Multiplexed single-cell proteomics using SCoPE2.

Aleksandra A Petelski, Edward Emmott, Andrew Leduc, R Gray Huffman, Harrison Specht, David H Perlman, Nikolai Slavov

December 2021 Nat Protoc

Synopsis of Social media discussions

This collection reflects enthusiasm for the SCoPE2 protocol's technical advantages, with posts highlighting its efficiency and reproducibility using phrases like 'cost-effective' and 'automation potential.' Discussions also emphasize its potential influence on biological research, using words like 'game changer' and 'advancement' to convey both excitement and recognition of its impact.

A
Agreement
 Moderate agreement

Most discussions expressed positive recognition of the publication and its protocol, indicating general agreement on its significance.

I
Interest
 High level of interest

The posts show high curiosity and enthusiasm, frequently referencing the technical details and potential of SCoPE2.

E
Engagement
 Moderate level of engagement

Participants reference specific methods, sharing resources and protocols, demonstrating moderate engagement with the study.

I
Impact
 Moderate level of impact

There is a shared perception that this protocol could significantly advance single-cell proteomics and related fields.

Social Mentions

YouTube

5 Videos

Facebook

3 Posts

Twitter

36 Posts

Blogs

3 Articles

News

4 Articles

Metrics

Video Views

10,335

Total Likes

193

Extended Reach

268,639

Social Features

51

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

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  • Prof. Nikolai Slavov
    @slavov_n (Twitter)

    The protocol at @NatureProtocols: Multiplexed single-cell proteomics using SCoPE2: https://t.co/5QyqclYVRk The protocol at @JoVEJournal: https://t.co/5dNK32ctgX Tutorials and resources: https://t.co/pafbiOZcGm https://t.co/6SY7EX9a7j
    view full post

    October 29, 2023

    3

  • Pastel BioScience
    @PastelBio (Twitter)

    RT @JamesKerwin9: Multiplexed single-cell proteomics using SCoPE2 https://t.co/Pvgu2asNCu
    view full post

    March 14, 2022

    2

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @JamesKerwin9: Multiplexed single-cell proteomics using SCoPE2 https://t.co/Pvgu2asNCu
    view full post

    March 14, 2022

    2

  • James Kerwin
    @JamesKerwin9 (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 https://t.co/Pvgu2asNCu
    view full post

    March 14, 2022

    2

  • Ed Emmott
    @edemmott (Twitter)

    RT @formulatrix: Nature Protocols: Multiplexed single-cell proteomics using SCoPE2 Single Cell Proteomics (SCoPE2) protocol was described…
    view full post

    January 25, 2022

    3

  • EKO
    @Ekoxprasetio (Twitter)

    RT @formulatrix: Nature Protocols: Multiplexed single-cell proteomics using SCoPE2 Single Cell Proteomics (SCoPE2) protocol was described…
    view full post

    January 24, 2022

    3

  • Bioinformatics Trends 
    @BinfoTrends (Twitter)

    RT @formulatrix: Nature Protocols: Multiplexed single-cell proteomics using SCoPE2 Single Cell Proteomics (SCoPE2) protocol was described…
    view full post

    January 24, 2022

    3

  • Formulatrix
    @formulatrix (Twitter)

    Nature Protocols: Multiplexed single-cell proteomics using SCoPE2 Single Cell Proteomics (SCoPE2) protocol was described which uses an isobaric carrier to enhance peptide sequence identification. Read more: https://t.co/7VYqWMl98W #NatureProtocols #SingleCell #MANTIS https://t.co/7nQS0xCDYm
    view full post

    January 24, 2022

    9

    3

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @RobotRrid: RRIDs were included in the Nature Protocols paper "Multiplexed single-cell proteomics using SCoPE2" (https://t.co/v1o18q5ySK…
    view full post

    December 12, 2021

    1

  • RRID Robot
    @RobotRrid (Twitter)

    RRIDs were included in the Nature Protocols paper "Multiplexed single-cell proteomics using SCoPE2" (https://t.co/v1o18q5ySK). Thank you for making your #methodsmatter. #ReproducibleResearch
    view full post

    December 12, 2021

    1

    1

  • Farid Babayev
    @DNARNAATP (Twitter)

    RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD
    view full post

    November 22, 2021

    5

  • Andrea Petretto
    @Angrist_Fusion (Twitter)

    RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD
    view full post

    November 22, 2021

    5

  • 川田健太郎@産総研
    @KawataKentaro (Twitter)

    RT @scell_papers: Multiplexed single-cell proteomics using SCoPE2 https://t.co/unvC3sIT4Y
    view full post

    November 4, 2021

    2

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @scell_papers: Multiplexed single-cell proteomics using SCoPE2 https://t.co/unvC3sIT4Y
    view full post

    November 4, 2021

    2

  • Single Cell papers
    @scell_papers (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 https://t.co/unvC3sIT4Y
    view full post

    November 4, 2021

    2

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @realBioMassSpec: Multiplexed single-cell proteomics using SCoPE2 #NatProtoc #MassSpec https://t.co/gKqvMe4b5U
    view full post

    October 31, 2021

    1

  • BioMassSpec
    @realBioMassSpec (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 #NatProtoc #MassSpec https://t.co/gKqvMe4b5U
    view full post

    October 31, 2021

    1

    1

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @sc_papers: Multiplexed single-cell proteomics using SCoPE2 https://t.co/lBvc2eeOzT https://t.co/I04YRcZftJ
    view full post

    October 30, 2021

    1

  • single cell papers
    @sc_papers (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 https://t.co/lBvc2eeOzT https://t.co/I04YRcZftJ
    view full post

    October 30, 2021

    1

  • PH
    @parham350 (Twitter)

    RT @nobolly: Multiplexed single-cell proteomics using SCoPE2 | Nat Protocols https://t.co/10K4Kcz3EX
    view full post

    October 29, 2021

    2

  • Mass-spec@RIKEN BDR KOBE
    @bdr_massspec (Twitter)

    RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD
    view full post

    October 29, 2021

    5

  • Stephen Holmes
    @StephenNTD (Twitter)

    RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ
    view full post

    October 29, 2021

    4

  • Giovani Veríssimo
    @Giovaniverissim (Twitter)

    RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD
    view full post

    October 29, 2021

    5

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD
    view full post

    October 29, 2021

    5

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @kermitmurray: Multiplexed single-cell proteomics using SCoPE2 https://t.co/dRnwi0jym9 #nature #MassSpecPub
    view full post

    October 29, 2021

    1

  • Pastel BioScience
    @PastelBio (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD
    view full post

    October 29, 2021

    11

    5

  • Andrew Leduc
    @_AndrewLeduc (Twitter)

    RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ
    view full post

    October 29, 2021

    4

  • Kermit Murray
    @kermitmurray (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 https://t.co/dRnwi0jym9 #nature #MassSpecPub
    view full post

    October 29, 2021

    3

    1

  • Hiro Nakagami 中神 弘史
    @Grandpa_Hiro (Twitter)

    RT @nobolly: Multiplexed single-cell proteomics using SCoPE2 | Nat Protocols https://t.co/10K4Kcz3EX
    view full post

    October 29, 2021

    2

  • Faculty of Health and Life Sciences
    @LivUniHLS (Twitter)

    RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ
    view full post

    October 29, 2021

    4

  • Tatsuya Nobori
    @nobolly (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 | Nat Protocols https://t.co/10K4Kcz3EX
    view full post

    October 29, 2021

    7

    2

  • Sonia
    @srochaliv (Twitter)

    RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ
    view full post

    October 29, 2021

    4

  • emmottlab
    @emmottlab (Twitter)

    New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ
    view full post

    October 29, 2021

    12

    4

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/mMjE3DfPWN #proteomics @emmottlab @slavovLab https://t.co/0li…
    view full post

    March 15, 2021

    1

  • Pastel BioScience
    @PastelBio (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 | https://t.co/mMjE3DfPWN #proteomics @emmottlab @slavovLab https://t.co/0li5Bm4Qay
    view full post

    March 15, 2021

    1

    1

  • bioRxiv
    @biorxivpreprint (Twitter)

    Multiplexed single-cell proteomics using SCoPE2 https://t.co/YJiNT9vBy2 #bioRxiv
    view full post

    March 15, 2021

    1

Abstract Synopsis

  • Many biological systems consist of diverse single cells, which drives the need for detailed single-cell analysis.
  • Emerging mass-spectrometry methods allow for the quantification of over 1,000 proteins per cell, enhancing specificity in protein studies.
  • The SCoPE2 protocol facilitates cost-effective, automated single-cell protein quantification using readily available equipment, allowing analysis of ~200 single cells in just 24 hours.