Nat Protoc December 2021 PMID 34716448

Multiplexed single-cell proteomics using SCoPE2.

Aleksandra A Petelski, Edward Emmott, Andrew Leduc, R Gray Huffman, Harrison Specht, David H Perlman, Nikolai Slavov

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Mentions

Peak: 18 in Oct 2021

across 5 sources since Mar 2021

Extended reach

268,639

potential audience

Total likes

193

Video views

10,335

Mentions per month

Mar 2021 – Apr 2025

Where it's discussed

X / Twitter36 posts
YouTube5 videos
News4 articles
Facebook3 posts
Blogs3 articles

AI synopsis

What people are saying

This collection reflects enthusiasm for the SCoPE2 protocol's technical advantages, with posts highlighting its efficiency and reproducibility using phrases like 'cost-effective' and 'automation potential.' Discussions also emphasize its potential influence on biological research, using words like 'game changer' and 'advancement' to convey both excitement and recognition of its impact.

AgreementModerate agreement

Most discussions expressed positive recognition of the publication and its protocol, indicating general agreement on its significance.

InterestHigh interest

The posts show high curiosity and enthusiasm, frequently referencing the technical details and potential of SCoPE2.

EngagementModerate engagement

Participants reference specific methods, sharing resources and protocols, demonstrating moderate engagement with the study.

ImpactModerate impact

There is a shared perception that this protocol could significantly advance single-cell proteomics and related fields.

Abstract in brief

Many biological systems consist of diverse single cells, which drives the need for detailed single-cell analysis.
Emerging mass-spectrometry methods allow for the quantification of over 1,000 proteins per cell, enhancing specificity in protein studies.
The SCoPE2 protocol facilitates cost-effective, automated single-cell protein quantification using readily available equipment, allowing analysis of ~200 single cells in just 24 hours.

Read the full abstract

All mentions

Advances in Single-Cell Proteomics Using Mass Spectrometry Techniques

This presentation discusses recent progress in single-cell proteomics, focusing on sample preparation and techniques like multiplexing and automation to improve sensitivity and throughput in protein analysis at the…

Nikolai Slavov · Jul 6, 2022 3,428 views

Dimensionality Reduction Techniques in Proteomics Analysis

This lecture on dimensionality reduction was presented by Prof Nikolai Slavov and is part of the course httpsslavovlabnetstats. It discusses methods for reducing data complexity, essential for analyzing high-dimensional…

Nikolai Slavov · Nov 1, 2021 3,056 views

Advances in Single-Cell Proteomics Using Mass Spectrometry Techniques

This lecture introduces single-cell proteomics by mass spectrometry, highlighting plexDIA's high-throughput multiplexing capabilities for small samples, enabling detailed protein analysis in individual human cells.

Nikolai Slavov · Nov 30, 2022 2,502 views

Single-cell Mass Spectrometry Proteomics Data Acquisition and Analysis Techniques

Workshop on single-cell mass spectrometry proteomic data acquisition using plexDIA and timsTOF, with initial interpretation via QuantQC, held at SCP2024. Focuses on improving data quality by minimizing protein leakage…

Nikolai Slavov · Jul 30, 2024 1,302 views

Enhancing Single-Cell Proteomics: Techniques and Data Interpretation

Parallel Squared Technology Institute · Apr 1, 2025 48 views

Prof. Nikolai Slavov

@slavov_n · X

Oct 29, 2023 · 3 likes · 19,979 followers View post →

Pastel BioScience

@PastelBio · X

RT @JamesKerwin9: Multiplexed single-cell proteomics using SCoPE2 https://t.co/Pvgu2asNCu

Mar 14, 2022 · 2 shares · 5,375 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @JamesKerwin9: Multiplexed single-cell proteomics using SCoPE2 https://t.co/Pvgu2asNCu

Mar 14, 2022 · 2 shares · 3,837 followers View post →

James Kerwin

@JamesKerwin9 · X

Multiplexed single-cell proteomics using SCoPE2 https://t.co/Pvgu2asNCu

Mar 14, 2022 · 2 shares · 144 followers View post →

Ed Emmott

@edemmott · X

RT @formulatrix: Nature Protocols: Multiplexed single-cell proteomics using SCoPE2 Single Cell Proteomics (SCoPE2) protocol was described…

Jan 25, 2022 · 3 shares · 3,728 followers View post →

EKO

@Ekoxprasetio · X

RT @formulatrix: Nature Protocols: Multiplexed single-cell proteomics using SCoPE2 Single Cell Proteomics (SCoPE2) protocol was described…

Jan 24, 2022 · 3 shares · 443 followers View post →

Bioinformatics Trends 

@BinfoTrends · X

RT @formulatrix: Nature Protocols: Multiplexed single-cell proteomics using SCoPE2 Single Cell Proteomics (SCoPE2) protocol was described…

Jan 24, 2022 · 3 shares · 8,158 followers View post →

Formulatrix

@formulatrix · X

Jan 24, 2022 · 9 likes · 3 shares · 661 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @RobotRrid: RRIDs were included in the Nature Protocols paper "Multiplexed single-cell proteomics using SCoPE2" (https://t.co/v1o18q5ySK…

Dec 12, 2021 · 1 share · 3,837 followers View post →

RRID Robot

@RobotRrid · X

RRIDs were included in the Nature Protocols paper "Multiplexed single-cell proteomics using SCoPE2" (https://t.co/v1o18q5ySK). Thank you for making your #methodsmatter. #ReproducibleResearch

