Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/ChvwaLUcxX
Development of an amplicon-based sequencing approach in response to the global emergence of mpox.
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Jan 2023 – Aug 2024Where it's discussed
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X / Twitter32 posts
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YouTube1 videos
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What people are saying
Several discussions underscore the importance of the publication by describing it as a 'timely' solution to mpox's rise, with words like 'response' and 'development' conveying urgency and utility. The tone varies from technical appreciation—highlighting aspects like 'primer schemes' and 'bioinformatics infrastructure'—to acknowledgment of its potential impact on public health efforts, demonstrating a mix of technical engagement and recognition of its significance.
Most discussions endorse the publication, highlighting its timely relevance and potential utility, with phrases like 'timely given' and descriptions of the method as a 'response' to mpox's emergence.
The majority of posts express strong curiosity and relevance, showing excitement about the new sequencing approach and its application for mpox detection.
Many discussions delve into methodological details, such as mentioning 'amplicon-based sequencing,' 'primer schemes,' and infrastructure compatibility, indicating active analysis and appreciation for technical aspects.
Posts suggest the publication could significantly impact future genomic surveillance and public health responses, though some are cautious and emphasize ongoing need for monitoring and validation.
Abstract in brief
- The 2022 mpox outbreak during the COVID-19 pandemic emphasized the need for better genomic surveillance and whole-genome sequencing due to limitations in current metagenomic sequencing methods.
- To address these limitations, researchers adapted an existing sequencing approach (PrimalSeq), which was previously used for Zika and SARS-CoV-2, to effectively sequence the human monkeypox virus.
- Findings indicated that using amplicon-based sequencing resulted in higher genome coverage, especially for samples with lower viral DNA levels, and recommendations were made to select samples with a PCR cycle threshold below 31 for optimal results.
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Most liked on X and BlueskySequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/0h4YKicqsM
Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development of a sequencing approach in response to the global emergence of mpox https://t.co/wlJn479wGp
Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus (H/T @outbreakupdates) https://t.co/CyyhJ4Vh3p
WHO has declared another public health emergency given mpox spread in Africa. This paper describes an affordable amplicon-based sequencing approach in response to the global emergence of #mpox developed by @VogelsChantal and a large group of coauthors https://t.co/7lWQfOJ8DC
All mentions
Enhanced Amplicon Sequencing Method for Monkeypox Virus Detection
The 2022 mpox outbreak highlighted the need for improved genomic surveillance. This video discusses how researchers adapted an existing sequencing approach to effectively analyze the human monkeypox virus, improving…
WHO has declared another public health emergency given mpox spread in Africa. This paper describes an affordable amplicon-based sequencing approach in response to the global emergence of #mpox developed by @VogelsChantal and a large group of coauthors https://t.co/7lWQfOJ8DC
RT @edin_EID:
RT @npariente: Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development o…
RT @npariente: Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development o…
Method for detection and sequencing of mpox - timely given recent increase in reporting of cases @PLOSBiology Development of a sequencing approach in response to the global emergence of mpox https://t.co/wlJn479wGp
New paper: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @RIE_Lothian https://t.co/NoWZ6wCyVh
Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/F43MWQNVFQ https://t.co/fGAyeP1B2E
RT @bloodSparasites: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://…
Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhb16 https://t.co/rZ9201wXjA
RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
RT @Nick_FG_Chen:
RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
RT @Nick_FG_Chen:
Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/0h4YKicqsM
RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
RT @Nick_FG_Chen:
RT @PLOSBiology: Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplic…
RT @Nick_FG_Chen:
Sequencing #monkeypox. A #PrimalSeq primer scheme for human mpox #virus that can be plugged into currently existing amplicon-based sequencing & bioinformatics infrastructure @Nick_FG_Chen @ChrispinChaguza @lagagne01 @GRGallagher @VogelsChantal #PLOSBiology https://t.co/i5HySjhIQE https://t.co/ChvwaLUcxX
RT @bloodSparasites: Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://…
Development of an amplicon-based sequencing approach in response to the global emergence of mpox @PLOSBiology https://t.co/k0Cc15HbAR https://t.co/EF77wpNVfW
Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/EK9Vk9F9RG
Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/AqYssL4aQ5
Development of an amplicon-based sequencing approach in response to the global emergence of mpox https://t.co/Q7c5Fywfyt
Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus (H/T @outbreakupdates) https://t.co/CyyhJ4Vh3p
Exploring a novel amplicon-based mpox virus sequencing strategy [ https://t.co/tNa2RmXayP ] Chen, Chrispin Chaguza, Luc Gagne, et al. (2023 ).Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox https://t.co/qzKC8NRgJH
New preprint: Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus https://t.co/46Y6a0Bqwm #Zika #monkeypox #COVID19