Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP.

Andrew Leduc, Luke Koury, Joshua Cantlon, Nikolai Slavov

November 2023

Synopsis of Social media discussions

The discussions collectively praise the publication for its innovative approach to single-cell proteomics, with posts emphasizing the method's scalability, speed, and use of common lab materials. For example, users refer to the study as a 'preprint of great significance' and highlight specific figures, demonstrating both enthusiasm and detailed engagement that underscore its potential to transform proteomics research.

A
Agreement
 Moderate agreement

Most discussions express strong support or positive acknowledgment of the study, highlighting its significance in the field.

I
Interest
 High level of interest

The topic clearly piques interest, with multiple posts emphasizing enthusiasm for the technical advancements.

E
Engagement
 Moderate level of engagement

Posts often reference specific figures like 'figure 5' or methodologies such as 'plexDIA,' indicating moderate but active engagement.

I
Impact
 High level of impact

Many comments describe the method as a 'game changer' or 'significant,' reflecting perceived high impact on proteomics research.

Social Mentions

YouTube

7 Videos

Bluesky

4 Posts

Twitter

8 Posts

Blogs

2 Articles

News

2 Articles

Metrics

Video Views

3,444

Total Likes

58

Extended Reach

18,651

Social Features

23

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

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Single-cell Mass Spectrometry Proteomics Data Acquisition and Analysis Techniques

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July 30, 2024

1,302 views

Advances in Single-Cell Mass Spectrometry Proteomics

Advances in Single-Cell Mass Spectrometry Proteomics

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Nikolai Slavov

July 14, 2024

1,173 views

Regulators of Protein Transport Revealed by Single-Nucleus Proteomics

Regulators of Protein Transport Revealed by Single-Nucleus Proteomics

Presentation by Dr. Jason Derks at SCP2024 discussing how singlenucleus proteomics identifies regulators of protein transport, leveraging multiplexed analysis to enhance proteome coverage and accuracy in small samples, including single human cells.

Nikolai Slavov

July 13, 2024

552 views

Parallel Sample Preparation for Single-Cell Proteomics Using nPOP

Parallel Sample Preparation for Single-Cell Proteomics Using nPOP

This workshop covers highly parallel sample preparation for multiplexed single-cell proteomics using nPOP by Dr. Josh Cantlon at SCP2024. It introduces CellenOne for isolating single-cell samples for mass spectrometry analysis.

Nikolai Slavov

July 25, 2024

267 views

Advancements in Single-Cell Proteomics with plexDIA Methodology

Advancements in Single-Cell Proteomics with plexDIA Methodology

The video discusses advancements in single-cell proteomics using mass spectrometry focusing on overcoming current limitations. plexDIA is highlighted as a framework for increasing throughput and sensitivity, significantly improving data accuracy in proteomics analysis.

Nikolai Slavov

November 22, 2025

65 views

Enhancing Single-Cell Proteomics: Techniques and Data Interpretation

Enhancing Single-Cell Proteomics: Techniques and Data Interpretation

Parallel Squared Technology Institute

April 1, 2025

48 views

Parallel Sample Preparation for Single-Cell Proteomics Using nPOP

Parallel Sample Preparation for Single-Cell Proteomics Using nPOP

This workshop covers highly parallel sample prep for multiplexed single-cell proteomics with nPOP by Dr. Josh Cantlon. It introduces CellenOne for isolating single cells for LCMS analysis, demonstrating processing over 3,000 cells daily with high accuracy.

Parallel Squared Technology Institute

April 1, 2025

38 views

  • PastelBio
    @pastelbio.bsky.social (Bluesky)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP www.nature.com/artic... --- #proteomics #prot-paper
    view full post

    August 10, 2024

  • Pastel BioScience
    @PastelBio (Twitter)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/FrVTNiCKGb --- #proteomics #prot-paper https://t.co/PXJE5eDnby
    view full post

    August 10, 2024

    6

  • Kermit Murray
    @kermitmurray (Twitter)

    (BioRxiv All) Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/hlVkSFLjKY (RSS) #BioRxiv #MassSpecRSS https://t.co/UIVbKW7Rzm
    view full post

    December 21, 2023

  • Geming (Brian) Lu
    @Brian74534732 (Twitter)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP. https://t.co/u4cMhLv8ga
    view full post

    December 16, 2023

    3

  • J of Mass Spec & Advances in the Clinical Lab
    @JMSACL (Twitter)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/hjHMUQpBQc
    view full post

    December 11, 2023

    1

  • Mass-spec@RIKEN BDR KOBE
    @bdr_massspec (Twitter)

    RT @PastelBio: Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/dRljOR5m3g --- #proteo…
    view full post

    November 29, 2023

    1

  • PastelBio
    @pastelbio.bsky.social (Bluesky)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP www.biorxiv.org/cont... --- #proteomics #prot-preprint
    view full post

    November 29, 2023

    2

    1

  • Pastel BioScience
    @PastelBio (Twitter)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/dRljOR5m3g --- #proteomics #prot-preprint https://t.co/GBhisslTel
    view full post

    November 29, 2023

    4

    1

  • MD BABU MIA, PHD
    @BabuuDx (Twitter)

    Great preprint of "Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP" from @slavovLab Look at the figure 5! https://t.co/JYGRzKDR7S
    view full post

    November 28, 2023

    3

  • bioRxiv Bioengineering
    @bioRxiv_bioE (Twitter)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://t.co/r0NF8dCQgW #biorxiv_bioE
    view full post

    November 28, 2023

  • bioRxiv Bioengineering
    @biorxiv-bioeng.bsky.social (Bluesky)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://www.biorxiv.org/content/10.1101/2023.11.27.568927v1
    view full post

    November 28, 2023

  • bioRxivpreprint
    @biorxivpreprint.bsky.social (Bluesky)

    Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP https://www.biorxiv.org/content/10.1101/2023.11.27.568927v1
    view full post

    November 28, 2023

Abstract Synopsis

  • nPOP is a high-throughput method that allows the preparation of thousands of single-cell samples in tiny droplets on glass slides, making single-cell proteomics faster and more scalable.
  • It uses mass spectrometry, specifically plexDIA, to accurately measure around 3000 to 3700 proteins per human cell, with the whole process taking only 1 or 2 days for over 3000 cells.
  • The protocol is flexible, utilizes common lab materials, and includes quality control tools, making it easier for labs to adopt and expand for large-scale single-cell protein analysis.]