Dec 12, 2021 · 1 like · 1 share · 161 followers View post →

Farid Babayev

@DNARNAATP · X

RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD

Nov 22, 2021 · 5 shares · 3,551 followers View post →

Andrea Petretto

@Angrist_Fusion · X

RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD

Nov 22, 2021 · 5 shares · 580 followers View post →

川田健太郎@産総研

@KawataKentaro · X

RT @scell_papers: Multiplexed single-cell proteomics using SCoPE2 https://t.co/unvC3sIT4Y

Nov 4, 2021 · 2 shares · 141 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @scell_papers: Multiplexed single-cell proteomics using SCoPE2 https://t.co/unvC3sIT4Y

Nov 4, 2021 · 2 shares · 3,837 followers View post →

Single Cell papers

@scell_papers · X

Multiplexed single-cell proteomics using SCoPE2 https://t.co/unvC3sIT4Y

Nov 4, 2021 · 2 shares · 11,517 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @realBioMassSpec: Multiplexed single-cell proteomics using SCoPE2 #NatProtoc #MassSpec https://t.co/gKqvMe4b5U

Oct 31, 2021 · 1 share · 3,837 followers View post →

BioMassSpec

@realBioMassSpec · X

Multiplexed single-cell proteomics using SCoPE2 #NatProtoc #MassSpec https://t.co/gKqvMe4b5U

Oct 31, 2021 · 1 like · 1 share · 5,995 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @sc_papers: Multiplexed single-cell proteomics using SCoPE2 https://t.co/lBvc2eeOzT https://t.co/I04YRcZftJ

Oct 30, 2021 · 1 share · 3,837 followers View post →

single cell papers

@sc_papers · X

Multiplexed single-cell proteomics using SCoPE2 https://t.co/lBvc2eeOzT https://t.co/I04YRcZftJ

Oct 30, 2021 · 1 share · 305 followers View post →

@parham350 · X

RT @nobolly: Multiplexed single-cell proteomics using SCoPE2 | Nat Protocols https://t.co/10K4Kcz3EX

Oct 29, 2021 · 2 shares · 174 followers View post →

Mass-spec@RIKEN BDR KOBE

@bdr_massspec · X

RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD

Oct 29, 2021 · 5 shares · 68 followers View post →

Stephen Holmes

@StephenNTD · X

RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ

Oct 29, 2021 · 4 shares · 115 followers View post →

Giovani Veríssimo

@Giovaniverissim · X

RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD

Oct 29, 2021 · 5 shares · 65 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD

Oct 29, 2021 · 5 shares · 3,837 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @kermitmurray: Multiplexed single-cell proteomics using SCoPE2 https://t.co/dRnwi0jym9 #nature #MassSpecPub

Oct 29, 2021 · 1 share · 3,837 followers View post →

Pastel BioScience

@PastelBio · X

Multiplexed single-cell proteomics using SCoPE2 | https://t.co/GZYgQnN7dZ #proteomics https://t.co/y6sUwde0XD

Oct 29, 2021 · 11 likes · 5 shares · 5,375 followers View post →

Andrew Leduc

@_AndrewLeduc · X

RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ

Oct 29, 2021 · 4 shares · 616 followers View post →

Kermit Murray

@kermitmurray · X

Multiplexed single-cell proteomics using SCoPE2 https://t.co/dRnwi0jym9 #nature #MassSpecPub

Oct 29, 2021 · 3 likes · 1 share · 1,969 followers View post →

Hiro Nakagami 中神弘史

@Grandpa_Hiro · X

RT @nobolly: Multiplexed single-cell proteomics using SCoPE2 | Nat Protocols https://t.co/10K4Kcz3EX

Oct 29, 2021 · 2 shares · 2,343 followers View post →

Faculty of Health and Life Sciences

@LivUniHLS · X

RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ

Oct 29, 2021 · 4 shares · 3,259 followers View post →

Tatsuya Nobori

@nobolly · X

Multiplexed single-cell proteomics using SCoPE2 | Nat Protocols https://t.co/10K4Kcz3EX

Oct 29, 2021 · 7 likes · 2 shares · 8,193 followers View post →

Sonia

@srochaliv · X

RT @emmottlab: New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ

Oct 29, 2021 · 4 shares · 1,221 followers View post →

emmottlab

@emmottlab · X

New paper: Multiplexed single-cell proteomics using SCoPE2. https://t.co/SOiZ9hSjvJ

Oct 29, 2021 · 12 likes · 4 shares · 1,060 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @PastelBio: Multiplexed single-cell proteomics using SCoPE2 | https://t.co/mMjE3DfPWN #proteomics @emmottlab @slavovLab https://t.co/0li…

Mar 15, 2021 · 1 share · 3,837 followers View post →

Pastel BioScience

@PastelBio · X

Multiplexed single-cell proteomics using SCoPE2 | https://t.co/mMjE3DfPWN #proteomics @emmottlab @slavovLab https://t.co/0li5Bm4Qay

Mar 15, 2021 · 1 like · 1 share · 5,375 followers View post →

bioRxiv

@biorxivpreprint · X

Multiplexed single-cell proteomics using SCoPE2 https://t.co/YJiNT9vBy2 #bioRxiv

Mar 15, 2021 · 1 like · 136,858 followers View post →

Multiplexed single-cell proteomics using SCoPE2.

Mentions per month

Where it's discussed

What people are saying

Abstract in brief

Top posts

All mentions

Advances in Single-Cell Proteomics Using Mass Spectrometry Techniques

Dimensionality Reduction Techniques in Proteomics Analysis

Advances in Single-Cell Proteomics Using Mass Spectrometry Techniques

Single-cell Mass Spectrometry Proteomics Data Acquisition and Analysis Techniques

Enhancing Single-Cell Proteomics: Techniques and Data Interpretation