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
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    June 14, 2023

    10

  • Nicole Feriancek
    @NicoleFeriancek (Twitter)

    RT @Nick_FG_Chen:
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    June 14, 2023

    4

  • russ, mph
    @aesthetics_grad (Twitter)

    RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
    view full post

    June 14, 2023

    10

  • Luc Gagne
    @lagagne01 (Twitter)

    RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
    view full post

    June 14, 2023

    1

  • aimee
    @ceniseros_a (Twitter)

    RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
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    June 14, 2023

    10

  • aimee
    @ceniseros_a (Twitter)

    RT @Nick_FG_Chen:
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    June 14, 2023

    4

  • PLOS Biology
    @PLOSBiology (Twitter)

    Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/0h4YKicqsM
    view full post

    June 14, 2023

    6

    1

  • bostonjetcharter.com
    @Gifted_udeh (Twitter)

    RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
    view full post

    June 14, 2023

    10

  • Chrispin Chaguza
    @ChrispinChaguza (Twitter)

    RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
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    June 14, 2023

    10

  • russ, mph
    @aesthetics_grad (Twitter)

    RT @Nick_FG_Chen:
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    June 14, 2023

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  • Nicholas Chen, MPH
    @Nick_FG_Chen (Twitter)

    RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
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    June 14, 2023

    10

  • PLOS Biology
    @PLOSBiology (Twitter)

    RT @Nick_FG_Chen:
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    June 14, 2023

    4

  • Nicholas Chen, MPH
    @Nick_FG_Chen (Twitter)


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    June 14, 2023

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  • PLOS Biology
    @PLOSBiology (Twitter)

    Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/ChvwaLUcxX
    view full post

    June 14, 2023

    16

    10

  • PLOS Biology
    @PLOSBiology (Twitter)

    RT @bloodSparasites: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://…
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    June 14, 2023

    2

  • Bloodsucking Parasites
    @bloodSparasites (Twitter)

    Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://t.co/k0Cc15HbAR https://t.co/EF77wpNVfW
    view full post

    June 14, 2023

    2

    2

  • micropapers
    @micro_papers (Twitter)

    Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/EK9Vk9F9RG
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    June 13, 2023

  • Phage Papers
    @Phage_Papers (Twitter)

    Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/AqYssL4aQ5
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    June 13, 2023

  • New England BioGroup
    @nebiogroup (Twitter)

    Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/Q7c5Fywfyt
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    June 13, 2023

  • Roland Baker
    @RolandBakerIII (Twitter)

    Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus (H/T @outbreakupdates) https://t.co/CyyhJ4Vh3p
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    January 18, 2023

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  • Awakened Guardian
    @Tian_A1 (Twitter)

    Exploring a novel amplicon-based mpox virus sequencing strategy [ https://t.co/tNa2RmXayP ] Chen, Chrispin Chaguza, Luc Gagne, et al. (2023 ).Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox https://t.co/qzKC8NRgJH
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    January 18, 2023

  • Outbreak Science
    @outbreaksci (Twitter)

    New preprint: Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus https://t.co/46Y6a0Bqwm #Zika #monkeypox #COVID19
    view full post

    January 16, 2023

Abstract Synopsis

  • The 2022 mpox outbreak during the COVID-19 pandemic emphasized the need for better genomic surveillance and whole-genome sequencing due to limitations in current metagenomic sequencing methods.
  • To address these limitations, researchers adapted an existing sequencing approach (PrimalSeq), which was previously used for Zika and SARS-CoV-2, to effectively sequence the human monkeypox virus.
  • Findings indicated that using amplicon-based sequencing resulted in higher genome coverage, especially for samples with lower viral DNA levels, and recommendations were made to select samples with a PCR cycle threshold below 31 for optimal results.