Prioritized mass spectrometry increases the depth, sensitivity and data completeness of single-cell proteomics.

R Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia, Francesco Borriello, Harrison Specht, Jason Derks, Saad Khan, Luke Khoury, Edward Emmott, Aleksandra A Petelski, David H Perlman, Jürgen Cox, Ivan Zanoni, Nikolai Slavov

May 2023 Nat Methods

Synopsis of Social media discussions

Several discussions celebrate the article's breakthrough in increasing data depth, with phrases like 'excited for collaboration' and 'revolutionizes proteomics,' demonstrating high interest and engagement. The tone reflects excitement about the method's potential to significantly advance the field, suggesting a consensus on its high impact and importance.

A
Agreement
 Strong agreement

Most discussions strongly endorse the significance of the research, emphasizing its potential to advance proteomics capabilities.

I
Interest
 High level of interest

The posts express high interest by highlighting collaborations, new methodologies, and excitement about the technological advances in single-cell proteomics.

E
Engagement
 High engagement

Participants actively discuss specifics like increased proteome depth and reduced analysis time, indicating deep engagement with the topic.

I
Impact
 High level of impact

The majority of posts suggest that this method could be transformative for biological research, emphasizing its broad implications and practical applications.

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37 Posts

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2 Articles

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  • Dr. Gennadi Glinsky, MD, Ph.D.
    @gglinskii (Twitter)

    Prioritized mass spectrometry increases the depth, sensitivity and data completeness of single-cell proteomics https://t.co/Iv0aK4P0M0
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    October 16, 2024

  • Chenzhen Zhang
    @ChenzhenZhang (Twitter)

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    April 26, 2023

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  • Northeastern University Bioengineering
    @NUBioE1 (Twitter)

    Our BioE Professors are always keeping busy! Here are some recent articles: @BajpayeeLab @ChenzhenZhang - https://t.co/KKyWzXaAIJ @slavovLab - https://t.co/JLqnKLZA96 Kiran Vanaja - https://t.co/1eNv4z7f8d & https://t.co/49PaUHfHOv Rai Winslow - https://t.co/P867bMfct6 https://t.co/4nPWjWOlmg
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    @FbioFon84085623 (Twitter)

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  • Alice Bertocchi
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    @lum_lab (Twitter)

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    April 3, 2023

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    @kermitmurray (Twitter)

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    @Marco_Di_Gioia (Twitter)

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    @TTCooper_PhD (Twitter)

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    @_JasonDerks (Twitter)

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    @nobolly (Twitter)

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Abstract Synopsis

  • Prioritized mass spectrometry (pSCoPE) enhances single-cell proteomics by increasing data sensitivity, completeness, and depth, allowing for more precise analysis of proteins and their modifications.
  • This method analyzes thousands of selected peptides across individual cells, doubling the amount of measurable proteome data and enabling detailed insights into protein variation, functional relationships, and enzymatic activities in different treatment conditions.
  • pSCoPE is freely accessible, applicable to various biological studies, and helps researchers focus on proteins of interest without compromising proteome coverage.]