Publications by authors named "Michael Themis"

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InGeTox: a human-based in vitro platform to evaluate lentivirus/host interactions that contribute to genotoxicity.
Saqlain Suleman, Sharmin Alhaque, Andrew Guo, Huairen Zhang, Annette Payne, Michael Themis

Gene Ther· December 2025

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Transcriptomic Profiling of iPS Cell-Derived Hepatocyte-like Cells Reveals Their Close Similarity to Primary Liver Hepatocytes.
Saqlain Suleman, Sharmin Alhaque, Andrew Guo, Aaron Zhang, Serena Fawaz, Michael Themis

Cells· June 2025

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Analysis of HIV-1-Based Lentiviral Vector Particle Composition by PacBio Long-Read Nucleic Acid Sequencing.
Saqlain Suleman, Mohammad S Khalifa, Serena Fawaz, Sharmin Alhaque, Yaghoub Chinea, Michael Themis

Hum Gene Ther· March 2025

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Improving the Assessment of Risk Factors Relevant to Potential Carcinogenicity of Gene Therapies: A Consensus Article.
Jan C Klapwijk, Alberto Del Rio Espinola, Silvana Libertini, Philippe Collin, Mick D Fellows, Michael Themis

Hum Gene Ther· August 2024

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Optimised protocols to generate high titre lentiviral vectors using a novel transfection agent enabling extended HEK293T culture following transient transfection and suspension culture.
Saqlain Suleman, Serena Fawaz, Terry Roberts, Stuart Ellison, Brian Bigger, Michael Themis

J Virol Methods· April 2024

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HIV- 1 lentivirus tethering to the genome is associated with transcription factor binding sites found in genes that favour virus survival.
Saqlain Suleman, Annette Payne, Johnathan Bowden, Sharmin Al Haque, Marco Zahn, Michael Themis

Gene Ther· December 2022

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Rapid and inexpensive purification of adenovirus vectors using an optimised aqueous two-phase technology.
Saqlain Suleman, Kuteiba Schrubaji, Chrysovalanto Filippou, Svetlana Ignatova, Peter Hewitson, Michael Themis

J Virol Methods· January 2022

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Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells.
Balta Lucendo-Villarin, Hassan Rashidi, Sharmin Alhaque, Lena Fischer, Jose Meseguer-Ripolles, Michael Themis

J Vis Exp· July 2019

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Modifying inter-cistronic sequence significantly enhances IRES dependent second gene expression in bicistronic vector: Construction of optimised cassette for gene therapy of familial hypercholesterolemia.
Faisal A Al-Allaf, Zainularifeen Abduljaleel, Mohammad Athar, Mohiuddin M Taher, Wajahatullah Khan, Michael Themis

Noncoding RNA Res· March 2019

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Lentivector Producer Cell Lines with Stably Expressed Vesiculovirus Envelopes.
Maha Tijani, Altar M Munis, Christopher Perry, Khaled Sanber, Marta Ferraresso, Michael Themis

Mol Ther Methods Clin Dev· September 2018

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Three-dimensional cell culture: from evolution to revolution.
Sharmin Alhaque, Michael Themis, Hassan Rashidi

Philos Trans R Soc Lond B Biol Sci· July 2018

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Genome wide classification and characterisation of CpG sites in cancer and normal cells.
Mohammadmersad Ghorbani, Michael Themis, Annette Payne

Comput Biol Med· January 2016

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Transduction of fetal mice with a feline lentiviral vector induces liver tumors which exhibit an E2F activation signature.
Reba Condiotti, Daniel Goldenberg, Hilla Giladi, Temima Schnitzer-Perlman, Simon N Waddington, Matthew Themis, Michael Themis

Mol Ther· January 2014

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Monitoring for potential adverse effects of prenatal gene therapy: mouse models for developmental aberrations and inadvertent germ line transmission.
Charles Coutelle, Simon N Waddington, Michael Themis

Methods Mol Biol· September 2012

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Monitoring for potential adverse effects of prenatal gene therapy: genotoxicity analysis in vitro and on small animal models ex vivo and in vivo.
Michael Themis

Methods Mol Biol· September 2012

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LDLR-Gene therapy for familial hypercholesterolaemia: problems, progress, and perspectives.
Faisal A Al-Allaf, Charles Coutelle, Simon N Waddington, Anna L David, Richard Harbottle, Michael Themis

Int Arch Med· December 2010

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Ultrasonographic development of the fetal sheep stomach and evaluation of early gestation ultrasound-guided in utero intragastric injection.
Anna L David, Khalil N Abi-Nader, Boaz Weisz, S W Steven Shaw, Michael Themis

Taiwan J Obstet Gynecol· March 2010

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Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype.
Nigel L Kennea, Simon N Waddington, Jerry Chan, Keelin O'Donoghue, Davy Yeung, Michael Themis

Cell Cycle· April 2009

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Widespread distribution and muscle differentiation of human fetal mesenchymal stem cells after intrauterine transplantation in dystrophic mdx mouse.
Jerry Chan, Simon N Waddington, Keelin O'Donoghue, Hitoshi Kurata, Pascale V Guillot, Michael Themis

Stem Cells· April 2007

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Overexpression of connexin 43 using a retroviral vector improves electrical coupling of skeletal myoblasts with cardiac myocytes in vitro.
Oleg Tolmachov, Yu-Ling Ma, Michael Themis, Pravina Patel, Hilmar Spohr

BMC Cardiovasc Disord· June 2006

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Coupled analysis of bacterial transformants and ligation mixture by duplex PCR enables detection of fatal instability of a nascent recombinant plasmid.
Faisal A Al-Allaf, Oleg Tolmachov, Michael Themis, Charles Coutelle

J Biochem Biophys Methods· August 2005

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In utero gene therapy: current challenges and perspectives.
Simon N Waddington, M Gabriela Kramer, Ruben Hernandez-Alcoceba, Suzanne M K Buckley, Michael Themis

Mol Ther· May 2005

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Accurate size gauging of ExoIII/S1-generated deletions by PCR analysis of ligation mixtures.
Faisal Al-Allaf, Oleg Tolmachov, Michael Themis, Charles Coutelle

Anal Biochem· April 2005

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Enhancement of adenovirus-mediated gene transfer to the airways by DEAE dextran and sodium caprate in vivo.
Lisa G Gregory, Richard P Harbottle, Lorraine Lawrence, Holly J Knapton, Michael Themis

Mol Ther· January 2003

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Restoration of LDL receptor function in cells from patients with autosomal recessive hypercholesterolemia by retroviral expression of ARH1.
Emily R Eden, Dilipkumar D Patel, Xi-Ming Sun, Jemima J Burden, Michael Themis

J Clin Invest· December 2002

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